37 results about "Viral nucleocapsid" patented technology

The invention relates to a method for marking enveloped virus nucleocapsid by using a quantum dot. The method is characterized in that a capsid protein sequence blended with a biotin receptor peptide sequence and a catalytic receptor peptide biotinylation enzyme sequence are simultaneously inserted in a virus whole-genome so as to obtain a recombinant virus plasmid, the biotinylation of the nucleocapsid is realized by virtue of the reproduction process of the virus plasmid in a host cell, and then the quantum dot coupled with streptavidin is used for realizing the marking of the enveloped virus nucleocapsid. The method does not needs the violent chemical reaction transformation and the marking of a virus and is beneficial to the maintenance of infection activity of the virus self; and the marking of the nucleocapsid in the enveloped virus does not affect the surface of the enveloped virus and the indentation of the surface. The method can be applied to the virus infection mechanism study and the virus-related disease treatment research.

The invention relates to detection kits in the technical field of biology, in particular to a polypeptide-ELISA (enzyme-linked immunosorbent assay) kit for detecting a specific antibody of an N (nucleocapsid) protein of an SFTSV (severe fever with thrombocytopenia syndrome virus). The kit comprises an ELISA plate pre-coated with synthetic SFTSV N protein linear B-cell antigenic polypeptide, a sample diluent, negative control serum, positive control serum, an HRP (horse radish peroxidase) marked antibody, a concentrated cleaning solution, an enzyme substrate solution and a stop buffer. The kit has the advantages of high specificity, good repeatability and simplicity, convenience and rapidness in operation, and can be widely used for evaluating SFTSV infection conditions.

The invention is a melanodermia virus quickly-detecting reagent box, including: a detecting device; tween-phosphate buffer liquid containing ox serum albumin; tween-phosphate buffer liquid; low osmotic liquid; gold labeled anti-melanodermia virus monoclonal antibody probe, prepared of two colloidal gold labeled monoantibodies E and D in proportion; the E and D are anti-melanodermia virus bursa membrane antibody E and anti-melanodermia virus nucleus coat antibody D secreted by two hybrid tumor cells, respectively; the conservation numbers of the two hybrid tumor cells are CCTCC-C200421 and CCTCC-C200422, respectively. As the E and D are used as detecting antibodies, they can obviously improve detecting sensitivity. The detecting reaction only takes 3 minutes without hatching and any instrument and equipment, and their detecting sensitivity is the same as that of spot immune printing method. The reagent box has the characters: simple and convenient, quick, accurate, etc.

The invention relates to the field of biological medicine, in particular to an SARS-CoV-2 detection kit based on an indirect method. The kit comprises (a) a solid phase conjugate. The solid-phase conjugate consists of a solid-phase carrier as well as an antigen and an antibody which coat the solid-phase carrier. The antibody is a monoclonal antibody for resisting novel coronavirus nucleocapsid N protein. The antigen comprises an S1 subunit, an S2 subunit, an RBD, an N protein and an E protein of the SAR-CoV-2 Spike protein. The kit further comprises (b) an anti-antibody and an antibody marked with a chemiluminescent marker. The antibody is a monoclonal antibody to the novel coronavirus nucleocapsid N protein, but is bound to a different epitope from the antibody in (a). The anti-antibody is at least one of an anti-human IgG secondary antibody, an anti-human IgA secondary antibody and an anti-human IgM secondary antibody. The anti-antibody is a monoclonal antibody or a polyclonal antibody.

