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Auxiliary selection markers for fat weight and carcass weight of goose liver for liver and a method for selection using molecular markers

A technology for assisted selection and goose belly fat, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., to reduce workload, reduce measurement costs, and improve breeding efficiency.

Active Publication Date: 2021-07-16
YANGZHOU UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the problem of marker-assisted selection of goose belly fat weight and carcass weight for liver, and provides an auxiliary selection marker for goose belly fat weight and carcass weight for liver and a method for using molecular marker-assisted selection

Method used

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  • Auxiliary selection markers for fat weight and carcass weight of goose liver for liver and a method for selection using molecular markers
  • Auxiliary selection markers for fat weight and carcass weight of goose liver for liver and a method for selection using molecular markers
  • Auxiliary selection markers for fat weight and carcass weight of goose liver for liver and a method for selection using molecular markers

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Embodiment Construction

[0023] Take a batch of 66 Langde geese as example to set forth the present invention below:

[0024] 1) Select the individuals of Lande geese to be tested: 66 healthy individuals with uniform body weight were selected from a group of Lande geese.

[0025] 2) Blood extraction and DNA extraction: 0.1 mL of blood was extracted from the wing vein of the individual to be tested. Using a DNA extraction kit, extract the DNA template.

[0026] 3) Polymerase chain reaction and product detection: Use the extracted DNA as the template in PCR and the provided PCR kit (see attached table 1 for the list of reagents included) to carry out PCR (see attached table 2 for the reaction system, reaction See attached table 3 for details). Take 5 μL of the product and carry out 1.5% agarose gel electrophoresis, if there is a single band with a size of 912bp (see attached figure 1 ), send the PCR product to the sequencing company for testing.

[0027] 4) Sequencing and determining the genotype: s...

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Abstract

The invention belongs to the technical field of poultry breeding, and specifically relates to an auxiliary selection marker for goose belly fat weight and carcass weight for liver, which has a SNP site, and the SNP site is located at the 807th bp downstream of the start codon of the MC5R gene. The invention also discloses a method for using molecular markers to assist the selection of goose belly fat weight and carcass weight traits for liver use: select the Lande goose individual to be tested, draw blood and extract DNA, and use the provided kit to perform polymerase chain reaction (PCR) and detect the product, send the PCR product to the sequencing company for sequencing and determine the genotype, and select qualified individuals according to the genotype for breeding. The invention can not only avoid the high cost and heavy workload caused by the slaughter measurement method, and the disadvantages that excellent individuals cannot be reserved for breeding, but also can be used for large-scale selection and speed up the breeding process.

Description

technical field [0001] The invention belongs to the technical field of poultry breeding, and in particular relates to a method of using candidate gene nucleotide polymorphisms to assist in the selection of abdominal fat weight and carcass weight of fat geese to breed high-quality fat geese. It can be used by fat gras goose breeding enterprises and research units. Background technique [0002] The liver production performance of geese varies greatly among different breeds and within the same breed, indicating that genetic factors are important influencing factors. Therefore, the production performance of fat gras goose can be improved by means of genetic breeding, but the effect of phenotypic selection and slaughter test on the improvement of liver performance is not good or the cost is high. In contrast, marker-assisted selection can not only avoid the high cost and indirect selection problems caused by slaughtering live poultry, but also significantly improve the accuracy ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘龙耿拓宇刘同君赵盼龚道清
Owner YANGZHOU UNIV
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