33results about How to "Avoid ethical issues" patented technology

The invention relates to a nerve stem cell injection made from retina pigment epithelial cell, to treat paralysis agitans, wherein said injection at least comprises 3*106 nerve stem cells of human hRPE, while said nerve stem cells are cultivated from retina pigment epithelial cells. And its production comprises that: (1), separating and cultivating cells, that separating the hRPE cells, mixing uniformly to prepare single cell suspension; (2), hRPE cell cultivation and generation that digests original cultivation liquid, ending digestion, blowing and beating to form cell suspension, eccentrically filtering and removing the upper clear liquid, adding cultivating substrate, to disperse and form cell suspension, filtering with cell screen, transferring cell into cultivating bottle, generating to at least fifth generation; (3), adding common salt into hRPE nerve stem cell, to prepare cell suspension injection.

The invention belongs to the field of microfluidic technology and biotechnology, and relates to a multifunctional organ chip based on a microfluidic technology, and a preparation method and an application thereof. The multifunctional organ chip based on the microfluidic technology comprises a substrate, the substrate at least comprises two structural layers, each structural layer is provided witha micro-channel and a cell culture area communicated with the micro-channel, the cell culture areas of every two adjacent structural layers are communicated, and a porous membrane is arranged at the communication position. The invention provides the multifunctional organ chip based on the microfluidic technology, which not only can stably culture human corneal cells in a culture area to form three-dimensional corneal tissues, but also can detect the integrity of an established corneal barrier, and solves the problem that in-vitro two-dimensional culture and in-vivo animal experiments are difficult to correctly reflect human corneas, and the requirements of scientific research and clinical application on an in-vitro bionic research model are met.

The invention belongs to the technical field of food, and particularly relates to printable protein network double gel which comprises an oil phase and a water phase, the oil phase comprises edible oil added with an oil gelling agent; the aqueous phase comprises a salt solution to which vegetable protein and a hydrogel are added. The invention provides a method for preparing double gel which is stable for a long time, can be used for 3D printing and is high in printing accuracy through an emulsion template method, vegetable protein and hydrogel are added into a salt solution to form a mixed solution, edible oil containing oil gel is added, the mixed solution is emulsified through high-speed shearing in a water bath, cooling is performed to enable the mixed solution to be fully gelatinized, and then the double gel is prepared. The prepared oil-in-water double gel has higher stability and better deformation resistance, the preparation process is simple, industrialization is easy to realize, and the raw materials are green, healthy and economical; the method can be used for simulating adipose tissues and connective tissues in artificial meat, avoids saturated fatty acids and trans-fatty acids, and better simulates the sense organ and composition of animal meat.

The invention provides an organ perfusion teaching and training system. The system comprises an organ compartment for holding an organism organ (1) and affiliated blood vessels; a liquid storage compartment (3) located below the organ compartment (2) and used for storing circular perfusion liquid; a temperature maintaining device (4) for maintaining the temperature of the organism organ; a perfusion pipeline (8) for connecting the liquid storage compartment (3) and the affiliated blood vessels of the organism organ (1), and performing pressurizing and circular perfusion on the organism organ (1) and the affiliated blood vessels under the driving of a pump (7); the pump (7) for pressurizing the circular pipeline (8), and performing pressurizing and circular perfusion on the organism organ (1) and the affiliated blood vessels; a display (35) for displaying an operation video; a base (61) for supporting, moving and lifting the entire device; and an organ perfusion preservation device capable of perfusing single or multiple organism organs (1) at the same time. Multiple organs can be perfused at the same time through the system provided by the invention.

