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Novel purification method of lycium chinense polysaccharide

A technology of Lycium barbarum polysaccharides and a new method, which is applied in the field of purification of Lycium barbarum polysaccharides and Lycium barbarum polysaccharides by macroporous adsorption resin-silica gel chromatography coupling method, can solve the problems of high process cost, separation, and poor application effect, and achieve anti-pollution Strong ability, obvious separation effect and fast exchange speed

Inactive Publication Date: 2013-04-10
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction process of Lycium barbarum polysaccharides in the above-mentioned patent documents is mainly refined through the steps of raw material pretreatment, ultrasonic-assisted extraction, and alcohol precipitation separation. Far inferior to the separation effect of macroporous adsorption resin coupled with silica gel chromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Weigh 10 kg of Lycium barbarum fruit, crush it into a coarse powder, add 18 times the amount of 90% methanol to reflux and extract for 140 minutes, and filter; the filtrate is recovered from methanol, concentrated to 4 Baume, and concentrated with hydrochloric acid:water solution with a volume ratio of 1:1. The pH value of the liquid was adjusted to 2.8, left for 40 minutes, and filtered; the filtrate was placed on a HPD100 macroporous resin adsorption column, and the impurities were washed with water after adsorption, and then washed with 15% methanol, and then eluted with 70% methanol, polysaccharides The semi-finished product eluent, the polysaccharide semi-finished product eluent is recovered from methanol, concentrated and dried polysaccharide semi-finished product; the resulting polysaccharide semi-finished product is put on a silica gel chromatography column, and the mixture of acetone and methanol with acetone:methanol volume ratio of 2:1 washes impurities, and th...

Embodiment 2

[0022] Weigh 15 kg of Lycium barbarum fruit, crush it into coarse powder, add 20 times the amount of 95% methanol to reflux and extract for 160 minutes, and filter; The pH value of the solution was adjusted to 3, left for 40 minutes, and filtered; the filtrate was placed on a HPD400 macroporous resin adsorption column, and the impurities were washed with water after adsorption, and then washed with 10% methanol, and then eluted with 60% methanol, polysaccharides Semi-finished product eluent, polysaccharide semi-finished product eluent recovers methanol, concentrates and dries the polysaccharide semi-finished product; puts the obtained polysaccharide semi-finished product on a silica gel chromatography column, and washes impurities with a mixture of acetone and methanol with a volume ratio of acetone:methanol of 1:1, and then washes the impurities with Acetone:methanol volume ratio of 7:1 acetone and methanol mixture elution, polysaccharide eluent 3.5L; add 140g injection of act...

Embodiment 3

[0024] Weigh 20 kg of Lycium barbarum fruit, crush it into a coarse powder, add 15 times the amount of 87% methanol to reflux and extract for 120 minutes, and filter; The pH value of the solution was adjusted to 2.5, left for 40 minutes, and filtered; the filtrate was placed on an AB-8 macroporous resin adsorption column, and the impurities were washed with water after adsorption, and then washed with 5% methanol, and then eluted with 80% methanol. The polysaccharide semi-finished product eluent, the polysaccharide semi-finished product eluent is recovered from methanol, concentrated and dried polysaccharide semi-finished product; the gained polysaccharide semi-finished product is put on a silica gel chromatography column, and the mixture of acetone and methanol with acetone:methanol volume ratio of 3:1 washes impurities, Then use acetone:methanol volume ratio of 5:1 mixture of acetone and methanol to elute, polysaccharide eluent 5L; add 200g injection of activated carbon, stir...

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Abstract

The invention discloses a novel purification method of lycium chinense polyhexose, which comprises the steps that lycium chinense fruits are smashed into crude powder, 15 times of 85% methanol is added for reflux extraction for 120min and filtered; methanol is recovered from a filtrate; the filtrate is concentrated to 3 Be; hydrochloric acid and water at a volume ratio of 3:1 are used to regulate a pH value of a concentrated solution to 2.5; the concentrated solution is placed for 20 min and filtered; a broad-spectrum macroporous resin adsorption column is placed in the filtrate for collection of an eluent; methanol is recovered; the eluent is concentrated and dried to form a polysaccharide semifinished product; a silica gel chromatographic column is placed in the polysaccharide semifinished product; acetone and carbinol at a volume ratio of 3:1 are used to wash impurities; acetone and methanol at a volume ratio of 7:1 are used for elution; an eluent is collected; the collected polysaccharide eluent is refined to form the lycium chinense polysaccharide finished product; and the content is over 70%. The novel purification method has the greatest characteristics that with the adoption of a macroporous adsorption resin-silica gel chromatography coupled method, the novel purification method is quick and convenient; sources of raw materials are abundant; the manufacturing cost is low; the separating effect is obvious; and the extraction purity is high.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for purifying wolfberry polysaccharide, in particular to a new method for purifying wolfberry polysaccharide by using a macroporous adsorption resin-silica gel chromatography coupling method. Background technique: [0002] Lycium chinense, scientific name: Lycium chinense, alias Gouqizi, wolfberry red fruit, beet seed, wolfberry, dog milk, red green pepper, wolfberry, wolfberry fruit, ground bone, wolfberry eggplant, red earrings, blood wolfberry, wolfberry bud Lycium barbarum, wolfberry bean, blood wolfberry, and wolfberry are the fruits of the plant Lycium barbarum of the genus Lycium barbarum in the family Solanaceae. Contains betaine (betane), atropine (aTCMLIBopine), gyoscyamine (gyoscyamine), wolfberry polysaccharides, and also contains zeaxanthin, physalis physene, cryptoxanthin (cryptosxanthin), scopolamine (scopo-letin), carrot Vitamin C, thiamine, ribof...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 姚德坤姚德利张亚红姚航黄运生吴海龙
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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