65 results about "Taxus cuspidata" patented technology

The invention relates to an extraction method of taxol from branches and leaves of artificially cultivated yew. A plurality of methods, such as planting of stocky yew, cultivating of tissue and semi-synthesis and total synthesis of taxol, have been developed home and abroad so as to obtain taxol raw material; however, the tissue cultivating method has low yield and high broth price; and the totalsynthesis has extremely low yield, worse therapeutic effect than natural-source taxol, and a high cost; thereby being difficult for commercial application. The invention uses branches and leaves of artificially cultivated taxus media, Yunnan taxus, southern taxus or northeastern taxus as raw materials; the raw materials are marinated by carbinol and extracted by petroleum oil and propyl acetate insequence; and normal-pressure normal-phase silica gel column chromatography or reverse-phase high-pressure liquid chromatography is used to obtain high-purity taxol with a purity being higher than 99.6%. The preparation method has simple process, low cost, recyclable solvent and eluant; therefore, the method is applicable to industrial production and protects plant resource.

The invention relates to a cuttage breeding method of taxus chinensis in northeast, comprising the following steps: selecting a one-year-old branch as a branch for cuttage; concealing the branch at a shade place, and carrying the branch to a destination after the branch is packaged by a plastic bag; retaining 1-2 leaves on the upper end of each nursery stock stalk when shearing a seedling, shearing the parts longer than 3cm of the retained leaves, and also shearing leaves inserted into the stalk positioned in the middle of a seedling bed; disinfecting a land before the cuttage so as to reducerhizoctonia solani kuhn, and carrying out the cuttage from the middle ten days of October to the first ten days of November or from February to March; using a light shielding net of 80 percent to shield light without directly shining with the sun during the daily management of the seedling; controlling the soil water content at 35-45 percent; and paying attention to the water drainage of the seedling in rainy days and intertillage weed control in ordinary days, and preventing and curing diseases of the seedling in time by chemical agents. By adopting the cuttage breeding method, the taxus chinensis in northeast has high survival rate, fast breeding cultivation and high disease resistance.