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Method for extracting and purifying 7-xylose-10-deacetyl paclitaxel from taxus cuspidata

A technology of acetyl paclitaxel and yew, applied in chemical instruments and methods, separation methods, sugar derivatives, etc., can solve the loss of 7-xylose-10-deacetyl paclitaxel, long purification process, backward extraction process, etc. problem, to achieve the effect of simple and easy method, short cycle, efficient and reasonable utilization

Inactive Publication Date: 2009-11-11
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, in the process of separating and purifying 7-xylose-10-deacetyl-paclitaxel using plant resources, the main influencing factors are the backward extraction process and the ubiquity of 7-xylose-10- The loss of deacetyl paclitaxel and the long purification process increase the cost

Method used

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  • Method for extracting and purifying 7-xylose-10-deacetyl paclitaxel from taxus cuspidata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Weigh 1000g of fresh twigs and leaves of Taxus chinensis (dry weight is 200g), add 2000mL of 50-60℃ warm water to extract twice, and then extract continuously twice, each time for 30min.

[0018] At the same time, 400g of macroporous adsorption resin AB-8 is packed into a column by wet method, and the liquid level is retained, and 4000ml of the extracted medicinal solution is passed through the adsorption column at a flow rate of 2400-3200mL per hour. Impurities were washed with 2BV of 30% ethanol, and then eluted with 6BV of 80% ethanol. The effluent was collected and concentrated to obtain a concentrate.

[0019] 200 grams of 300-400 mesh silica gel was applied to the column with chloroform wet method, and the eluate was concentrated to dry powder and mixed with silica gel in equal proportions, and purified on the silica gel column. When the volume ratio of the eluent (chloroform:methanol) was 12:1, 200 mg of crude 7-xylose-10-desacetyl-paclitaxel was eluted.

[0020...

Embodiment 2

[0022] Weigh 2kg of dried branches and leaves of Taxus chinensis, add 18L of warm water at 50-60°C to extract twice, and then extract twice continuously, each time for 30min.

[0023] At the same time, 4 kg of macroporous adsorption resin AB-8 was packed into a column by a wet method, and the liquid level was retained, and 36 L of the extracted medicinal solution was passed through the adsorption column at a flow rate of 24 to 32 L per hour. Impurities were washed with 2BV of 30% ethanol, and then eluted with 6BV of 80% ethanol. The effluent was collected and concentrated to obtain a concentrate.

[0024] 2kg of 300-400 mesh silica gel was applied to the column with chloroform wet method, and the eluate was concentrated to dry powder and mixed with silica gel in equal proportions, and purified on the silica gel column. When the volume ratio of the eluent (chloroform:methanol) was 12:1, 2 g of crude 7-xylose-10-desacetyl-paclitaxel was eluted.

[0025] The mobile phase in whic...

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Abstract

The invention relates to a method for extracting and purifying a functional element from a traditional Chinese herb and aims at providing a convenient, safe, economical and efficient method for extracting and purifying 7-xylose-10-deacetyl paclitaxel which is a monomeric compound from taxus cuspidata. The technical proposal adopted by the invention comprises the steps: fresh or dried leaves of taxus cuspidate are taken as raw materials, and macroporous adsorption resin enrichment technology, normal-pressure chromatography preparation technology and high-pressure liquid phase separation technology are combined to prepare the 7-xylose-10-deacetyl paclitaxel with the purity higher than 99.90 percent at an extraction rate of 0.05 percent. The raw materials used in the invention can be waste materials from the paclitaxel production, thus fully utilizing natural resources. Moreover, the method comprises extraction, primary impurity removal and repurification, thus being simple and easy; and the loss of the target compound is low.

Description

technical field [0001] The invention relates to a method for separating and purifying functional components from plants, in particular to extracting and purifying 7-xylose-10-deacetyl paclitaxel from fresh or dry branches and leaves of Taxus chinensis. Background technique [0002] Paclitaxel is widely used in the treatment of ovarian cancer, breast cancer, lung cancer and other tumors. However, because it is insoluble in water, it needs to be dissolved in a mixed solvent of polyoxyethylene castor oil (cremophor EL) and absolute ethanol to increase water solubility, and polyoxyethylene castor oil (cremophor EL) releases histamine when degraded in the body, resulting in Different degrees of allergic reactions can also cause granule release and demyelination changes in nerve cells and aggravate the peripheral neurotoxicity of paclitaxel. In order to reduce toxicity and improve its curative effect, synthetic and semi-synthetic paclitaxel compounds are one of the methods to sol...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08B01D11/02B01D15/00
Inventor 祖元刚姜守刚付玉杰李庆勇张宝友张宇汪琢孙蕊史伟国
Owner NORTHEAST FORESTRY UNIVERSITY
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