The invention discloses a single-
sprayer multi-source biological 3D (three-dimension) printing device and a method thereof, and belongs to the field of
tissue engineering and biological 3D printing. The single-
sprayer multi-source biological 3D printing device comprises a motor fixing base, an
electric motor, an active gear, a fixing rotary shaft, a turntable joining piece, a
sprayer body, a unidirectional connecting valve, a spring sheet, a feeding
pipe joint and other structures; the fixing rotary shaft is installed and fixed, the
electric motor drives a turntable to rotate through a
gear transmission belt; the unidirectional connecting valve on the turntable is clamped and pressed through two spring sheets, a
height difference delta h forms between the unidirectional connecting valve and the sprayer body; when the unidirectional connecting valve is rotated to the sprayer body, the sprayer body props up the unidirectional connecting valve, and a
steel ball is propped up to connect the sprayer body with the unidirectional connecting valve; the spring sheet is popped up to press the unidirectional connecting valve; meanwhile,
biological materials enter through the feeding
pipe joint so as to realize the printing process. Through rotating the turntable, the printing material can be switched to print multiple
biological materials with a single sprayer. The single-sprayer multi-source biological 3D (three-dimension) printing device is simple and ingenious in structure, reduces cost, and is easy to realize the flexible control; besides, the printing efficiency is greatly improved.