50results about How to "Enables fluorescence detection" patented technology

The invention provides a capillary array electrophoresis detection method based on spatial correction and spectral correction, and the method comprises the following steps: (1) utilizing a Raman spectrum to position imaging positions of all capillaries in a capillary array on a charge coupled element; (2) performing spectrum unscrambling on multi-color fluorescent dyes in each capillary through the spatial correction; and (3) performing analysis on DNA (deoxyribonucleic acid) fragments of electrophoresis of the capillary array. In the method, the Raman spectrum of laser excitation capillary quartz or solvent water in the capillaries is adopted for realizing spectral imaging, thereby realizing the position positioning and parallelism adjustment of the capillaries on a CCD (charge coupled device); a fluorescent dye combined matrix is adopted for realizing simultaneous detection of the multi-color fluorescent dyes in the single capillary and the pixel combination way is adopted for improving the stability of a distribution matrix, reducing the amount of calculation and improving the detection stability; and a device for implementing the method is simple in structure and easy to operate.

The invention discloses a fluorescent aptamer probe based on Prussian blue nanoparticles as well as a preparation method and application of the fluorescent aptamer probe. The fluorescent aptamer probeis composed of Prussian blue nanoparticle complex modified FAM fluorophore aptamers. The preparation method comprises the following steps: incubating the Prussian blue nanoparticles and the aptamersmodified with the FAM fluorophore in an HEPEs buffer solution system in a dark place, and closing by adopting BSA, thereby obtaining the fluorescent aptamer probe. The fluorescent aptamer probe can beused for detecting various markers such as markers of tumor, breast cancer, blood glucose and Alzheimer's disease, has the advantages of strong signal, high specificity, high sensitivity, wide detection concentration range, good biological safety and the like, and is beneficial to popularization and application.

The invention belongs to the technical field of biology, and particularly relates to a molecular marker detection method, and particularly relates to a method for detecting molecular markers based ona KASP technology. The molecular markers are non-SNP and InDels. The method comprises the steps that KASP primers are designed in marker amplification target DNA sequences; for a co-dominant marker, three primers need to be designed for two target DNA sequences, and the three primers comprise two site specific primers and one common primer; for a dominant marker, two primers need to be designed for only one target DNA sequence, and the two primers comprise one site specific primer and one common primer; and the 3' end of each site specific primer is an SNP site, the Tm value of the primers is1 degree or above higher than that of a KASP joint sequence, and the size of an amplification fragment is 170 +/-130bp. According to the method, the detection range of the KASP technology is widened,and STS or SCAR is quickly detected.

The invention discloses a ratiometric fluorescent probe compound and a method used for detecting zinc ion ratio in aqueous solution, and belongs to the field of fluorescent probe. The chemical molecular formula of the ratiometric fluorescent probe compound used for detection zinc ions is C20H16N3O3Cl, and is prepared through derivatization of 2-(2'-hydroxyphenyl) benzoxazole compound which is taken as a matrix structure. The ratiometric fluorescent probe compound is high in selectivity on zinc ions, wide in detection range, high in sensitivity, excellent in anti-influence capacity on other metal ions, and is suitable for detection of Zn<2+> in aqueous solutions in the fields of biochemistry and environment chemistry.

The invention discloses a detection device adopting lased-induced liquid fluorescence and relates to the technical field of fluorescence spectra. The detection device comprises an automatic injected sample cleaning system, a fluorescence excitation system and a beam splitting system, wherein the automatic injected sample cleaning system is located in the middle of the whole device, and the fluorescence excitation system and the beam splitting system are located on two sides of the automatic injected sample cleaning system respectively. A liquid-core optical fiber is filled with a to-be-measured liquid, fluorescence produced by excitation is transmitted in the liquid-core optical fiber along with incident light and accumulated and intensified continuously, a laser is all limited in the whole liquid-core optical fiber to be transmitted after coupled to enter the liquid-core optical fiber, exciting light interacts with the to-be-detected liquid continuously, the total effective exciting light path is extended, and the intensity of the fluorescence produced by excitation is accumulated continuously. The liquid-core optical fiber can be very long, so that the intensity of the fluorescence can be increased substantially in the optical fiber, and fluorescence detection of low-concentration substances can be realized.

The invention relates to an active targeting type ultrasonic/fluorescence bimodal contrast medium as well as a preparation method and application thereof. Specifically, the contrast medium comprises nano microbubbles and targeting molecules, wherein the nano microbubbles are filled with an inner water phase; and the targeting molecules are coupled with the surfaces of the nano microbubbles. The invention further discloses a preparation method and application of the contrast medium. The contrast medium is excellent in biocompatibility and relatively small in particle size, and can not only exist and be used stably, but also effectively permeate into tumor cells, and thus targeting radiography upon tumor cells is achieved. The preparation method is simple in process and economic and safe.

