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Taxus chinensis clone

A cell line and yew technology, applied in the field of yew cell lines, can solve problems such as limited sources, damage to yew resources and ecological balance, and inability to meet the growing demand for paclitaxel, shortening the production cycle and stabilizing the quality of cultures , the effect of short cultivation period

Inactive Publication Date: 2010-07-07
DALIAN PRACTICAL BIOTECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, paclitaxel is mainly obtained from bark, and the source is extremely limited, which not only destroys the resources and ecological balance of yew, but also cannot meet the growing demand for paclitaxel at all.

Method used

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  • Taxus chinensis clone

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Embodiment 1

[0020] The acquisition of embodiment 1 yew cell line

[0021] Take the natural yew seeds, first soak them in 75% alcohol for 10-30s, then sterilize them with 1% mercuric chloride solution for 8-12mins, then rinse them repeatedly with sterile water for 4-5 times, and finally put them on sterile filter paper for absorption. Moisture on dry seed surface, use sterile tweezers to inoculate solid culture in 1 / 2MS (recipe see attached table 1) with 0.5-3mg / L GA (gibberellin), 10-30g / L sucrose, 0.6g / L agar On the base, cultured at 22-27°C, the seeds can germinate and grow in about 10 days.

[0022] Get the embryos as explants, under aseptic conditions, cut the sterile seedling roots, stems, leaves, cotyledons, and embryos into 2-5mm long sections with sterile surgical scissors and sterile forceps, and inoculate them in MS (see Add 0.2mg / L-0.5mg / L 6-BA (6-benzylpurine), 2mg / L-4mg / L NAA (naphthalene acetic acid), 0.6g / L agar and 30g / L sucrose to the attached table 1) On solid medium, ...

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Abstract

The invention provides a Taxaceae clone of a stable and high-yield Taxol, in which the content of the Taxol can be 0.8-0.12% of the dry weight of the cultured materials, and is 4-6 times of wild Taxus chinensis. The invention also provides culture technique of the clone and a method for producing the clone culture in large scale rapidly. The method combines liquid and solid culture, produces seed through the liquid culture and obtains the culture materials through the solid culture, selects the culture with excellent comprehensive performance as seeds to be reserved for the liquid culture, and does the process repeatedly. The method can be used for large scale production, the production cycle is shorten greatly compared with the wild type, and has stable quality and high yield. Additionally, the culture process of the method doses not occupy cultivated land, does not damage the wild resource of the Taxus chinensis, protects the environment and solves the problem of shortage of Saussurea involucrate resource.

Description

technical field [0001] The invention relates to a yew cell line, and also relates to a preparation method of the cell line and a large-scale production method of the culture. Background technique [0002] Taxus is a class of trees and shrubs belonging to the family Taxaceae (Taxaceae) of the genus Taxus, from which the compound paclitaxel can be extracted. Paclitaxel is a diterpene alkaloid, which has been clinically proven to be effective in the treatment of ovarian cancer, lung cancer, colon cancer, metastatic breast cancer, melanoma, leukemia and other cancers, making paclitaxel one of the most popular anticancer drugs currently one. [0003] At present, paclitaxel is mainly obtained from bark, and the source is extremely limited, which not only destroys the resources and ecological balance of yew, but also cannot meet the growing demand of people for paclitaxel. Therefore, the application of cell culture to the development of Taxus chinensis can meet the clinical deman...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12R1/91
Inventor 刘汉石
Owner DALIAN PRACTICAL BIOTECH
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