The invention relates to the field of biomedicine, in particular to a menstrual blood source stem cell preparation and a preparation method and application thereof. The menstrual blood source stem cell preparation comprises menstrual blood source stem cells, albumin and normal saline. The experiment indicates that the menstrual blood source stem cell preparation can keep the survival rate of the menstrual blood source stem cells at 99% or above, the cells are uniform in size, and the cells are each in a shuttle shape. After being preserved for 1 month at the temperature of 0-4 DEG C, the cells are still kept in good forms, the abnormal phenomenon such as increase of the size does not appear, the survival rate is still kept at 95% or above, and through surface antigen detection, the menstrual blood source stem cells still keep the characteristics of stem cells, no differentiation is generated, and good activity is achieved. The menstrual blood source stem cell preparation can achieve a good treatment effect when used for treating premature ovarian failure, and after the menstrual blood source stem cell preparation is used for a week, the number of follicles of a mouse is obviously increased.

The invention discloses a dental pulp stem cell exosome preparation as well as a preparation method and application thereof, and relates to the technical field of biology. Dental pulp stem cells can express neuronal cell surface markers, can be differentiated to nerve cells and promote the proliferation of the nerve cells, so that the dental pulp stem cells can be used for preparing cell preparations for repairing nerve injuries such as traumatic brain injuries (TBI). The dental pulp stem cells are used for replacing traditional embryonic stem cells, neural stem cells or bone marrow stem cellsto be used for treating the nerve injuries, and have the advantages of being rich in source, simple and convenient to obtain, low in immunogenicity and capable of avoiding ethical problems. Dental pulp stem cell exosomes can accurately target recipient cells and are used for preparing nerve repair preparations for the injuries such as the TBI, and then treatment of the nerve injuries such as theTBI by the exosomes is achieved. The dental pulp stem cell exosomes can also be used for preparing neuroinflammation inhibitors.

The invention relates to the application of umbilical cord Huatong jelly mesenchymal stem cells in the preparation of medical biological materials. This medical biomaterial can be used for human intervertebral disc cell transplantation, and can also be used as seed cells for intervertebral disc tissue engineering. It uses fresh human umbilical cord as material to prepare Huatong jelly mesenchymal stem cells, and uses the intervertebral disc specimen removed during intervertebral discectomy as material to isolate and culture normal nucleus pulposus cells; then apply non-contact or contact cell co-culture method to induce Differentiation of umbilical cord Huatong jelly mesenchymal stem cells.

The invention discloses a cardiac electrophysiological signal simulation system and a simulation method thereof. The system comprises a transparent cardiac chamber model, a catheter device for a cardiac minimally invasive interventional surgery, a locating system for locating the position of a catheter head and an upper computer containing an electrocardiogram signal upper computer translation program, wherein the cardiac chamber model is connected to the upper computer by virtue of the locating system; the cardiac chamber model is additionally connected to the catheter device; and in accordance with electrocardiogram signals which are pre-saved in the upper computer as well as preset binding relation characteristics in a heart, electrocardiogram signals, which are in correspondence to the cardiac chamber model and a touch point of the catheter head in the catheter device, are translated and displayed on the upper computer by virtue of the electrocardiogram signal upper computer translation program. With the implementation of the simulation system and the simulation method disclosed by the invention, a blank between theoretical training and animal experiments in a cardiac interventional surgery is filled; and on the basis of safe, radiation-free, transparent and intuitive operating experiences, real feedback hand feeling as well as real-time and accurate electrocardiogram signal feedback, the training efficiency of doctors is improve, so that more experienced doctors can be provided within a unit time.

The invention discloses a device and method for gender identification of an egg embryo based on heart rate measurement. The device comprises a transmission module, a laser module, an eggshell pick andplace module, a light source module, an image acquisition module, a control module and a computer processing terminal, the control module is respectively connected to the laser module, the eggshell pick and place module and the computer processing terminal, the laser module, the eggshell pick and place module and the image acquisition module are all located above the transmission module, and aresequentially disposed in the moving direction of the transmission module, and the light source module is located between the image acquisition module and the transmission module. The device utilizes the heart rate value of an egg hatching stage to judge the gender of eggs, is beneficial to rationally treating the eggs of different genders according to the production needs, not only effectively improves the production efficiency of the chicken industry, but also avoids killing of a large number of zero-aged cocks, avoids ethical issues, is convenient to operate, and is conducive to the actual production environment.

