399results about How to "Avoid recontamination" patented technology

The invention relates to a primer middle sequence interference PCR (Polymerase Chain Reaction) technology. The improved PCR technology is characterized in that one segment of relatively non-complemented or same-sequence basic group primer molecules in the intermediate domain of primers perform the antisense interference inside and outside so as to competitively destroy the polymerization among the primers to selectively inhibit the primer dimer (PD) from being amplified. For the interference of the intermediate domain of the primers, based on the primers optimally selected by the conventional design principle, the technology that the intermediate domain (ID) of a pair of the primers are in parallel but are not complemented with each other or are in the same sequence or/and the technology that ID antisense oligonucleotides (Oligo) are added into the primers to perform the interference action or/and the Oligo antonymy is carried out in the primer molecules via the ID so as to perform the interference action are adopted, or the combined technology of the three types of the technologies is adopted. As a result, only the ID of the primers is interfered while the target specific amplification is not influenced; the combining force acting on the minority of base-group pairing hydrogen bonds at the tail end and the base-group hydrogen bonds outside the tail end due to the action of the primers is dispersed to a maximum extent, so that the PD is selectively inhibited. Therefore, the PD accumulation in the PCR system is avoided. If the mineral oil is additionally used, the sealed primers can slowly release the hot starting and the UDG pretreatment so as to prevent aerosol glue as a byproduct of the PCR system from causing the pollution. Consequently, the nucleic acid is amplified reliably and the real-time fluorescence PCR is quantified accurately.

The invention discloses a cleaning machine with a supporting and thrusting mechanism, which comprises a rack and a plurality of cleaning tanks, wherein a lifting bracket used for supporting material baskets is arranged in each cleaning tank; at least one side edge of each lifting bracket is connected with a lifting cross beam through a bracket connecting rod; a lifting driving mechanism mounted on the rack can drive the lifting brackets to be lifted to and fro in the vertical direction through the lifting cross beam; horizontal material basket push rods used for horizontally pushing the material baskets to move are further arranged above the cleaning tanks and are driven by a horizontal driving mechanism; fixed brackets are fixedly arranged between adjacent cleaning tanks; upper bearing surfaces of the fixed brackets are positioned in the same bearing plane as bearing surfaces of the lifting brackets lifted to high positions; and the bearing plane is higher than tank openings of the cleaning tanks. The cleaning machine has the advantages of stability and reasonableness in structure and great carrying capacity; and particularly, a cleaning and conveying path of the cleaning machine is reasonable, the effective cleaning time is long and the work efficiency is high.

The invention provides a multifunctional nanometer filter material and a preparation method; the multifunctional nanometer filter material consists of nanometer fibres and is characterized in that: according to the weight percentage, the surface and interior of the nanometer fibre comprises 3wt-10wt% of nanometer silver oxide, 0.5wt-5wt% of nanometer silicon oxide, 3wt-10wt% of nanometer titanium oxide, 0.3wt-3wt% of nanometer zinc oxide and 3wt-15wt% of nanometer active carbon. The preparation method of the invention comprises the steps as follows: polymer solution with the Berry number of 1-2 is formed firstly by mixing; subsequently, ultrasonic dispersing, static spinning, freezing, drying and carbonizing are carried out so as to gain the multifunctional nanometer filter material. The multifunctional nanometer filter material prepared by the method of the invention has the effects of effectively filtrating and blocking particulates and killing the micro-organism at the same time, and can keep the self-cleaning of the micro-organism.

A buckwheat leafbud tea is prepared from the leaf of buckwheat, the leafbud of buckwheat, or their mixture. Its preparing process is also disclosed.

The invention relates to a material for purifying and processing drinking water, which is made of high-temperature sintering tourmaline nanometer powder, alkali mine powder, metal magnesium and the like. The invention is characterized in that the material for purifying drinking water contains 5 to 30 weight percentages of sea shell powder, 10 to 40 weight percentages of tourmaline nanometer powder, 10 to 40 weight percentages of alkali mine powder and 2 to 20 weight percentages of metal magnesium. The invention has convenient utilization and low price, can effectively remove the foreign odor in water, has the functions of resisting bacteria and mineralizing, activating and minifying water molecular groups of the drinking water and enables the human body to achieve pH value balance.

