Method for preparing precious metal palladium nanorod by means of cotton bollworm baculovirus nucleocapsid
A technology of baculovirus and cotton bollworm, which is applied in the field of preparation of nanomaterials, can solve the problems of unsatisfactory morphology and dimensional stability, increase the complexity of experimental process, and different morphology of palladium nanorods, and achieve uniform size. , The effect of low production cost and stable appearance
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Embodiment 1
[0019] Disperse 50 mg of cotton bollworm nuclear polyhedrosis virus powder with 5 mL of cold ultrapure water. Prepared by water meter, obtain off-white polyhedron suspension, add alkaline hydrolysis solution 1.25mL to the polyhedron suspension and let stand for 15min, the alkaline hydrolysis solution is 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and a mixed solution of 0.03mol / L disodium edetate. Adjust the pH to 7 with 0.5 mol / L glacial acetic acid, add 1.25 mL of chloroform, and shake for 5 min. After centrifuging at 5000r / min for 10min, suck out the supernatant, the upper layer liquid, put each 4mL supernatant into an ultracentrifuge tube, centrifuge at 28000r / min at 4°C for 1h, and wash the precipitated spots with 1.33mL PBS solution Resuspend to obtain the baculovirus nucleocapsid solution of cotton bollworm.
[0020] Such as figure 1 As shown, it can be seen that the template used is a rod-shaped structure. The nucleocapsid of the cotton bollworm baculovirus ...
Embodiment 2
[0024] Disperse 50 mg of cotton bollworm nuclear polyhedrosis virus powder with 5 mL of cold ultrapure water. Prepared by water meter, obtain off-white polyhedron suspension; Add alkaline hydrolyzate 0.83mL to polyhedron suspension and leave standstill 25min, described alkaline hydrolyze is 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and A mixed solution of 0.03mol / L disodium edetate. Adjust the pH to 7 with 0.5 mol / L glacial acetic acid, add 1.46 mL of chloroform, and shake for 8 min. After centrifuging at 6000r / min for 5min, suck out the supernatant, that is, the upper layer liquid, put each 6mL supernatant into an ultracentrifuge tube, centrifuge at 10°C and 30000r / min for 2h, and use 1.2mL of each precipitated spot The PBS solution is resuspended to obtain the nucleocapsid solution of the cotton bollworm baculovirus.
[0025] Such as figure 2 As shown, it can be seen that the baculovirus nucleocapsid is composed of multiple helical capsid particles. The essence...
Embodiment 3
[0029] Disperse 50 mg of cotton bollworm nuclear polyhedrosis virus powder with 5 mL of cold ultrapure water. Prepared by a water meter, obtain off-white polyhedron suspension, add 1mL alkaline hydrolysis solution to the polyhedron suspension and let stand for 20min, the alkaline hydrolysis solution is 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and A mixed solution of 0.03mol / L disodium edetate. Adjust the pH to 7 with 0.5 mol / L glacial acetic acid, add 1 mL of chloroform, and shake for 10 min. After centrifuging at 5500r / min for 10min, suck out the upper layer liquid, i.e. the supernatant, put each 5mL supernatant into an ultracentrifuge tube, centrifuge at 25000r / min at 6°C for 1.5h, and use 1.25 Resuspend in mL PBS solution to obtain the nucleocapsid solution of cotton bollworm baculovirus.
[0030] Take 300 μL of the baculovirus nucleocapsid solution of cotton bollworm and add 75 μL of PdCl with a concentration of 10 mM 2 The solution was thoroughly mixed and i...
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