37 results about "Viral nucleocapsid" patented technology

The invention relates to the field of biological medicine, in particular to an SARS-CoV-2 detection kit based on an indirect method. The kit comprises (a) a solid phase conjugate. The solid-phase conjugate consists of a solid-phase carrier as well as an antigen and an antibody which coat the solid-phase carrier. The antibody is a monoclonal antibody for resisting novel coronavirus nucleocapsid N protein. The antigen comprises an S1 subunit, an S2 subunit, an RBD, an N protein and an E protein of the SAR-CoV-2 Spike protein. The kit further comprises (b) an anti-antibody and an antibody marked with a chemiluminescent marker. The antibody is a monoclonal antibody to the novel coronavirus nucleocapsid N protein, but is bound to a different epitope from the antibody in (a). The anti-antibody is at least one of an anti-human IgG secondary antibody, an anti-human IgA secondary antibody and an anti-human IgM secondary antibody. The anti-antibody is a monoclonal antibody or a polyclonal antibody.

The invention discloses a standard positive reference material for rana grylio virus (RGV) detection. In the invention, a pair of primers are firstly designed, which can specifically amplify capsid protein complete genes of soft-shelled turtle iridovirus nuclears, wherein the nucleotide sequences of the primers are shown in SEQ ID.1 and SEQ ID NO.2; the capsid protein complete genes of the soft-shelled turtle iridovirus nuclears amplified by the primers have the nucleotide sequence shown in SEQ ID NO.3; and a recombinant plasmid pGEM-T-S containing the capsid protein complete genes of the soft-shelled turtle iridovirus nuclears is constructed and screened after a proper PCR reaction system and reaction conditions are found out, which can be used as the standard positive reference material for molecular biology detection on STIV MCP, EHNV MCP and TFV MCP.

The invention provides a method for preparing a polyclonal antibody to rice stripe virus nucleocapsid protein and its application, and belongs to the technical field of rice stripe virus detection. The polyclonal antibody to rice stripe virus nucleocapsid protein CP is based on rice stripe virus The nucleocapsid protein CP is obtained by immunizing animals with an immunogen; the amino acid sequence of the rice stripe virus nucleocapsid protein CP is shown in SEQ ID No.1. The polyclonal antibody of the rice stripe virus nucleocapsid protein of the present invention has the characteristics of low cost, wide application, multiple audiences, high sensitivity, etc., and can be used in immunospot assay, enzyme-linked immunosorbent assay, western blot, immuno A number of biological detection experiments such as fluorescence method and immunoelectron microscope method. Using the polyclonal antibody of the present invention, the early warning of the disease is carried out by the striatellus striatellus at the beginning of the outbreak of the rice stripe leaf blight, which is beneficial to the multi-means disease monitoring.

A method for preparing a precious metal palladium nanorod by means of cotton bollworm baculovirus nucleocapsid mainly includes the steps that cotton bollworm karyotype polyhedron virus raw powder is dispersed through ultra-pure water to obtain grey polyhedrin suspension, alkaline hydrolysis liquid is added into the grey polyhedrin suspension, the mixture stands still for 15-25 min, then the pH value is adjusted to be 7 through acetic acid, chloroform is added, centrifugalization is carried out for 5-10 min at the speed of 5000-6000 r / min, supernatant fluid is sucked, centrifugalization is carried out for 1-2 h at the temperature of 4-10 DEG C and at the speed of 25000-30000 r / min, pigmentation is re-suspeneded through a PBS solution, and a cotton bollworm baculovirus nucleocapsid solution is obtained; a PdCl2 solution is added into the cotton bollworm baculovirus nucleocapsid solution and fully and evenly mixed, incubation is carried out in a shaking mode for 15-25 h at the temperature of 20-30 DEG C and at the speed of 130-170 r / min, and the precious metal palladium nanorod loaded by the cotton bollworm baculovirus nucleocapsid is obtained. The method is simple in process, environmentally friendly and efficient, industrial production is easy to achieve, and the prepared palladium nanorod is stable in appearance and uniform in size.

The invention relates to the assembling field of virus nucleocapsid, particularly to a baculovirus nucleocapsid assembling necessary element and an application thereof in nucleocapsid assembling, establishment of a carrier comprising a target nucleotide sequence, and the like. The nucleocapsid assembling necessary element disclosed by the invention comprises a 45-65 map unit positioned in a viral genome of a hepatitis A baculovirus, preferably, a DNA sequence in the position of a 47-61 map unit. An NAE sequence plays an essential role in the nucleocapsid assembling process. The baculovirus has wide application prospect in the gene transduction field, so that the NAE sequence found in the invention can improve a baculovirus gene-transduced carrier, so that the establishment process is simpler and more convenient, the target DNA is higher in capacity, and higher biosecurity is achieved; and therefore, the nucleocapsid assembling necessary element is applicable to a required gene transduction operation, and requirements of clinical application, such as gene therapy and the like, can be better satisfied.

Provided are a phthalazinone compound, method for preparation thereof, pharmaceutical composition thereof, and a use thereof; the structure of said phthalazinone compound is as represented by formula I; the compound of said formula I is targeted to a viral nucleocapsid; it can inhibit the replication of a virus by means of interference of the viral nucleocapsid, has a potent activity for inhibiting HBV DNA replication and a good liver targeting, can stably exist and enrich in the liver, and is a new and effective anti-HBV inhibitor.