The invention relates to an accurately-metering nondestructive automatic ink injection machine. The ink injection machine comprises a shell, a vacuum pump arranged in the shell, a negative pressure main valve, a waste liquid tank, a control module, and at least one set of ink injection units, wherein the vacuum pump is connected with the waste liquid tank through the negative pressure main valve; and the waste liquid tank is connected with each set of ink injection units. The automatic ink injection machine is suitable for injecting ink to various ink boxes with a structure such as an ink box and sprayer head integrated structure, an ink box and sprayer head separated structure, an in-box sponge structure, an in-box non-sponge structure, and the like, which means the automatic ink injection machine can inject ink to all most ink boxes on the market; thus, the automatic ink injection machine has a wide application range. When the ink is injected, the sponge structure is protected from being damaged and the sprayer heat from being worn, and the regeneration times of the ink box can be increased to a maximum extent to save the cost of a user. When the ink injection is finished, the ink can be fully filled to an ink spray nozzle, the automatic ink injection machine can be directly arranged on a printer for use and the ink does not need to be absorbed to the ink spray nozzle from the sprayer head with negative pressure.