The invention discloses a florfenicol suspension injection. The florfenicol suspension injection is prepared from the following components in parts by weight of 10 to 30 parts of florfenicol, 60 to 80 parts of ethyl oleate, 1 to 5 parts of a surfactant, 0.1 to 0.9 part of polyoxyethylene ether (40) hydrogenated castor oil, 0.5 to 1.2 parts of span 80, 0.2 to 0.9 part of Tween 80, 0.1 to 0.3 part of poloxamer and 0.1 to 0.9 part of an antioxidant. The preparation process comprises the following steps of firstly, putting ethyl oleate into a round-bottom flask, and heating at 150 DEG C for 2 hours; cooling to 70 DEG C, and separately adding the antioxidant, the surfactant and a wetting agent while stirring; after uniformly stirring, adding florfenicol; and finally, stirring at a high speed for 30 minutes, adding all the dissolved materials into a colloid mill, carrying out colloid milling, sub-packaging the solution, and sealing to obtain a product which is wide in antibacterial range, high in antibacterial activity, small in administration frequency, small in stress, long in slow release time and low in price.