The invention discloses a triarylboron conjugated polymer porous material as well as a preparation method and application thereof. The invention provides a novel preparation method of a triaryl conjugated polymer porous material, i.e., the novel triaryl conjugated polymer porous material is prepared by using a boron-tin exchange reaction as a polymerization means. The defects of high cost, difficulty in purification and the like in a preparation method of carbon-carbon coupling reaction catalyzed by noble metal are overcome, and the method is more economical and efficient. The triaryl conjugated polymer porous material disclosed by the invention can realize fluorescence detection on organic ammonia, the fluorescence quenching ratios of pyridine to P2-Th, P2-Th2 and P2-Th3 of the triaryl conjugated polymer porous material disclosed by the invention are 83.8%, 73.5% and 41.3% respectively, and the triaryl conjugated polymer porous material disclosed by the invention has a good application prospect in the field of fluorescence detection.

The invention discloses a cysteine detection reagent and a preparation method thereof. The cysteine detection reagent has the general structure as shown in the formula (please see the formula in the description), wherein R1 is H, Cl or OCH3, R2 is H, Cl or OCH3. The detection reagent does not have biological toxicity and is high in sensitivity to cysteine, cysteine can be selectively detected, fluorescent detection can be performed on cysteine in cells, and the cysteine detection reagent meets the commercialized application requirement.

The invention provides a multichannel microfluidic optical detection system in the technical field of fluorescence detection. The multichannel microfluidic optical detection system comprises a light source, a multichannel microfluidic module, a light source coupling control module, a liquid path control module, a light-electricity conversion module and a detection analysis module, wherein the light source is used for outputting optical signals, and one light source is available; the multichannel microfluidic module is provided with at least two microchannels; the light source coupling controlmodule is used for inputting the optical signals to each microchannel in a distribution manner; the liquid path control module is used for controlling the generation frequency and flow velocity of microdrops in each microchannel; the light-electricity conversion module is used for receiving the optical signals passing through the microchannels, generating electric signals, and amplifying the electric signals; and the detection analysis module is electrically connected with the light-electricity conversion module for analyzing the amplified electric signals, and thus a detection result is obtained. For the multichannel microfluidic optical detection system provided by the invention, the single light source is adopted for realizing the detection for multiple channels, and meanwhile, the increase of power of the light source due to the increase of the number of the channels is also avoided.

The embodiment of the invention discloses a fluorescence detection device, which comprises a light source module, a reaction module and a detection module, wherein the light source module comprises a plurality of monochromatic source components, a plurality of monochromatic source moving tracks, a first driving component and a first control component; the first control component is used for determining a target monochromatic source component from the monochromatic source components based on a selection instruction so as to output a moving instruction to the first driving component, and the first driving component responds to the moving instruction to drive the target monochromatic source component to move to the final position in the corresponding monochromatic source moving track; and the first control component controls the target monochromatic source component to start after the target monochromatic source component moves to the final position so as to output the target exciting light. According to the embodiment of the invention, the plurality of monochromatic light source components are connected with the plurality of sliding rails, the first driving component and the first control component, so that different monochromatic light sources can be selected to provide exciting light with different wavelengths, and fluorescence detection is carried out.

The invention discloses a method for the fluorescence detection of Alzheimer's disease markers based on a silver nano-cluster probe containing repeated AGGGTT sequences. The method comprises the following steps: synthesizing a silver nanocluster by using DNA1 as a template, specifically binding the DNA1 with DNA2, binding the bound DNA1 and DNA2 with copper ions to obtain a DNA1/DNA2-AgNCs-Cu<2+> probe, and using the DNA1/DNA2-AgNCs-<2+> fluorescence probe as a probe for detecting the Alzheimer's disease markers to detect the content of the Alzheimer's disease markers Abeta 1-40 monomers and Abeta1-40 oligomers through the change of fluorescence signals. The method realizes the fluorescence detection of the Alzheimer's disease markers, and has the advantages of stable signal, strong resistance to environmental impact, high sensitivity, low detection limit, good linear relationship, and easiness in operation.

The invention discloses a pollution source analysis method and device and a storage medium, and belongs to the technical field of water environment protection and monitoring. The method comprises the following steps: detecting a water sample by adopting a three-dimensional fluorescence spectrum detection method and an electrochemical spectrum detection method to obtain multi-dimensional spectrum data; carrying out dimension reduction processing on the multidimensional spectral data by utilizing an entropy weight method, and respectively calculating distribution weights of atlas matrixes; distributing weights based on the atlas matrix, and constructing a two-dimensional weight matrix; and calculating the similarity between the two-dimensional weight matrix and a weight matrix in a pre-constructed database by adopting an Euclidean distance judgment analysis method, and determining the pollution source. According to the method, the active components in the water quality are enhanced by electrochemical disturbance, more information is provided for water pollution source analysis to excavate hidden water quality spectral information, and the identification degree of water quality detection traceability is increased; and meanwhile, pollution source tracing analysis is performed by using an entropy weight method-Euclidean distance, so that the accuracy of pollution source identification and analysis is improved.