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Extraction method of taxol from branches and leaves of artificially cultivated yew

A technique for artificial cultivation of branches and leaves of Taxus chinensis, which is applied in the field of biochemical product preparation, can solve the problems of difficult separation, pollution, high toxicity of the eluent, etc., and achieves the effects of low cost, simple process and protection of plant resources.

Inactive Publication Date: 2009-10-21
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The steps of the separation process are cumbersome, and the yield of paclitaxel with a purity of more than 99% is low
Solvents, eluents, etc. are highly toxic, causing serious pollution
In addition, paclitaxel has similar properties to cephalomannine, 7-epi-paclitaxel and 7-epi-10-deacetyl paclitaxel, making it difficult to separate (Yang et al., J. Chromatogr. A, 1998, 813, 201)

Method used

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  • Extraction method of taxol from branches and leaves of artificially cultivated yew
  • Extraction method of taxol from branches and leaves of artificially cultivated yew
  • Extraction method of taxol from branches and leaves of artificially cultivated yew

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Such as figure 1 Shown, the method for extracting paclitaxel from the artificially cultivated yew branches and leaves is:

[0048] (1) Take 1 kg of dry branches and leaves of Taxus chinensis artificially cultivated, crush them with a crusher, add 3 L of 90% methanol to a 5 L round bottom flask, stir well, soak three times at 40-50 ° C, each time 4-6 Hour. Stir 3 to 6 times during the standing process. After soaking, filter to remove waste residue, and concentrate the filtrate to a light green viscous liquid with a volume of about 50ml. Add 2L of petroleum ether to the solution, mix well, add 2L of water, stir for 30 minutes, and let it stand for about 25 minutes to form a light green clear liquid in the upper layer and a dark green gelatinous liquid in the lower layer. Soluble pigments and other impurities. Add 2 L of propyl acetate to the separated lower aqueous phase, stir for 30 min, let stand for about 25 min, extract continuously for 3 times, and combine the thr...

Embodiment 2

[0056] (1) Get 100kg of fresh branches and leaves of the 3-year-old Taxus chinensis cultivated artificially, place 2m after pulverizing with a crusher 3 Then add 550L of 90% methanol, stir evenly, and soak with intermittent stirring at 40-50°C for 4 hours. After soaking, release the solution in the extraction tank, and then soak 3 times in the same way. The released methanol solutions were combined and concentrated in a concentration tank to a volume of about 25L. In the extraction tank, add about 250L of petroleum ether to the solution, mix well, add 250L of water and stir for 1 hour, let it stand for about 30min to form two layers, and separate the lower layer. Add about 250L propyl acetate to the lower layer solution, mix evenly, stir for 1 hour, and let stand for about 30min to form two layers.

[0057] (2) The propyl acetate extract of the lower layer was released, evaporated to dryness under reduced pressure, and 1032 grams of brown powder was formed, and the paclitaxe...

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Abstract

The invention relates to an extraction method of taxol from branches and leaves of artificially cultivated yew. A plurality of methods, such as planting of stocky yew, cultivating of tissue and semi-synthesis and total synthesis of taxol, have been developed home and abroad so as to obtain taxol raw material; however, the tissue cultivating method has low yield and high broth price; and the total synthesis has extremely low yield, worse therapeutic effect than natural-source taxol, and a high cost; thereby being difficult for commercial application. The invention uses branches and leaves of artificially cultivated taxus media, Yunnan taxus, southern taxus or northeastern taxus as raw materials; the raw materials are marinated by carbinol and extracted by petroleum oil and propyl acetate in sequence; and normal-pressure normal-phase silica gel column chromatography or reverse-phase high-pressure liquid chromatography is used to obtain high-purity taxol with a purity being higher than 99.6%. The preparation method has simple process, low cost, recyclable solvent and eluant; therefore, the method is applicable to industrial production and protects plant resource.

Description

technical field [0001] The content of the invention belongs to the technical field of preparation of biochemical products, and relates to a method for extracting paclitaxel from branches and leaves of yew artificially cultivated. Background technique [0002] Paclitaxel (taxol) is an antineoplastic drug with complex chemical structure and unique mechanism of action extracted from the bark, branches and leaves of Taxus (Taxaceae) plants. The sources of natural paclitaxel are very limited, mainly from yew or yew bark (Wani et al., J. Am. Chem. Soc. 1971, 93, 2325). The growth of yew tree species is extremely slow. Generally, a century-old tree can only peel 25kg of bark. If 1kg of pure taxol is extracted to provide 12,000 cancer patients, it is equivalent to the bark of 30,000 yew trees. . In order to obtain paclitaxel, methods such as planting dwarf yew trees, tissue culture, semi-synthesis and total synthesis of paclitaxel have been carried out at home and abroad to obtain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D305/14
Inventor 高锦明王俊儒张保健
Owner NORTHWEST A & F UNIV
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