Method for identifying indica rice and japonica rice varieties via metabolite content

An indica rice variety and metabolite technology, applied in the field of biological identification, can solve problems such as differences in the content of asparagine and alanine in japonica indica rice, and achieve the effects of promoting environmental adaptation, short time consumption and convenient operation.

Active Publication Date: 2014-01-15
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0004] However, so far, no one has found a significant difference in the ratio of asparagine to alanine content in japonica-indica rice varieties, and used this feature to distinguish japonica-indica rice

Method used

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Embodiment

[0024] The present embodiment adopts the method of GC-MS to detect the weight percentage content of asparagine and alanine in the rice seed sample, specifically see Table 1; Find the weight percentage content of asparagine and alanine between japonica rice and indica rice varieties There were significant differences in the ratio, and Guangluai 4 was selected as the critical value control variety.

[0025] Table 1 The ratio of the type of experimental material used in the embodiment and the weight percent content of asparagine and alanine

[0026] serial number

rice variety

source

Subspecies

Asparagine / Alanine

1

Yangdao No. 6 / 9311

Jiangsu

Indica

15.32

2

Qing Er Ai

Guangdong

Indica

12.69

3

bantam nantes

Guangdong

Indica

12.18

4

Tesanai No. 2

Guangdong

Indica

11.37

[0027] 5

Ye Qinglun

Guangdong

Indica

11.19

6

Xiangwa...

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Abstract

The invention provides a method for identifying indica rice and japonica rice varieties via metabolite content, specifically identifying indica rice and japonica rice varieties via the weight percentage ratio of asparagine to alanine in rice seeds. The weight percentage ratio of asparagine to alanine in seeds of indica rice variety Guang-Lu-Ai No.4 is used as a reference value, in the same test conditions, if the weight percentage ratio of asparagine to alanine in seeds of to-be-tested variety is larger than the reference value, the to-be-tested variety is judged to be indica rice, and if the weight percentage ratio of asparagine to alanine in seeds of to-be-tested variety is smaller than the reference value, the to-be-tested variety is judged to be japonica rice. The method provided by the invention is the first proposal for identifying indica rice and japonica rice varieties via the weight percentage ratio of asparagine to alanine in rice seeds, plays an important guiding role in rice functional genome research and indica / japonica rice differentiation research, and can also promote the scientific research in indica / japonica rice cross breeding and environmental adaptation of varieties.

Description

technical field [0001] The invention belongs to the technical field of biological identification; in particular, it relates to a method for identifying japonica and indica rice varieties by using the content of seed metabolites. Background technique [0002] Rice is the staple food of more than 50% of the world's population. Cultivated rice was domesticated from a common ancestor, wild rice (Oryza rufipogon). Under the dual effects of natural selection and artificial selection, cultivated rice presents thousands of varieties with significant genetic differences. At present, there are two main subspecies of cultivated rice, japonica rice (Oryza sativa L. subsp. Japonica Kato) and indica rice (Oryza sativa L. subsp. Indica Kato). There is obvious reproductive isolation between typical japonica rice and indica rice. Generally speaking, japonica rice varieties are suitable for planting in temperate regions at high latitudes or high altitudes, while indica rice varieties are sui...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 张大兵石建新胡朝阳
Owner SHANGHAI JIAO TONG UNIV
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