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Tissue culture method of taxus cuspidata

A tissue culture and taxus chinensis technology, applied in the field of plant cultivation, can solve the problems of low germination rate and survival rate, affecting the industrialized production of Taxus chinensis, slow growth cycle, etc., and achieves short tissue culture cycle, strong proliferation ability and survival rate. high effect

Inactive Publication Date: 2016-11-23
夏学云
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of pre-breeding planting method and artificial cutting method will be limited by seedling resources, coupled with low germination rate and survival rate, and slow growth cycle, which seriously affects the industrial production of yew

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Select the middle layer cells of the new branches of Taxus chinensis, process them into 2cm tissue slices, wipe them with 3% potassium permanganate first, and then wipe them with 90% ethanol solution for disinfection; add 0.3 mg / L 1-naphthyl acetic acid and 1.5% hydrolyzed complex, and keep the temperature at 20°C and the indoor humidity at 62%, culture the sterilized tissue slices in the above B5 medium for 15 days to form 12cm tissue, Select the middle 5cm for the next step of cultivation;

[0016] (2) Add 2mg / L of 1-naphthaleneacetic acid, 2mg / L of indoleacetic acid, and 0.5mg / L of 2,4-dichlorophenoxyacetic acid to the rooting medium; transplant the above callus to the rooting culture Carry out rooting culture in the base, and keep the dark environment for the first 3 days, and keep the light time 14h / d after 3 days, until the main root of 3cm grows.

Embodiment 2

[0018] (1) Select the middle layer cells of the new branches of Taxus chinensis, process them into 1cm tissue slices, first wipe them with 3% potassium permanganate, and then wipe them with 90% ethanol solution for disinfection; add 0.5 mg / L 1-Naphthylacetic acid and 1.3% hydrolyzed complex, and keep the temperature at 22°C and the indoor humidity at 60%, culture the sterilized tissue slices in the above B5 medium for 20 days to form 10cm tissue, Select the middle 6cm for the next step of cultivation;

[0019] (2) Add 3mg / L of 1-naphthylacetic acid, 1mg / L of indoleacetic acid, and 1.0mg / L of 2,4-dichlorophenoxyacetic acid to the rooting medium; transplant the above calli into rooting culture The rooting culture was carried out in the medium, and the environment was kept in the dark for the first 3 days, and after 3 days, the light time was kept 12h / d until the main root of 30mm grew.

Embodiment 3

[0021] (1) Select the middle layer cells of the new branches of Taxus chinensis, process them into 2cm tissue slices, wipe them with 3% potassium permanganate first, and then wipe them with 90% ethanol solution for disinfection; add 0.4 mg / L 1-Naphthylacetic acid and 1.4% hydrolyzed complex, and keep the temperature at 21°C, and the indoor humidity at 61%, culture the sterilized tissue slices in the above-mentioned B5 medium for 17 days to form 11cm tissue, Select the middle 5-6cm for the next step of cultivation;

[0022] (2) Add 2.5mg / L of 1-naphthaleneacetic acid, 1.5mg / L of indoleacetic acid, and 0.7mg / L of 2,4-dichlorophenoxyacetic acid to the rooting medium; transplant the above-mentioned calli into Carry out the rooting culture in the rooting medium, and keep the dark environment for the first 3 days, and keep the light time 13h / d after 3 days, until the main root of 3cm grows.

[0023] The beneficial effects of the present invention are as follows: (1) the middle laye...

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Abstract

The invention discloses a tissue culture method of taxus cuspidata. The method includes the following steps that 1, intermediate cells of a newborn branch of taxus cuspidata are selected and treated into a 2 cm tissue slice, the tissue slice is disinfected and then cultured for 15-20 days in a B5 culture medium to form 10-12 cm tissue, and the 5-6 cm part in the middle is selected for the next step of culture; 2, the callus is transplanted into a rooting culture medium for rooting culture, a dark environment is kept in the first three days, and the illumination time of 12-14 h / d is kept three days later till 3 cm main roots emerge. According to the tissue culture method of taxus cuspidata, the intermediate cells of the newborn branch of taxus cuspidata are selected, contain rich taxol and have high proliferation capability, so that the tissue culture period of taxus cuspidata is short, and the survival rate is high; the selected culture medium is simple, easy to obtain and suitable for large-scale culture of taxus cuspidata seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue cultivation of yew. Background technique [0002] Taxol extracted from the yew plant is a new type of anti-cancer drug developed in recent years, which has a good therapeutic effect on cancer. At the same time, the yew is an endangered and rare species under national first-class protection, especially the wild yew has been degraded. Deforestation is exhausting. Due to the huge demand for anticancer drugs in the market, the artificial cultivation of yew is particularly important. [0003] At present, the artificial cultivation methods of yew are mainly divided into three types: the first-breeding planting method, the tissue culture method, and the artificial cutting method. The use of pre-breeding planting method and artificial cutting method will be limited by seedling resources, coupled with low germination rate and survival rate, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 夏学云
Owner 夏学云
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