Microdrop oscillatory silicone groove polymeraxe chair reaction biological chip

Abstract

The present invention relates to a microdrop shaking silicon groove type PCR biological chip, including microgroove and thermostatic region positioned in the back surface of microgroove and separatedby heat-insulating groove, resistive microtemperature sensor positioned on the back surface of the microgroove and microheaters which are symmetrically placed at two sides of said temperature sensor.The biological chip has extensive application in the fields of biological genome research, forensic medicine, genetic analysis, therapeutic effect evaluation and medical diagnosis, etc.

Description

technical field [0001] The invention belongs to the field of medical instruments, in particular to the design of a nucleic acid PCR clinical diagnosis biochip suitable for high throughput, rapidity and automation. Background technique [0002] Polymerase chain reaction (PCR, Polymerase Chain Reaction) is one of the important means of modern nucleic acid analysis. It can amplify the number of target nucleic acid molecules nearly doubled in one cycle, and after 20-30 cycles, the limited nucleic acid The molecule was amplified 109 times, thereby achieving the enrichment of the target molecule and preparing for further analysis. The birth of the PCR process has greatly accelerated the process of research on the genome structure of various organisms, and has a wide range of applications in forensic science, genetic analysis, treatment effect evaluation, and medical diagnosis. [0003] In recent years, with the development of micro-processing technology, microchips used in clinic...

AI Technical Summary

Problems solved by technology

The disadvantages of this micro-PCR chip are: 1) it is not based on silicon micro-processing technology, and is not compatible with IC technology, so that the chip needs relatively complex and huge external equipment to provide control and communication, etc., which is difficult to produce in large quantities at low cost; 2) the structure Large size, long time to complete the PCR process; 3) The PCR operation in each channel of the same array needs to have the same denaturation, annealing and extension temperature; 4) It is difficult to realize automatic control and low efficiency

The disadvantages of this micro-PCR chip are: 1) the chip is close to the airtight microcavity, and during the PCR denaturation process, since the temperature is close to the boiling point of the working fluid, bubbles are easily generated and the chip structure is destroyed; 2) it is not based on microfluidic technology, so it is difficult Integration with other micro-devices constitutes a real lab-on-chip (Lab-on-Chip); 3) The chip uses external fluid (air) to achieve the cooling process, and the cooling rate is low

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