The invention discloses preparation of a nano-composite bactericidal and antiviral material and an application thereof, and belongs to the technical field of antivirus. The material is prepared from the following components: nano-silver particles, nano-cuprous oxide particles, a traditional Chinese medicine extracting solution, polymer nano-particles, a stable dispersing agent, a humectant and water. The material has the characteristics of no toxicity, harmlessness, environmental protection, strong durability, broad-spectrum / efficient sterilization, virus resistance and the like, is convenientand simple to use, can be directly sprayed, can quickly kill various bacteria, viruses and the like in various outdoor and indoor spaces and articles, and inhibits the spreading problem of epidemic viruses confronted by human beings at present.