The invention provides a method for preparing a conductive composite
nanofiber nervous tissue engineering scaffold based on
graphene. The method includes the following steps: a step 1, dissolving tussur silk
fibroin and poly (
lactic acid)-poly
caprolactone in a
solvent with stirring till complete
dissolution of the tussur silk
fibroin and the poly (
lactic acid)-poly
caprolactone so as to acquiring a
spinning solution; a step 2, performing electrostatic
spinning on the
spinning solution obtained from the step 1 so as to acquire a
nanofiber membrane, performing steam
fumigation treatment by using ethyl
alcohol, and performing
drying so as to acquire tussur silk
fibroin / poly (
lactic acid)-poly
caprolactone composite nanofibers; and a step 3, dipping the tussur silk fibroin / poly (lactic acid)-poly caprolactone composite
nanofiber scaffold material obtained from the step 2 in a
graphene oxide dispersion liquid, taking out the tussur silk fibroin / poly (lactic acid)-poly caprolactone composite
nanofiber scaffold material, cleaning the tussur silk fibroin / poly (lactic acid)-poly caprolactone composite
nanofiber scaffold material, soaking the tussur silk fibroin / poly (lactic acid)-poly caprolactone composite
nanofiber scaffold material in an
ascorbic acid solution, taking out the tussur silk fibroin / poly (lactic acid)-poly caprolactone composite nanofiber
scaffold material, and cleaning the tussur silk fibroin / poly (lactic acid)-poly caprolactone composite nanofiber
scaffold material so as to acquiring the conductive composite nanofiber
nervous tissue engineering scaffold based on the
graphene. The method is simple to operate, is excellent in
repeatability, and can provide a new thought for nerve defect repairing.