SARS-CoV-2 detection kit

A detection kit and kit technology, applied in the field of biomedicine, can solve problems such as unsatisfactory antibody sensitivity and limited sampling methods, and achieve the effects of saving operating time and cost, shortening the diagnostic window period, and preventing missed detection

Active Publication Date: 2021-06-15
SHENZHEN YHLO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current method of confirming the diagnosis of COVID-19 is to use real-time fluorescent RT-PCR to detect the positive nucleic acid of the new coronavirus, or to sequence the virus gene. Due to the limitations of the sampling method
[0008] However, at present, the sensitivity of antibody detection is still not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] This kit is mainly divided into calibrator and detection reagent, among which, the detection reagent consists of 3 parts:

[0097] 1.1R1 magnetic bead components:

[0098] Composed of 7 kinds of paramagnetic magnetic beads and magnetic bead diluent, the combination of magnetic bead coating protein is as follows:

[0099] ① Anti-new coronavirus N protein mouse monoclonal antibody;

[0100] ② SARS-CoV-2 N protein;

[0101] ③SARS-CoV-2 Spike protein S1 subunit;

[0102] ④SARS-CoV-2 Spike protein S2 subunit;

[0103] ⑤ SARS-CoV-2 Spike protein S1 subunit receptor binding domain (Receptor Binding Domian, RBD) protein;

[0104] ⑥SARS-CoV-2 M protein;

[0105] ⑦ SARS-CoV-2 E protein;

[0106] Among them, the anti-viral N protein mouse monoclonal antibody can be extracted from mouse ascites or hybridoma cells in vitro, and can be a variety of monoclonal antibodies against different immune sites of different N proteins; S1, S2, RBD, M, and E proteins can be extracted natu...

Embodiment 2

[0122] This kit is mainly divided into calibrator and detection reagent, among which, the detection reagent consists of 3 parts:

[0123] 1.1R1 magnetic bead components:

[0124] Composed of 7 kinds of paramagnetic magnetic beads and magnetic bead diluent, the combination of magnetic bead coating protein is as follows:

[0125] ① Anti-new coronavirus N protein mouse monoclonal antibody;

[0126] ② SARS-CoV-2 N protein;

[0127] ③SARS-CoV-2 Spike protein S1 subunit;

[0128] ④SARS-CoV-2 Spike protein S2 subunit;

[0129] ⑤ SARS-CoV-2 Spike protein S1 subunit receptor binding domain (Receptor Binding Domian, RBD) protein;

[0130] ⑥SARS-CoV-2 M protein;

[0131] ⑦ SARS-CoV-2 E protein;

[0132] Among them, the anti-viral N protein mouse monoclonal antibody can be extracted from mouse ascites or hybridoma cells in vitro, and can be a variety of monoclonal antibodies against different immune sites of different N proteins; S1, S2, RBD, M, and E proteins can be extracted natu...

Embodiment 3

[0148] This kit is mainly divided into calibrator and detection reagent, among which, the detection reagent consists of 3 parts:

[0149] 1.1R1 magnetic bead components:

[0150] Composed of 5 kinds of paramagnetic magnetic beads and magnetic bead diluent, the combination of magnetic bead coating protein is as follows:

[0151] ① Anti-new coronavirus N protein mouse monoclonal antibody, which can form a paired antibody with the monoclonal antibody in the R1 magnetic bead component to detect the N protein antigen.

[0152] ② SARS-CoV-2 N protein;

[0153] ③SARS-CoV-2 Spike protein S1 subunit;

[0154] ④SARS-CoV-2 Spike protein S2 subunit;

[0155] ⑤ SARS-CoV-2 Spike protein S1 subunit receptor binding domain (Receptor Binding Domian, RBD) protein;

[0156] ① and ② have no specific binding.

[0157] Among them, the anti-viral N protein mouse monoclonal antibody can be extracted from mouse ascites or hybridoma cells in vitro, and can be a variety of monoclonal antibodies aga...

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Abstract

The invention relates to an SARS-CoV-2 detection kit. The kit comprises: (a) a solid phase conjugate. The solid-phase conjugate consists of a solid-phase carrier as well as an antigen and an antibody which coat the solid-phase carrier. The antibody is a monoclonal antibody for resisting novel coronavirus nucleocapsid N protein. The antigen comprises an N protein of recombinant SARS-CoV-2. The kit further comprises (b) an antigen marked with a chemiluminescent marker, and an antibody and / or an anti-antibody marked with a chemiluminescent marker. The antibody is a monoclonal antibody to the novel coronavirus nucleocapsid N protein, but is bound to a different epitope from the antibody in (a). The antigen comprises an N protein of recombinant SARS-CoV-2. The anti-antibody is at least one of an anti-human IgG secondary antibody, an anti-human IgA secondary antibody and an anti-human IgM secondary antibody. The anti-antibody is a monoclonal antibody or a polyclonal antibody. In (a) and (b), the N protein of the recombinant SARS-CoV-2 does not have the antigen epitope recognized by the monoclonal antibody for resisting the novel coronavirus nucleocapsid N protein.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a SARS-CoV-2 detection kit. Background technique [0002] The new type of coronavirus (SARS-CoV-2) belongs to the genus β coronavirus, the particles are round or oval, with a diameter of 60nm-140nm, and compared with SARS, it has a higher infectivity. The new coronavirus has a strong ability to spread from person to person, and the crowd is generally susceptible. Patients are the most important source of infection. Therefore, early detection, early isolation, and early treatment of asymptomatic infections, suspected and confirmed cases are still very necessary . How to accurately detect the virus, quickly identify patients with new crown infection, and reduce cross-infection; and how to effectively monitor the disease process and antibody production will be the top priority. Based on epidemiological surveys, the incubation period of the disease COVID-19 caused by the novel coronaviru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/68G01N33/58G01N33/543
CPCG01N33/56983G01N33/6854G01N33/58G01N33/543G01N2333/165
Inventor 钱纯亘汪大明夏福臻王刚祝亮林晓涛胡鹍辉
Owner SHENZHEN YHLO BIOTECH
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