Method for preparing precious metal palladium nanorod by means of cotton bollworm baculovirus nucleocapsid

A technology of baculovirus and cotton bollworm, which is applied in the field of preparation of nanomaterials, can solve the problems of unsatisfactory morphology and dimensional stability, increase the complexity of experimental process, and different morphology of palladium nanorods, and achieve uniform size. , The effect of low production cost and stable appearance

Active Publication Date: 2014-08-27
YANSHAN UNIV
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Problems solved by technology

Among them, the use of biological templates to prepare palladium nanomaterials has become a new trend. For example, Alexander S. Freer et al. (Journal of Colloid and Interface Science, 2013, 392, 213–218) prepared palladium nanorods using tobacco mosaic virus biological template synthesis. , they first genetically modified the tobacco mosaic virus to change the amino acids of the capsid protein in order to better adsorb palladium nanoparticles, but at the same time it also increased the complexity of the experimental process
The preparation process is complicated and the preparation period is prolonged
Elizabeth S. Royston et al. (Journal of Colloid and Interface Science, 2009, 332, 402–407) also used tobacco mosaic virus as the initial template, attached silicon to it, and then prepared palladium nanorods with the silicon / virus composite structure as a template. The process is complicated, which increases the production cost
Yi Hsiu Chen et al. (J.Am.Chem.Soc,2009,131,9114–9121.) used a seed-mediated method to synthesize palladium nanorods. Although the synthesis process was simplified to a certain extent, the synthesized palladium The morphology of the nanorod junction is different, and the structure is not stable enough
There are also some scholars who prepare palladium nanorods by electrochemical methods, but the shape and size stability are not ideal.

Method used

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  • Method for preparing precious metal palladium nanorod by means of cotton bollworm baculovirus nucleocapsid
  • Method for preparing precious metal palladium nanorod by means of cotton bollworm baculovirus nucleocapsid
  • Method for preparing precious metal palladium nanorod by means of cotton bollworm baculovirus nucleocapsid

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Experimental program
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Effect test

Embodiment 1

[0019] Disperse 50 mg of cotton bollworm nuclear polyhedrosis virus powder with 5 mL of cold ultrapure water. Prepared by water meter, obtain off-white polyhedron suspension, add alkaline hydrolysis solution 1.25mL to the polyhedron suspension and let stand for 15min, the alkaline hydrolysis solution is 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and a mixed solution of 0.03mol / L disodium edetate. Adjust the pH to 7 with 0.5 mol / L glacial acetic acid, add 1.25 mL of chloroform, and shake for 5 min. After centrifuging at 5000r / min for 10min, suck out the supernatant, the upper layer liquid, put each 4mL supernatant into an ultracentrifuge tube, centrifuge at 28000r / min at 4°C for 1h, and wash the precipitated spots with 1.33mL PBS solution Resuspend to obtain the baculovirus nucleocapsid solution of cotton bollworm.

[0020] Such as figure 1 As shown, it can be seen that the template used is a rod-shaped structure. The nucleocapsid of the cotton bollworm baculovirus ...

Embodiment 2

[0024] Disperse 50 mg of cotton bollworm nuclear polyhedrosis virus powder with 5 mL of cold ultrapure water. Prepared by water meter, obtain off-white polyhedron suspension; Add alkaline hydrolyzate 0.83mL to polyhedron suspension and leave standstill 25min, described alkaline hydrolyze is 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and A mixed solution of 0.03mol / L disodium edetate. Adjust the pH to 7 with 0.5 mol / L glacial acetic acid, add 1.46 mL of chloroform, and shake for 8 min. After centrifuging at 6000r / min for 5min, suck out the supernatant, that is, the upper layer liquid, put each 6mL supernatant into an ultracentrifuge tube, centrifuge at 10°C and 30000r / min for 2h, and use 1.2mL of each precipitated spot The PBS solution is resuspended to obtain the nucleocapsid solution of the cotton bollworm baculovirus.

[0025] Such as figure 2 As shown, it can be seen that the baculovirus nucleocapsid is composed of multiple helical capsid particles. The essence...

Embodiment 3

[0029] Disperse 50 mg of cotton bollworm nuclear polyhedrosis virus powder with 5 mL of cold ultrapure water. Prepared by a water meter, obtain off-white polyhedron suspension, add 1mL alkaline hydrolysis solution to the polyhedron suspension and let stand for 20min, the alkaline hydrolysis solution is 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and A mixed solution of 0.03mol / L disodium edetate. Adjust the pH to 7 with 0.5 mol / L glacial acetic acid, add 1 mL of chloroform, and shake for 10 min. After centrifuging at 5500r / min for 10min, suck out the upper layer liquid, i.e. the supernatant, put each 5mL supernatant into an ultracentrifuge tube, centrifuge at 25000r / min at 6°C for 1.5h, and use 1.25 Resuspend in mL PBS solution to obtain the nucleocapsid solution of cotton bollworm baculovirus.

[0030] Take 300 μL of the baculovirus nucleocapsid solution of cotton bollworm and add 75 μL of PdCl with a concentration of 10 mM 2 The solution was thoroughly mixed and i...

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Abstract

A method for preparing a precious metal palladium nanorod by means of cotton bollworm baculovirus nucleocapsid mainly includes the steps that cotton bollworm karyotype polyhedron virus raw powder is dispersed through ultra-pure water to obtain grey polyhedrin suspension, alkaline hydrolysis liquid is added into the grey polyhedrin suspension, the mixture stands still for 15-25 min, then the pH value is adjusted to be 7 through acetic acid, chloroform is added, centrifugalization is carried out for 5-10 min at the speed of 5000-6000 r/min, supernatant fluid is sucked, centrifugalization is carried out for 1-2 h at the temperature of 4-10 DEG C and at the speed of 25000-30000 r/min, pigmentation is re-suspeneded through a PBS solution, and a cotton bollworm baculovirus nucleocapsid solution is obtained; a PdCl2 solution is added into the cotton bollworm baculovirus nucleocapsid solution and fully and evenly mixed, incubation is carried out in a shaking mode for 15-25 h at the temperature of 20-30 DEG C and at the speed of 130-170 r/min, and the precious metal palladium nanorod loaded by the cotton bollworm baculovirus nucleocapsid is obtained. The method is simple in process, environmentally friendly and efficient, industrial production is easy to achieve, and the prepared palladium nanorod is stable in appearance and uniform in size.

Description

technical field [0001] The invention relates to a preparation method of nanomaterials, in particular to a preparation method of precious metal palladium nanorods. Background technique [0002] Due to the surface-interface effect, small size effect, quantum size effect, and macroscopic quantum tunneling of noble metal nanomaterials, noble metal nanomaterials exhibit unique mechanical, electrical, optical, thermal, magnetic, catalytic, and superconducting properties. These unique properties make it have important application value in the fields of optics, sensors, catalysis and biological detection and analysis, so it has attracted more and more attention from people. Another major feature of nanomaterials is that their properties will change with changes in size, shape, and composite structure. Therefore, people expect that the structure, shape, and size can be adjusted, uniformly distributed, and stable through a simple and green method. excellent noble metal nanomaterials....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24
Inventor 高发明罗京京庞桂桂王纪平刘鹏
Owner YANSHAN UNIV
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