The invention discloses an endometrium stem cell exosome gel, which comprises traditional Chinese medicine ingredients and endometrium stem cell exosome, wherein the traditional Chinese medicine ingredients comprises ootheca mantidis, ossa sepiae, fructus alpiniae oxyphyllae, radix linderae, cnidii fructus, endoconcha sepiae, aloe, stigma croci and rose hydrosols. The endometrium stem cell exosome gel adopts the traditional Chinese medicine ingredients which have higher safety than Western medicines, the traditional Chinese medicines are purely natural, are free from pollution, have the functions of diminishing inflammation and inhibiting bacteria, can improve female health, reduce female gynecological diseases, repair female uterus injuries and recover vaginal elasticity. The endometrium stem cell exosome gel is conveniently used and has good absorbency, the water-soluble gel has good absorbency, and the molecules of the exosome are small so as to be more favorable for absorption; and the endometrium exosome has a good combination effect with the traditional Chinese medicines, carries out convergence and firming on the endometrium, belongs to autologous stem cells and avoids ethical issues, and the autologous stem cells are not be rejected.

The invention discloses a method for evaluating granular chemicals released in consumer goods by simulating a lung tissue liquid in-vitro substitution technology and relates to the technical field of consumer goods pollutant exposure simulation detection. According to the method, on the basis of simulated lung fluid, an in-vitro simulation device and a Tenax structure are used for carrying out an absorption test on pollutants in the simulated lung fluid, and a test result is beneficial for evaluating inhalation bioaccessibility of a pollutant particulate matter sample and is beneficial for analyzing the concentration level and composition characteristics of the pollutants migrated into the simulated lung fluid. The dialysis bag is used in the simulation process, the situation that the added particulate matter enters the Tenax structure and is difficult to separate from the Tenax structure, and consequently bioaccessibility is overestimated can be prevented, therefore, the bioavailability migration volume of the particulate target object released by the consumer goods through respiration can be evaluated more accurately, the evaluation result provides data for safety of pollutants, and a more scientific basis is provided for scientific limitation of pollutants in consumer goods and establishment of corresponding standards.

A drug screening against Parkinson's Disease and the construction of assessment platform are disclosed. The platform is supported by gene cloning technology and transfer gene technology, in vitro analog affection Baramine neuron can be used to screen provisional compound against Parkinson's Disease and leader compound. It can be used to make research for pharmacological assessment and penetrating mechanism.

The method of preparing nucleus embryo stem cell by means of somatic nucleus transplanting technology is one method of somatic nucleus transplanting process to prepare cell. Under microphone operation instrument, nerve nucleus of adult animal is transplanted into denucleated embryo stem cell cytoplast to form embryo stem cell with completely new genome, and the new embryo stem cell is cultured to divide and proliferate to 28 pieces. Embryo stem cell and nerve cell are homologous cells and the prepared cell is nucleus transplanted embryo stem cell with the genome of donor cell. The present invention solves serial problems in somatic nucleus transplanting and can promote the development of cell engineering and tissue engineering relevant to human embryo stem cell.

The present invention features methods and compositions for treating a patient who has a neurological deficit. The method can be carried out, for example, by contacting (in vivo or in culture) a neural progenitor cell of the patient's central nervous system (CNS) with a polypeptide that binds the epidermal growth factor (EGF) receptor and directing progeny of the proliferating progenitor cells to migrate en masse to a region of the CNS in which they will reside and function in a manner sufficient to reduce the neurological deficit. The method may include a further step in which the progeny of the neural precursor cells are contacted with a compound that stimulates differentiation.