The invention discloses a double-target SARS-CoV-2 virus nucleic acid detection primer group, application and a fluorescent kit, and relates to the technical field of virus nucleic acid detection methods. The double-target specific nucleic acid detection primer group is designed for a conserved region of the SARS-CoV-2 virus by selecting double gene sequences of the SARS-CoV-2 virus nucleocapsid protein N gene and the spike protein S gene, and the SARS-CoV-2 virus can be specifically detected. The invention further provides a one-pot RT-LAMP fluorescence detection kit for detecting the SARS-CoV-2 virus, a throat swab and other samples, nucleic acid extraction is not needed, an amplification curve is observed through a fluorescence amplification instrument under the isothermal condition of 65 DEG C, the SARS-CoV-2 virus can be accurately detected within 30 minutes, and virus-result detection is directly achieved. The fluorescent kit adopts a double-primer group design, is strong in specificity, high in sensitivity, free of cross reaction, capable of rapidly and accurately screening the SARS-CoV-2 virus, simple to operate and high in safety; the detection result is visual, the judgment operation is simple, and the method has good application advantages in the field of viral nucleic acid detection.

The invention discloses a standard positive reference material for rana grylio virus (RGV) detection. In the invention, a pair of primers are firstly designed, which can specifically amplify capsid protein complete genes of soft-shelled turtle iridovirus nuclears, wherein the nucleotide sequences of the primers are shown in SEQ ID.1 and SEQ ID NO.2; the capsid protein complete genes of the soft-shelled turtle iridovirus nuclears amplified by the primers have the nucleotide sequence shown in SEQ ID NO.3; and a recombinant plasmid pGEM-T-S containing the capsid protein complete genes of the soft-shelled turtle iridovirus nuclears is constructed and screened after a proper PCR reaction system and reaction conditions are found out, which can be used as the standard positive reference material for molecular biology detection on STIV MCP, EHNV MCP and TFV MCP.

The invention provides a method for preparing a polyclonal antibody to rice stripe virus nucleocapsid protein and its application, and belongs to the technical field of rice stripe virus detection. The polyclonal antibody to rice stripe virus nucleocapsid protein CP is based on rice stripe virus The nucleocapsid protein CP is obtained by immunizing animals with an immunogen; the amino acid sequence of the rice stripe virus nucleocapsid protein CP is shown in SEQ ID No.1. The polyclonal antibody of the rice stripe virus nucleocapsid protein of the present invention has the characteristics of low cost, wide application, multiple audiences, high sensitivity, etc., and can be used in immunospot assay, enzyme-linked immunosorbent assay, western blot, immuno A number of biological detection experiments such as fluorescence method and immunoelectron microscope method. Using the polyclonal antibody of the present invention, the early warning of the disease is carried out by the striatellus striatellus at the beginning of the outbreak of the rice stripe leaf blight, which is beneficial to the multi-means disease monitoring.

According to the application of the neferine in preparation of the anti-hepatitis B virus medicine, the neferine targets hepatitis B virus RNA and interferes with synthesis of the hepatitis B virus RNA, so that transcription of pgRNA, preS / S RNA and X RNA of the virus is reduced, reverse transcription synthesis of cDNA in a virus nucleocapsid in a cell and synthesis of virus protein are reduced, and therefore, the anti-hepatitis B virus medicine is obtained. Therefore, the synthesis and secretion of antigens HBsAg and HBeAg are reduced. According to the application disclosed by the invention, an in-vitro cell model is applied, the neferine is found to have an inhibiting effect on the HBV for the first time, and a new clue is provided for subsequent in-depth research and clinical medication of resisting the HBV.

A method for preparing a precious metal palladium nanorod by means of cotton bollworm baculovirus nucleocapsid mainly includes the steps that cotton bollworm karyotype polyhedron virus raw powder is dispersed through ultra-pure water to obtain grey polyhedrin suspension, alkaline hydrolysis liquid is added into the grey polyhedrin suspension, the mixture stands still for 15-25 min, then the pH value is adjusted to be 7 through acetic acid, chloroform is added, centrifugalization is carried out for 5-10 min at the speed of 5000-6000 r / min, supernatant fluid is sucked, centrifugalization is carried out for 1-2 h at the temperature of 4-10 DEG C and at the speed of 25000-30000 r / min, pigmentation is re-suspeneded through a PBS solution, and a cotton bollworm baculovirus nucleocapsid solution is obtained; a PdCl2 solution is added into the cotton bollworm baculovirus nucleocapsid solution and fully and evenly mixed, incubation is carried out in a shaking mode for 15-25 h at the temperature of 20-30 DEG C and at the speed of 130-170 r / min, and the precious metal palladium nanorod loaded by the cotton bollworm baculovirus nucleocapsid is obtained. The method is simple in process, environmentally friendly and efficient, industrial production is easy to achieve, and the prepared palladium nanorod is stable in appearance and uniform in size.

