71 results about "Genome research" patented technology

A system, method and program product, the method comprising, in one embodiment, providing a secure testing service for patient's identification and payment data encrypted at the data level, non-identifiable method for a patient to have a genetic tests to identify variants or mutations of their genes or combinations of genes that predispose the patient to develop or have an identified disease, comprising: obtaining electronically genomic information for a patient comprising at least one of, (a) DNA information, (b) RNA information, (c) complementary DNA or RNA information, (d) transfer RNA (tRNA) information (e) messenger RNA (mRNA) information, and (f) Expressed Sequence Tags (EST) to identify an abnormal gene; searching by one or more computers electronic databases using the identified abnormal gene to obtain genetic sequencing and basic research, patient predispositions, and pharmacognetics that predict the response and reaction of patients with identified genetic abnormalities related to the identified abnormal gene and individual medications that may be prescribed relating to the identified abnormal gene or a relationship with said identified abnormal gene; performing an update search on at least a periodic basis to learn about subsequent genomic research developments and treatments for the identified abnormal gene, specific genes with variants or mutated genes identified in the genetic test; sending electronically via an Internet communication link data comprising or derived from the searching step and the update search to the patient or a third party; and with the sending step performed using a privacy component that prevents transmission to any third party unless predetermined permission clearance data is in the system.

The invention belongs to the field of marine fish mitochondrial genome research, and particularly relates to a marine fish mitochondrion 12S rRNA (ribosomal ribonucleic acid) gene amplification primer. The primer is composed of two single-chain oligonucleotide chains, wherein a light chain primer is a nucleotide sequence shown as SEQ ID NO.1, and a heavy chain primer is a nucleotide sequence shown as SEQ ID NO.2. The invention also provides a design method of the amplification primer and a method for amplifying a marine fish DNA (deoxyribonucleic acid) solution by using the amplification primer. The invention can efficiently and specifically amplify multiple marine fish mitochondrion 12S rRNA genes and can be used for the analytical study on the system evolution of different taxonomic categories of fishes, thereby providing a powerful tool for fish species identification, germplasm resource survey and system evolution research.

The invention relates to a method for using rice auxin to induce protein genes to be cloned, which is characterized in that the implementation steps comprise: (1) the preparation of a rice transformation receptor; (2) the genetic transformation of the rice; (3) the screening of kanamycin-resistant callus tissue and the regeneration of plants; (4) the screening of the mutant of a T-DNA inserted progenies; (5) Tail-PCR; (6) the comparison and analysis of sequences on the Internet. In the invention, a rice mutant with a short plant height is obtained when carrying out rice functional genome research by using T-DNA label method; the lateral neighboring sequences of the mutant are researched by using TAIL-PCR technology; meanwhile, the position where the mutant T-DNA inserts the rice genome is arranged on the No. 4 chromosome of the rice by the comparison on the databases of NCBI and TIGR on the Internet; moreover, the rice BAC clone (OSJNBa0084K01) of the position is found out. The T-DNA is inserted between the two genes of the clone by analyzing the clone. Known functional genes with a very high homology with BAC cloning code amino acid sequence are forecasted by the sequence comparison on the Internet.

The invention discloses a bacillus gene traceless knockout / knockin plasmid and method, and a kit. The genome of the bacillus gene traceless knockout / knockin plasmid contains only one kind of resistance gene marked with a positive selection marker, a replicon capable of being duplicated in escherichia coli, a thermo-sensitive type replicon capable of being duplicated in bacillus, at least one I-Scel enzyme cutting site and only one kind of chromogenic protein gene, wherein promoters in front of the resistance gene and the chromogenic protein gene are both constitutive promoters in bacillus. The invention further provides a helper plasmid used for improving the knockout / knockin efficiency of the bacillus gene traceless knockout / knockin plasmid, and resistance gene and chromogenic protein gene in the genome of the helper plasmid are different from those of the knockout / knockin plasmid. By the adoption of the two plasmids, one or more target DNA sequences in the genome of bacillus can be modified continuously or iteratively, and the plasmids can be applied to multiple fields including bacillus genetic modification, metabolic process researching, functional genome researching and industrial application researching.

The invention provides a method for identifying indica rice and japonica rice varieties via metabolite content, specifically identifying indica rice and japonica rice varieties via the weight percentage ratio of asparagine to alanine in rice seeds. The weight percentage ratio of asparagine to alanine in seeds of indica rice variety Guang-Lu-Ai No.4 is used as a reference value, in the same test conditions, if the weight percentage ratio of asparagine to alanine in seeds of to-be-tested variety is larger than the reference value, the to-be-tested variety is judged to be indica rice, and if the weight percentage ratio of asparagine to alanine in seeds of to-be-tested variety is smaller than the reference value, the to-be-tested variety is judged to be japonica rice. The method provided by the invention is the first proposal for identifying indica rice and japonica rice varieties via the weight percentage ratio of asparagine to alanine in rice seeds, plays an important guiding role in rice functional genome research and indica / japonica rice differentiation research, and can also promote the scientific research in indica / japonica rice cross breeding and environmental adaptation of varieties.

The invention discloses a pseudomonas donghuensis 22G5 and an application of the pseudomonas donghuensis 22G5 in prevention and treatment of crop verticillium wilt. The pseudomonas donghuensis 22G5 ispreserved in the China General Microbiological Culture Collection Center on July 08, 2019, and a preservation number of the pseudomonas donghuensis 22G5 is CGMCC No. 18084. The pseudomonas donghuensis 22G5 has no pathogenicity to plants, has a remarkable inhibition effect on cotton verticillium wilt caused by Verticillium dahliae, can remarkably reduce the occurrence of cotton verticillium wilt,and has a very good biological control effect. The genome studies show, the pseudomonas donghuensis 22G5 has a gene cluster for synthesizing gene cluster of siderophore 7-hydroxycycloheptyltrienol ketone, and the compound can be used as an iron ion chelating agent to inhibit the iron element necessary for the growth of verticillium dahliae, so that the growth of pathogenic bacteria is inhibited. The strain can be used for developing biopesticides for crop verticillium wilt, and provides a new thought and a new method for biological prevention and treatment of crop verticillium wilt.

