The invention relates to the technical field of vaccine production, in particular to an automatic inactivation system capable of guaranteeing sterility of a concentrated solution and an inactivation effect in vaccine production. The system comprises a kettle body and a concentrated solution tank, a motor is arranged below the kettle body, an output shaft of the motor is connected with a stirring rod extending into the kettle body, a controller is mounted on one side of the kettle body, the upper part of the kettle body communicates with a solution pipe, the other end of the solution pipe extends into the concentrated solution tank, the inside of the concentrated solution tank communicates with an air pipe, a charging machine is fixed and mounted at the top end of the kettle body and comprises a fixed cover and an electric push rod, a push rod end of the electric push rod is fixedly connected with a solution storage box, a solution inlet groove is formed in one side of the upper end ofthe solution storage box, a solution addition tank is fixed and mounted in the fixed cover through a support, the bottom end of the solution addition tank communicates with a solution discharge pipe extending into the solution inlet groove, the top end of the solution addition tank is connected with a solution inlet pipe and an automatic solution addition pipe, so that automatic and efficient inactivation can be realized in the whole inactivation process, the inactivation efficiency is improved, and the inactivation effect is improved.