The invention discloses a
copper metal organic framework (Cu-MOFs) mimic
enzyme material as well as preparation and application thereof. The
nanotopography of the
copper metal organic framework mimicenzyme material is in a flake shape. The Cu-MOFs mimic
enzyme material can be prepared by the following steps: dissolving
cupric nitrate into N,N-
dimethylformamide to obtain a solution A and dissolving 5-nitroisophthalic acid into the N,N-
dimethylformamide to obtain a solution B; adding the solution A and
methanol into the solution B under the stirring condition, stirring for 20 to 40 minutes, putting a stirred solution in a reactor, and carrying out thermostatic reaction at the temperature of 70 to 80 DEG C for 48 to 72 hours; after the reaction is ended, centrifugally separating, washing, and then
drying at 60 DEG C for 12 to 24 hours to obtain a flaky Cu-MOFs mimic
enzyme material; dispersing the flaky Cu-MOFs mimic enzyme material into water to obtain a Cu-MOFs mimic enzyme solution. The material has excellent activity of mimetic
peroxidase; compared with natural
horse radish peroxidase (HRP), the material has the advantages of simple preparation process, low cost, good adaptability and the like; the material can be used as a substitute of the HRP, and has potential application value in aspects of bioinstrumentation,
immunoassay and
clinical diagnosis.