Skin chip for gas-liquid phase culture

Abstract

The invention discloses a skin chip for gas-liquid phase culture. The skin chip comprises an upper plate, a middle plate, a porous membrane and a bottom plate, which are successively arranged up and down. The bottom surface of the upper plate is provided with a gas channel which is concave upward. The skin chip also comprises a gas inlet and a gas outlet which are arranged on the skin chip. The gas inlet and the gas outlet are respectively led to two ends of the gas channel. A through groove penetrates through the middle plate. The through groove is located below the gas channel. The porous membrane is located below the through groove and covers the bottom of the through groove. The upper surface of the bottom plate is provided with a culture medium channel which is concave downward. The culture medium channel is located below the through groove and is communicated with the through groove through the porous membrane. The skin chip also comprises a culture medium inlet and a culture medium outlet which are arranged on the skin chip. The culture medium inlet and the culture medium outlet are respectively led to two ends of the culture medium channel. The invention has the following advantages: nutrients can be provided in time and metabolic waste can be removed timely; there is no need to replace fresh culture mediums frequently; and manpower and material resources are saved in the culture process.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a skin chip cultured in gas-liquid phase. Background technique [0002] The skin is the largest organ in the human body and serves as a physiological barrier around the muscles and bones to protect tissues and organs in the body. However, the skin is constantly exposed to numerous biochemical agents (eg, cosmetics, skin washes, etc.), ultraviolet rays, pathogens, environmental pollutants, and microorganisms. A rapid increase of these factors can cause various skin reactions such as skin inflammation, irritation, allergy and even skin cancer. Furthermore, the extreme loss of skin function and structure due to injury and disease leads to severe physiological imbalances that may further lead to disability or even death. Therefore, the construction of bionic skin in vitro is of great significance for the diagnosis and treatment of skin diseases and the provision of transplantable ...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention lies in: the skin tissue culture in the prior art cannot provide nutrients and remove metabolic waste in time, requires frequent replacement of fresh medium, and the technical problems of manpower and material resources in the culture process

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