The invention belongs to the technical field of cartilage repair, and discloses a hydrogel material for promoting cartilage repair as well as a preparation method and application thereof. The preparation method comprises the following steps: pretreating BMSC (bone mesenchymal stem cells) by adopting TGF-beta3 to obtain TGF-beta3-miRNA-extracellular vesicles, co-culturing the BMSC and the TGF-beta3-miRNA-extracellular vesicles to obtain the BMSC of the TGF-beta3-miRNA-extracellular vesicles, and uniformly mixing the obtained product with hydrogel with biocompatibility to obtain the hydrogel material for promoting cartilage repair. According to the material, due to the synergistic effect of TGF-beta3-miRNA-extracellular vesicles, the limitation of promoting cartilage differentiation of BMSC by only using TGF-beta3 is overcome, the cartilage differentiation efficiency of BMSC is improved, and the material has a remarkable and excellent cartilage repair effect and can be applied to treatment of damaged cartilage.

The invention relates to the technical field of tissue engineering, in particular to a Schwarm progenitor cell derived from a marrow neural crest cell and application of the Schwarm progenitor cell to promotion of nerve regeneration. The rat marrow-derived neural crest cell N1, namely, BM-NCC clone N1, is provided, the preservation number is CCTCC NO:C201647, and the rat marrow-derived neural crest cell N1 has the performance of the Schwarm progenitor cell (SCP). The invention further provides a subculture method of the BM-NCC clone N1 and a method for induced directional differentiation to the Schwarm cell (SC). The BM-NCC clone N1 is expected to be applied to nerve regeneration promotion therapy based on cells.

The invention discloses a skin chip for gas-liquid phase culture. The skin chip comprises an upper plate, a middle plate, a porous membrane and a bottom plate, which are successively arranged up and down. The bottom surface of the upper plate is provided with a gas channel which is concave upward. The skin chip also comprises a gas inlet and a gas outlet which are arranged on the skin chip. The gas inlet and the gas outlet are respectively led to two ends of the gas channel. A through groove penetrates through the middle plate. The through groove is located below the gas channel. The porous membrane is located below the through groove and covers the bottom of the through groove. The upper surface of the bottom plate is provided with a culture medium channel which is concave downward. The culture medium channel is located below the through groove and is communicated with the through groove through the porous membrane. The skin chip also comprises a culture medium inlet and a culture medium outlet which are arranged on the skin chip. The culture medium inlet and the culture medium outlet are respectively led to two ends of the culture medium channel. The invention has the following advantages: nutrients can be provided in time and metabolic waste can be removed timely; there is no need to replace fresh culture mediums frequently; and manpower and material resources are saved in the culture process.

The invention relates to a method for inducing minimal embryonic stem cells to differentiate into cardiomyocytes. The method includes the following steps: (1) obtaining CD45-Lin-CD106+cells (minimal embryonic stem cells) in rat bone marrow through flow cytometry sorting; (2) inducing, by 5-azacytidine, the minimal embryonic stem cells to differentiate; and (3) through qRT-PCR and immunofluorescence, detecting myocardial protein related genes, and validating the process that the 5-azacytidine induces the minimal embryonic stem cells to differentiate. The purpose of the method is to isolate andculture the minimal embryonic stem cells from rat bone marrow tissue; through the adoption of the 5-azacytidine to induce and differentiate, biological characteristics and directed differentiation characteristics can be observed, so that a convenient and fast approach for cell transplantation treatment of clinical bradyarrhythmia can be found. Validation results are reasonable and accurate, and experimental processes are precise and cautious.

The invention relates to the technical field of tissue engineering, in particular to a Schwarm progenitor cell derived from a marrow neural crest cell and application of the Schwarm progenitor cell to promotion of nerve regeneration. The rat marrow-derived neural crest cell N1, namely, BM-NCC clone N1, is provided, the preservation number is CCTCC NO:C201647, and the rat marrow-derived neural crest cell N1 has the performance of the Schwarm progenitor cell (SCP). The invention further provides a subculture method of the BM-NCC clone N1 and a method for induced directional differentiation to the Schwarm cell (SC). The BM-NCC clone N1 is expected to be applied to nerve regeneration promotion therapy based on cells.

