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Ainsliaea fragrans germplasm improvement method

A technology for apricot fragrant rabbit ear wind and germplasm, which is applied in the directions of planting substrates, horticultural methods, botanical equipment and methods, etc., and can solve the problems that the content of active ingredients is not particularly prominent, and the yield of apricot fragrant rabbit ear wind is low.

Active Publication Date: 2021-11-05
SOUTHWEST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the disadvantages of low yield and low content of active ingredients in the existing varieties of Xingxiang Rabbit’s Ear, the present invention uses modern biological breeding technology to double the chromosomes of Xingxiang’s Rabbit’s Ear, improving the yield per plant of Xingxiang Rabbit’s Ear and the green Content of active ingredients such as ortho acid and protocatechuic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for germplasm improvement of apricot-flavored rabbit ears, comprising the following steps,

[0033] A. Germplasm screening: select high-quality germplasm with high content of active ingredients and large biomass per plant as female parent;

[0034] B. Germplasm improvement;

[0035] B1 material cleaning: select healthy young stems free from diseases and insect pests, rinse with soapy water for 20 minutes, rinse with water 4 times, drop 2 drops of Tween-80, shake for 10 minutes, rinse with tap water for 60 minutes;

[0036] B2 Aseptic system establishment: Transfer the cleaned materials to the ultra-clean workbench, first disinfect with 75% alcohol for 10 seconds, rinse with sterile water twice, and disinfect with 2 drops of 0.1% mercury solution of Tween-80 for 6 minutes , rinsed with sterile water 6 times, absorbed the surface moisture on the sterile filter paper, removed the wounds at both ends after disinfection, cut it into 1.0cm-sized small sections with ...

Embodiment 2

[0044] A method for germplasm improvement of apricot-flavored rabbit ears, comprising the following steps,

[0045] A. Germplasm screening: select high-quality germplasm with high content of active ingredients and large biomass per plant as female parent;

[0046] B. Germplasm improvement;

[0047] B1 material cleaning: select healthy young stems free of diseases and insect pests, rinse with soapy water for 20 minutes, rinse with water for 5 times, drop 3 drops of Tween-80, shake for 15 minutes, and rinse with tap water for 80 minutes;

[0048] B2 Aseptic system establishment: Transfer the cleaned materials to the ultra-clean workbench, first disinfect with 75% alcohol for 15 seconds, rinse with sterile water for 3 times, and disinfect with 2 drops of 0.1% mercury solution of Tween-80 for 7 minutes , rinsed with sterile water 7 times, absorb the surface moisture on the sterile filter paper, remove the wounds at both ends after disinfection, cut it into 2.0cm-sized bud segment...

Embodiment 3

[0056] A method for germplasm improvement of apricot-flavored rabbit ears, comprising the following steps,

[0057] A. Germplasm screening: select high-quality germplasm with high content of active ingredients and large biomass per plant as female parent;

[0058] B. Germplasm improvement;

[0059] B1 material cleaning: select healthy young stems free from diseases and insect pests, rinse with soapy water for 20 minutes, rinse with water 4 times, drop 2 drops of Tween-80, shake for 13 minutes, rinse with tap water for 70 minutes;

[0060] B2 Aseptic system establishment: Transfer the cleaned materials to the ultra-clean workbench, first disinfect with 75% alcohol for 13 seconds, rinse with sterile water for 3 times, and disinfect with 2 drops of 0.1% mercury solution of Tween-80 for 6.5 min, rinse with sterile water 7 times, absorb the surface moisture on the sterile filter paper, remove the wounds at both ends after disinfection, cut them into 1.5cm-sized small sections with...

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Abstract

The invention relates to a germplasm improvement method for traditional Chinese medicinal materials. The germplasm improvement method comprises the steps of A germplasm selection, B germplasm improvement, C improved germplasm identification and screening, D improved germplasm rooting, E rooted seedling domestication and F improved germplasm seed obtaining. According to the method, colchicine is used for conducting polyploid induction on ainsliaea fragrans sterile adventitious buds, then screening and purification culture are conducted through a morphological identification method and a chromosome tabletting method, the improved ainsliaea fragrans germplasm is obtained, and the obtaining rate is 19%; according to the obtained improved ainsliaea fragrans germplasm, stems are obviously thickened, leaves are obviously thickened, the single plant biomass is 1.9 times higher than that of an original female parent, the yield per mu is 1.6 times higher than that of the original female parent, meanwhile, the chlorogenic acid content is 1.3 times higher than that of the female parent, and the protocatechuic acid content is 2.2 times higher than that of the original female parent; and the obtained high-quality germplasm has genetic stability, and plants planted through the seeds still have fertility.

Description

technical field [0001] The invention relates to a method for improving germplasm of Chinese medicinal materials, which belongs to the field of plant crop breeding and planting of Chinese medicinal materials, in particular to a method for improving germplasm of apricot-scented rabbit ears. Background technique [0002] Ainsliaea fragrans Champ is a plant of the genus Ainsliaea fragrans Champ in the family Asteraceae. The whole herb is used as medicine. It has the effects of clearing away heat and detoxification, promoting dampness, and stopping bleeding. It is mainly used to treat lung abscess, mastitis, malnutrition, osteomyelitis, and snake bite hurt. Apricot rabbit ear wind is mainly distributed in Taiwan, Fujian, Zhejiang, Anhui, Jiangsu, Jiangxi, Hubei, Sichuan, Hunan, Guangdong, Guangxi, Yunnan and other provinces. In the border grass, Yunnan is also distributed near the altitude of 2000 meters. Apricot Rabbit Ear Wind contains chlorogenic acid, apigenin, quercetin, l...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H6/14A01G24/28A01G24/25A01G24/10A01G22/00
CPCA01H4/002A01H4/008A01H6/14A01G24/28A01G24/25A01G24/10A01G22/00
Inventor 邓国宾曾祥飞向建英张媛张文娟
Owner SOUTHWEST FORESTRY UNIVERSITY
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