A method of controlling corn armyworm by using nano silicon quantum dots
A technology of nano-silicon quantum dots and corn armyworm, applied in pest control, nanotechnology, chemical instruments and methods, etc., can solve the problems of heavy metal toxicity, environmental pollution, etc., increase biomass, improve total phenols and chlorogenic The effect of increasing the content of chlorogenic acid and increasing the content of chlorogenic acid
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Embodiment 1
[0035] A preparation method of nano-silicon quantum dots, comprising the steps of:
[0036] Weigh 2.3g of ascorbic acid and dissolve it in 8.0mL of water, then add 2.0mL of N-aminoethyl-3-aminopropylmethyldimethoxysilane, mix well at 80°C, and then stir in a water bath at 80°C for 8h to react; After the reaction, use a dialysis bag (1kDa, molecular weight cut-off) to remove excess reactants; then the solution after dialysis was centrifuged at 4°C, 10000r / min for 20min; the precipitate was dried to obtain nano-silicon quantum dots.
[0037] The size of the nano-silicon quantum dots obtained is 5nm, and the shape is spherical (such as figure 1 ), the surface has functional groups such as -OH and -NH ( figure 2 ), the hydrated diameter is about 42.9nm, and the surface charge is 10.6eV.
Embodiment 2
[0039] A method for controlling corn armyworm using nanometer silicon quantum dots, comprising the steps of:
[0040] (1) Disinfect corn seeds (Suyu 29) from Jiangsu Academy of Agricultural Sciences in 5% sodium hypochlorite solution for 10 minutes, then rinse with deionized water 3 times for disinfection;
[0041] (2) After the disinfection is completed, soak the corn seeds in deionized water for 4 hours, then put the corn seeds into a petri dish with damp filter paper, cultivate them under dark conditions in the greenhouse, and spray water regularly every day;
[0042] (3) After cultivating for 5 days, select the corn seeds that germinate evenly, and transfer to the pots and pots that 1.0Kg of soil is housed to grow;
[0043](4) When the corn seedling grows to three leaves and one heart, the aqueous solution 20mL of the nano-silicon quantum dot (embodiment 1) of 50mg / L is sprayed on the leaf surface; Worm (Keyun Biology), the growth and development of armyworm were evaluate...
Embodiment 3
[0046] Adjust the concentration of the aqueous solution of nano-silicon quantum dots (embodiment 1) in embodiment 2 to be 10, 150mg / L, and others are consistent with embodiment 2.
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