The invention relates to a nerve stem cell injection made from retina pigment epithelial cell, to treat paralysis agitans, wherein said injection at least comprises 3*106 nerve stem cells of human hRPE, while said nerve stem cells are cultivated from retina pigment epithelial cells. And its production comprises that: (1), separating and cultivating cells, that separating the hRPE cells, mixing uniformly to prepare single cell suspension; (2), hRPE cell cultivation and generation that digests original cultivation liquid, ending digestion, blowing and beating to form cell suspension, eccentrically filtering and removing the upper clear liquid, adding cultivating substrate, to disperse and form cell suspension, filtering with cell screen, transferring cell into cultivating bottle, generatingto at least fifth generation; (3), adding common salt into hRPE nerve stem cell, to prepare cell suspension injection.

The invention relates to a schizophrenia classification method and system based on a multi-center model, and the method comprises the steps: 1, data preparation: collecting a brain MRI image of a sample, processing the brain MRI image, and extracting a plurality of brain structure features to obtain a feature matrix; carrying out covariant regression processing on the feature matrix, and then carrying out standardization processing; enabling each center to prepare a respective data set according to the step; 2, enabling each center to construct a respective single-center model by using a machine learning classifier, and training the respective single-center model by using the respective data set; 3, classifying to-be-classified test samples by using each single center model to obtain classification probability values of the to-be-classified test samples corresponding to each center; and performing weighted summation on the classification probability value and the weight of each center to obtain a classification probability value based on a multi-center model, and integrating all the single-center models into the multi-center model for classification. Data sharing of each center is realized, and each center does not need to share original data.

The invention provides a method for screening a diabetic nephropathy drug in vitro by determining the change of glucose concentration in culture solution of a unit cell of glomerular mesangial cells, renal tubular epithelial cells and endothelial cells after the drug is administrated and the change of the cellular proliferation inhibition rate. The method is not only suitable for screening of small molecule compounds, but also especially suitable for screening of prescription compatibility of traditional Chinese medicine compositions and screening of preparation processes. When a model and the method are applied to screening of the drug, the cost is low, the time is short, and the operation is simple, convenient and easy and is strong in reliability, therefore, the method has the important significance for early screening of drug candidates for diabetic nephropathy.

The invention discloses a transforming growth factor beta 1 active polypeptide and application thereof, and belongs to the technical field of transforming growth factor beta 1 active polypeptides. The amino acid sequence of the transforming growth factor beta 1 active polypeptide is as shown in SEQ ID NO. 23. The invention also discloses application of the transforming growth factor beta1 active polypeptide in preparation of drugs for wound healing. According to the invention, a phage random dodecapeptide library is used for screening a TGF-beta1 key sequence, in-vitro synthesis of the TGF-beta1 active polypeptide is carried out, a large amount of TGF-beta1 active polypeptide can be synthesized in a short time, and the production cost is greatly reduced.

The invention discloses a semi-in-vivo animal experiment blood compatibility detecting system and application thereof. The detecting system comprises a heating device, a vessel, hoses, a pressure sensor and a differential pressure flow sensor, wherein the first hose, a bioengineering material, the second hose and an animal form a blood circulation loop. The semi-in-vivo animal experiment blood compatibility detecting system can control the experimental temperature at a stable value in real time and monitor the blood pressure and flow rate in the bioengineering material in real time, thereby better evaluating the vascular patency and blood compatibility.

The invention discloses a keratinocyte growth factor active polypeptide and application thereof, and belongs to the technical field of keratinocyte growth factor active polypeptides. The nucleotide sequence of the keratinocyte growth factor active polypeptide is as shown in SEQ ID NO.54, and the amino acid sequence of the keratinocyte growth factor active polypeptide is as shown in SEQ ID NO.55. The invention also discloses an application of the keratinocyte growth factor active polypeptide in preparation of drugs for wound healing. The KGF gene sequence is obtained by screening a KGF key sequence by applying a phage random polypeptide library, and KGF active polypeptide can be directly synthesized in vitro and can be massively synthesized in a short time, so that the production cost is greatly reduced.