The invention relates to the assembling field of virus nucleocapsid, particularly to a baculovirus nucleocapsid assembling necessary element and an application thereof in nucleocapsid assembling, establishment of a carrier comprising a target nucleotide sequence, and the like. The nucleocapsid assembling necessary element disclosed by the invention comprises a 45-65 map unit positioned in a viral genome of a hepatitis A baculovirus, preferably, a DNA sequence in the position of a 47-61 map unit. An NAE sequence plays an essential role in the nucleocapsid assembling process. The baculovirus has wide application prospect in the gene transduction field, so that the NAE sequence found in the invention can improve a baculovirus gene-transduced carrier, so that the establishment process is simpler and more convenient, the target DNA is higher in capacity, and higher biosecurity is achieved; and therefore, the nucleocapsid assembling necessary element is applicable to a required gene transduction operation, and requirements of clinical application, such as gene therapy and the like, can be better satisfied.

Provided are a phthalazinone compound, method for preparation thereof, pharmaceutical composition thereof, and a use thereof; the structure of said phthalazinone compound is as represented by formula I; the compound of said formula I is targeted to a viral nucleocapsid; it can inhibit the replication of a virus by means of interference of the viral nucleocapsid, has a potent activity for inhibiting HBV DNA replication and a good liver targeting, can stably exist and enrich in the liver, and is a new and effective anti-HBV inhibitor.

Provided are a phthalazinone compound, method for preparation thereof, pharmaceutical composition thereof, and a use thereof; the structure of said phthalazinone compound is as represented by formula I; the compound of said formula I is targeted to a viral nucleocapsid; it can inhibit the replication of a virus by means of interference of the viral nucleocapsid, has a potent activity for inhibiting HBV DNA replication and a good liver targeting, can stably exist and enrich in the liver, and is a new and effective anti-HBV inhibitor.

The present invention provides a BHK-21 cell line stably expressing Schmallenberg virus (SBV) nucleocapsid protein, its preservation number is CGMCC No.7616, named BHK-21-pLV-EGFP-SBV-N . The results of cell immunofluorescence test showed that the cell line not only reacted specifically with SBV monoclonal antibody 2C8, but also recognized the positive serum of SBV-infected cattle. The BHK‑21 cell line stably expressing SBV N protein can be used for the detection of Schmallenberg disease and has a good application prospect.

The invention discloses the application of β-defensin-1 in the preparation of medicines for treating or preventing hepatitis B virus infection. Study on the effect of β-defensin-1 on hepatitis B virus by co-transfection of β-defensin-1 overexpression plasmid or small interfering RNA targeting β-defensin-1 and HBV1.3 ploidy plasmid into liver cells Effects on replication, the surface antigen and e-antigen secreted by the virus were examined, as well as the levels of intracellular viral nucleocapsid-associated DNA, showing that β‑defensin‑1 inhibits HBV replication in hepatocytes. β-defensin-1 may affect the activity of HBV or affect the replication process of HBV infection or after infection. Therefore, by using alone or in combination with other anti-HBV drugs, β-defensin-1 can play an important role in the prevention or treatment of hepatitis B virus infection. Human β‑defensin‑1 is an antimicrobial polypeptide constitutively expressed in the human body, and has no toxic side effects on the human body at physiological concentrations.