The invention discloses a fruit and vegetable grain fermentation beverage. The fruit and vegetable grain fermentation beverage comprises the following raw materials in parts by weight of drinking water, fruits, rhizoma polygonati odorati, Chinese wolfberry fruits, poria cocos, Chinese jujubes, mulberries, haws, raspberries, coix seeds, malt, Chinese yam, licorice roots, rhizoma polygonati, fresh reed rhizomes, lophatherum herbs, chrysanthemums, gordon euryale seeds, black fungi, white fungi, tea leaves, black rice, buckwheat, brown granulated sugar, honey and lactic acid bacteria, wherein foodadditives can be added or cannot be added. The preparation method of the fruit and vegetable grain fermentation beverage comprises the following preparation steps of performing pretreatment, preparing pulp, performing decoction, performing fermentation, performing separation and filtration, performing mixing, performing filling, performing sterilization and performing dispatching from the factoryafter the products are qualified through quality detection. The formula has the effects of complementing cellulose, vitamins and minerals, protecting cells, regulating microbial population in bodiesbalanced, regulating blood sugar, blood pressure and blood fat balanced, protecting internal organs, promoting nutritive absorption, eliminating toxic substances in intestines, preventing obesity, preventing dental caries, resisting oxidation, prevention tumors, delaying ageing and the like.