Primer special for D-loop region complete sequence sequencing of culter erythropterus and application

Abstract

The invention relates to the field of biological genome research and specifically discloses a primer special for D-loop region complete sequence sequencing of culter erythropterus and an application. According to the invention, a degenerate primer is adopted for performing PCR amplification and then a special primer is used for sequencing. A D-loop complete sequence sequencing method provided by the invention has the advantages that cost is low, mastering is easy and the complete sequence can be acquired by once positively and negatively sequencing, so that an efficient experimental technical support is supplied for geographical population identification of culter erythropterus, research on molecular genetics and molecule system evolutionism and early resource identification and the enlightenment is supplied for the other animal experiments.

Description

technical field [0001] The invention relates to the field of biological genome research, in particular to a special primer for full-sequence sequencing of the D-loop region of carps and its application. Background technique [0002] Chrysalis is a famous and high-quality characteristic fish in freshwater culture in my country, which mainly includes chrysalis, Mongolian chrysalis, Daphne chrysalis, red-finned chrysalis and other chrysalis subfamily fish. Carp is widely distributed in large rivers, lakes and reservoirs in China. In recent years, with the breakthrough and gradual improvement of the artificial breeding and breeding technology of Chinese carp, its breeding scale has continued to expand, but so far, all the carp as parents in production come from natural populations that have not been genetically selected, and long-term inbreeding has caused Germplasm degradation. Moreover, due to the high-density artificial breeding and breeding, the growth rate of chrysalis sl...

AI Technical Summary

Problems solved by technology

Although there are domestic and foreign literatures using the hypervariable region of the D-loop region to study the genetic diversity and phylogenetic evolution of fish, the genetic information contained in the full sequence is more abundant

At present, there is no systematic and comprehensive study on the genetic structure assessment and early resource identification of local species of Chinese carp by using the full sequence of the D-loop region. Therefore, it is very necessary to establish a method for the full sequence amplification and sequencing of the mitochondrial D-loop region of the carp

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