The invention provides a culture medium and a method for inducing airway basal stem cells to differentiate into bronchoalveolar stem cells, and relates to the technical field of stem cell induced differentiation. The culture medium comprises a primary induced differentiation culture medium and a secondary induced differentiation culture medium; the primary induced differentiation culture medium comprises the following components: a fibroblast growth factor 10, a keratinocyte growth factor and a glycogen synthase kinase-3 inhibitor; the secondary induced differentiation culture medium is prepared from the following components: a fibroblast growth factor 10, a keratinocyte growth factor, a keratinocyte growth factor, a gamma secretase inhibitor and a phosphodiesterase inhibitor. According to the method disclosed by the invention, the BC is subjected to induction culture by adopting the induction culture medium and can be efficiently converted into the BASC. According to the culture medium and the culture method, autologous airway basal stem cells can be successfully induced into bronchoalveolar stem cells, and the induction conversion rate is high.

The invention provides a method for in vitro screening of drugs for diabetic nephropathy by measuring the change of glucose concentration and the change of cell proliferation inhibition rate in the culture solution of unit cells of mesangial cells, renal tubular epithelial cells and endothelial cells after administration. method. The method is not only suitable for the screening of small molecular compounds, but also particularly suitable for the screening of prescription compatibility and preparation process screening of traditional Chinese medicine compositions. Applying this model and method to screen drugs is low in cost, short in time, easy to implement, and highly reliable, and is of great significance for the early screening of candidate drugs for diabetic nephropathy.

The invention provides a treatment method and a treatment agent for a biological specimen. According to the treatment method provided by the invention, before a biological specimen is in contact witha cross-linking agent, the surface of the biological specimen is coated with gelatin to form a gelatin coating layer. The treatment method comprises the following main steps: 1, wrapping a biologicalspecimen with heated gelatin hydrogel; and 2, cooling and solidifying the gelatin hydrogel, and then placing the biological specimen coated with the gelatin in a cross-linking agent for cross-linkingtreatment so as to enable the gelatin coating layer to form a fixed insoluble cross-linked product. The biological specimen treating agent comprises gelatin, and the concentration of the gelatin ranges from 15% to 40%. The treatment method and the treatment agent for the biological specimen have the following beneficial effects: (1) pain and trauma of a patient are reduced; (2) avoiding of ethicalproblems; and (3) large-scale research becomes possible.

The invention relates to a rapid detection kit for diarrhea shellfish toxin and a detection method thereof. Belonging to the field of aquatic product safety testing, the kit includes: (1) 96-well microwell plates embedded with protein phosphatase 2A (PP2A) enzyme gel; (2) okadaic acid concentration gradient series standard solutions; (3) Buffer solution A; (4) Buffer solution B; (5) Substrate chromogenic solution; (6) Quality control sample solution. The invention adopts improved sol-gel technology to immobilize PP2A enzyme on the bottom of microporous plate, and utilizes diarrhea shellfish toxin to inhibit PP2A enzyme from catalyzing the dephosphorylation of colorless substrate disodium p-nitrophenyl phosphate under alkaline conditions. The yellow product reacts to nitrophenol (pNP), and the toxin is quantitatively detected according to the dose-response relationship between the absorbance of pNP at 405nm and the concentration of the toxin. The invention has simple sample pretreatment and detection steps, low operational technical requirements, short time consumption, low cost and high accuracy, and is suitable for rapid screening of diarrheal shellfish toxins in large batches of shellfish samples.

The invention discloses a method for inducing differentiation of human adipose-derived stem cells into neural-like stem cells through co-cultured with umbilical cord mesenchymal stem cells and cell factors, and the neuron-like stem cells can be provided for medical institutions to treat nerve cell deficiency diseases such as child cerebral palsy and the like. According to the method, ethical problems and insecurity of allogeneic cell transplantation are avoided, the separated and purified human adipose-derived stem cells are transformed, neural stem cells which are consistent in purity, non-gene-edited, safe, stable and suitable for large-scale culture can be obtained, the neural stem cells are suitable for individualized treatment of the cerebral palsy, and a stable and feasible method can be provided for clinical treatment of the cerebral palsy.