The invention discloses an aluminum sheet dedusting and conveying roller comprising driving roller, driven roller, a supporting frame, a dust collector, a ventilation hood, adjusting hanging rods, a roller brush and a drive motor. The motor is arranged at the bottom of the supporting frame. The driving rollers are symmetrically arranged on columns on two sides of the supporting fame, respectively. The driven rollers are symmetrically arranged above the driving rollers respectively. The roller brush is arranged between the driven rollers on two sides thereof. The adjusting hanging rods are arranged at two ends of the roller brush, respectively. An air channel is arranged between the top of the ventilation hook and the dust collector. According to the arrangement, the driving rollers are used with the driven rollers to convey the aluminum sheets, and meanwhile, dusts and foreign materials on the surface of the aluminum sheet are raised by the roller brush and collected by the ventilation hook and the dust collector, thus secondary pollution is avoided, thorough cleaning is achieved, and spray lacquer surface quality can be improved.

The invention relates to the field of energy, in particular to a method for producing biogas by taking Entermorpha as a material. The method comprises the following steps: (1) the Entermorpha is rinsed by water; (2) the Entermorpha obtained in step(1) is crushed until the grain size of the Entermorpha is between 0.5 and 2mm; (3) the Entermorpha obtained in step(2) is placed into a fermentation device and added with inoculum, and the weight ratio of the inoculum TS(total solid content) to the Entermorpha TS(total solid content) is between 1 to1 and 1 to 5; (4) the fermentation device is added with fresh water, and the total solid content in the fermentation device is adjusted to between 2.5 and 10 percent; (5) alkali is added into the fermentation device, and the initial pH value of the mixture obtained in step(4) is controlled to between 6.5 and 7.5; and (6) the fermentation device is sealed and placed in the thermostatic water bath, and the fermentation temperature is controlled to between 32 and 38 DEG C. The method has the advantages of realizing the resource utilization of the Entermorpha, having simple process and easy operation, improving the marine environment and producing the energy product of biogas.

The invention discloses a glass cleaning device, and particularly relates to the technical field of cleaning. The glass cleaning device comprises a first support and a second support. The first support and the second support are arranged parallelly in the up-down direction, a plurality of upper rotating shafts and lower rotating shafts are arranged in the vertical directions of the first support and the second support, and the upper rotating shafts are opposite to the lower rotating shafts in an up-down mode; one ends of the upper rotating shafts are rotatably connected with the first support, one ends of the lower rotating shafts are rotatably connected with the second support, and disc brushes are arranged at the other ends of the upper rotating shafts and the lower rotating shafts correspondingly; and a lifting unit capable of enabling the first support to extend and retract in the vertical direction is arranged at one end of the second support, and sliding units which are matched with each other are arranged at the other end of the second support, wherein the other end of the second support is opposite to the lifting unit. According to the glass cleaning device, the two faces of glass can be cleaned simultaneously, the cleaning time is shortened, and glass cleaning is more thoroughly; and the situation that when the glass is turned over manually, the glass is polluted again is avoided, the labor cost is saved, and the efficiency is improved.

The invention discloses a preparation method for high-toughness monofilament blended cotton yarn. The preparation method comprises the following steps of carrying out cotton feeding, rolling, cotton carding and drawing to obtain rough yarn after carrying out cotton opening and scutching on long staple cotton, fine staple cotton, prefabricated monofilament materials and mixed cotton, and obtaining the high-toughness monofilament blended cotton yarn through spinning and spooling, wherein the prefabricated monofilament materials are obtained through draining and drying after carrying out primary boiling and dehydrating, primary steaming, secondary boiling and dehydrating, secondary steaming, standing, bleaching and washing. The high-toughness monofilament blended cotton yarn prepared by the preparation method has the characteristics of high toughness, low cost and the like, meanwhile, the air permeability is good, and the comfort is strong.

The invention relates to the field of lithium ion battery production equipment, in particular to a dust removal device of a pole piece of a laminated battery, which comprises a conveyor belt, an upper brush disposed above the conveyor belt and a lower brush disposed below the conveyor belt. The dust removal device of the pole piece of the laminated battery is characterized in that a compression roller matched with the conveyor belt is arranged above the conveyor belt, the upper brush and the lower brush are correspondingly arranged above and below the conveyor belt, and a dust collection port connected with a dust collector pipeline is disposed below the matching position of the upper brush and the lower brush. The dust removal device of the pole piece of the laminated battery has the advantages of being simple in structure, low in energy consumption and good in dust removal effect and uses less parts and the like.