The invention discloses a method for extracting mitochondria and mitochondrial protein from cotton. The method has minimum mechanical damage to mitochondria, nucleus components and plasmid components except mitochondria as well as phenolic and polysaccharide substances can be removed effectively, the contamination rate is reduced, the purity and the quality of mitochondrial protein are improved, experimental technique requirements of cotton genome research, mitochondrial proteome research, metabolism regulation network research and the like are met, and related research work fields are broadened.

The invention discloses Pseudomonas protegens XY2F4 and application thereof in control of crop verticillium wilt. The Pseudomonas protegens XY2F4 was collected in the China General Microbiological Culture Collection Center (CGMCC) of the China Committee for Culture Collection of Microorganisms (CCCCM) on June 24, 2019, with the collection number of CGMCC No.18017. The pseudomonas protegens XY2F4 has no virulence for plants, has a significant inhibition function on cotton verticillium wilt caused by verticillium dahliae, can remarkably relieve occurrence of cotton verticillium wilt and has goodbiological control effects. Genome research shows that the Pseudomonas protegens XY2F4 has a series of biological control related compound synthesis gene clusters, can be used for developing biological pesticides for crop verticillium wilt diseases, and provides a new thought and a new method for biological control of the crop verticillium wilt.

The invention relates to the field of biological genome research and specifically discloses a primer special for D-loop region complete sequence sequencing of culter erythropterus and an application. According to the invention, a degenerate primer is adopted for performing PCR amplification and then a special primer is used for sequencing. A D-loop complete sequence sequencing method provided by the invention has the advantages that cost is low, mastering is easy and the complete sequence can be acquired by once positively and negatively sequencing, so that an efficient experimental technical support is supplied for geographical population identification of culter erythropterus, research on molecular genetics and molecule system evolutionism and early resource identification and the enlightenment is supplied for the other animal experiments.

The invention relates to the research field of cephalopod mitochondrial genome, and especially relates to a portunidae mitochondrial COI gene universal primer, and design and amplification methods thereof. The universal forward primer of mitochondrial COI gene of Portunidae is SEQ ID NO. 1: 5 '- TCNACAAAYCATAAAGAYATYGG-3 ', and that reverse prim is SEQ ID NO. 2: 5'-TANACYTCWGGRTGHCCRAARAAYCA- 3'.The present invention solves the problem that the prior art does not utilize the DNA barcode technology to identify the family Portunidae, and has the following advantages: 1) the universal primer ofmitochondrial COI gene of the family Portunidae provided by the present invention can amplify a plurality of families of Portunidae in batch at one time with high efficiency, and can provide great convenience for the species identification of the family Portunidae; 2) design universal primers in two conservative region of mitochondrial COI gene of family Portunidae to amplify an intermediate mutation sequence, that amplification length is about 600-700 bp, sufficient information sites are used for identification and differentiation of physical evidence, and a sequencing reaction can be complete, the operation is simple, and the cost is saved.

The invention relates to a fish transgenosis breeding method which belongs to the technical field of molecule biology fish breeding. The method mainly comprises the following steps: preprocessing the genome DNA of a fish and obtaining a DNA rearrangement fragment distinguished from the original genome DNA; guiding the DNA rearrangement fragment into an oocyte of an original fish by a transgenosis method to achieve the function with the genome of the original fish; forming a variable gene and generating individuals with mutational appearance; screening the individual with favorable production characteristics and carrying out further breeding. The invention judges whether the DNA rearrangement fragment enters the genome of the original fish or not by a TRAP method, obtains the variable gene by a two-step amplification method, provides a base for carrying out functional genome research later and obtains a unique molecular mark. The method is simple and can be widely applied to the breeding of fishes, other animals and plants, and the like and the research of relevant functional genomes.

The invention discloses a method for building gramineae general molecular marker CNS-AFLP and developed primer sequence with good universality in gramineae crop, which is applicable to the technical fields of genomics, molecular biology and bioinformatics. Coding sequence or EST is taken as a template so as to design the primer; and conserved non-coding sequence (CNS) is taken as the template so as to design the primer. Detecting polymorphism is base sequence length and restriction enzyme cutting site variation of non-coding region between two CNSs and between CNS and Exon. The invention comprises steps of CNS developing, primer designing, PCR amplifying, detecting the polymorphism of amplified fragment, preparing and analyzing the polymorphism of restriction enzyme cutting fragment, etc. Compared with SSR mark, ILP mark and EST mark, the invention is characterized in that transspecies application range is wider and the designed primer is applicable to various species in the gramineae, thereby improving research efficiency. The method is a molecular marking method based on PCR technology, so that the designed primer has high universality and wide transspecies application range, thus effectively promoting the relatively laggard gramineae crop genome research and cereal crop comparative genetics.

The invention belongs to the fish mitochondrial genome research field, and concretely relates to a COIII and ND3 gene amplimer, the gene amplimer is composed of two strips of single chain oligonucleotide chains, wherein a light chain primer is a nucleotide sequence shown in SEQ ID NO.1, and a heavy chain primer is a nucleotide sequence shown in SEQ ID NO.2. The invention also provides a design and an amplification method of the amplimer. The invention can specifically amplify a plurality of goby mitochondrion COIII and ND3 gene sequences with high efficiency, and can be used in goby different classification category systems evolution and classification research.