38 results about "Genomic research" patented technology

Methods of doing business and systems for implementing those methods which improve the effectiveness and success of the research and development of technology such as pharmaceuticals, biotechnology, agrochemicals, medical technology, and genomics. The methods include the application of value and cost modeling methodologies to provide a pharmaceutical, biotechnology or genomics research and development organization with information and data which will assist it in making choices and decisions about its prospective products and the manner of research and development thereof in order to realize the greatest economic gain from commercialization of the products.

The invention belongs to biotechnology field, and relates to a method for developing a dendranthema SSR (Simple Sequence Repeat) primer based on transcriptome sequencing. A lot of sequence information is processed in batch for searching an SSR sequence and designing an SSR labeled primer based on the transcriptome sequencing by utilizing EST-SSR (Expressed Sequence Tags-Simple Sequence Repeat) interspecific metastasis and using a Perl (Practical Extraction and Reporting Language) programming language method in combination, so that the defects of low efficiency, long time consumption, high cost and the like of the SSR development are conquered. Different dendranthema materials are selected for verifying the designed SSR primer, and the primer is a successful SSR primer if detected out by any strip. By adopting the method, 1788 pairs of SSR primers are successively designed, so that a novel method and thinking are provided for the development of the dendranthema SSR primer to further achieve molecular marker assistant selection breeding and comparative genomics research.

The invention discloses a method for monitoring Siniperca chuatsi germplasms by virtue of microsatellite genetic marker. The method comprises genome DNA extraction and detection, microsatellite sequence search and analysis, polymorphic microsatellite primer selection and optimization, polymorphic microsatellite detection and monitoring of different geographic siniperca chuatsi populations and hereditary features thereof. Microsatellite loci selection and polymorphic microsatellite marker development and verification are performed on a large number of EST generated by siniperca chuatsi transcriptome sequencing, so that 15 EST-SSR polymorphic microsatellite markers are developed, and different geographic siniperca chuatsi populations and hereditary features thereof can be monitored; the limitations of fuzzy genetic background and less molecular marker amount in existing germplasm detection, selective breeding and germplasm improvement research of siniperca chuatsi can be broken, and a basis is provided for germplasm detection, selective breeding and germplasm improvement of siniperca chuatsi, so that germplasm detection, auxiliary genetic breeding and genomic research processes of siniperca chuatsi molecular markers are promoted.

The invention discloses a lilium regale wilson LrPAL-1 gene and application thereof. The nucleotide sequence of the LrPAL-1 gene is as shown in SEQ ID NO.1 and the coded amino acid sequence of the LrPAL-1 gene is as shown in SEQ ID NO.2. The overexpression vector containing the LrPAL-1 gene is transferred into tobacco, the lignification degree of an obtained transgenic tobacco plant is increased,the lodging resistance of the plant is improved, and particularly, the upright state of fresh cut lily flowers can be maintained. The transgenic plant has an obvious inhibition effect on pathogenic bacteria botrytis cinerea and alternaria alternata. Meanwhile, the functional genomics researches prove that the lilium regale wilson LrPAL-1 gene has an important biological function of changing the morphology of plant leaves. The invention provides an important target for molecular genetic improvement of lilium plants; the lilium regale wilson LrPAL-1 gene has an important value and significance for cultivation and production of new varieties of plants with ornamental value and landscape ecological value; and a technical support is provided for cultivation of new lilium germplasm with dual values of lodging resistance and pathogenic bacteria resistance.

The invention discloses a method for inducing haploid endosperm callus tissues by using ripe taxales chinensis var.mairei endosperms, and the method comprises the following steps of: (1) seed exotesta peeling: mechanically breaking a shell, and manually peeling exotesta; (2) explants sterilization: sequentially sterilizing by using 70% alcohol for 90 seconds and 0.1% mercuric chloride for 12 minutes, and washing five times with sterile water for later use; (3) seed pretreatment: sealing with the sterile water, and soaking seeds for 1-5 days at normal temperature; (4) separation of endosperm tissues; and (5) in vitro induction of endosperm callus tissues: inoculating seed endosperms to a callus tissue-inducing culture medium, so as to obtain the 97.92% induction rate after 30 days. By utilizing the method, ripe taxales chinensis var.mairei endosperm callus tissues can be obtained conveniently and quickly, and the ripe taxales chinensis var.mairei endosperm callus tissues lay a foundation for simplifying genetic analysis and chromosome operation and are used as transgenic materials and genomics research materials.

The invention relates to a new method for high-efficiently capturing a chromatin nucleosome vacancy district at a whole-cell level. The new method can locate the chromatin nucleosome vacancy district more accurately and sensitively in the range of a complete genome. The method for capturing the chromatin nucleosome vacancy district at a high-throughput complete genome level can be used to search the chromatin nucleosome vacancy district of the transcriptional regulation of eukaryotic cells, and is an effective means for functional genomics research.

The invention provides an activating tag Ac/Ds transposons system. The system consists of a transposase Ac expression vector and a transposons Ds expression vector. The system is characterized in that the transposons Ds expression vector is provided with an activating tag, the activating tag refers to four 35S enhansers which are connected with one another in series, and the four 35S enhansers are arranged at the inner side of the Ds 3'end. After the Ac/Ds transposons system provided by the invention is used, an Ac transposons gene can be expressed in a plurality of plants at a high level, the expression level of a transposons insertion site which is near to the gene can be activated at the high level, a dominant mutant can be created, and the flanking sequence of the transposons insertion site can be identified and separated with high efficiency for building a mutant library of a polyploid plant. The activating tag Ac/Ds transposons system provided by the invention is an ideal plant functional genomics research tool, and has an important meaning on building the mutant of crops especially for the polyploid plant, and researching the functional genomics.

A method of preparing a Chinese cabbage mutant by means of isolated microspore culture and EMS mutagenesis is disclosed. In a process of isolated microspore culture, microspores are subjected to mutagenesis treatment by utilization of an EMS solution, and a mutant is screened from double-haploid plants obtained by culture and is identified. The method is aimed at rapid preparation of a homozygous Chinese cabbage mutant, provides a novel germplasm resource for breeding research of Chinese cabbage, and provides a mutant material for functional genomics research of Chinese cabbage.

The invention belongs to the technical field of propagation of pears and particularly discloses an adventitious-bud-grafted rapid propagation method for pears. According to the method, annual or biennial Pyrus betulaefolia bunge or Pyrus calleryana solid-wood seedlings are selected as rootstocks, adventitious buds obtained through tissue culture and subculture propagation of Pyrus sinensis or goldpears are selected as scions, the rootstocks and the scions are pretreated before grafting, then, grafting is carried out in a greenhouse, T-shaped bud grafting is adopted as a grafting method, basalportions of adventitious-bud scion stems are beveled, then, the beveled basal portions of the adventitious-bud stems are dipped with a hormone mixed solution, the dipped adventitious-bud stems are inserted into T-shaped cuts of rootstock stems, grafted ports are subjected to sealing and plastic bag covering treatment, and then, grafted post-management is carried out. According to the adventitious-bud-grafted rapid propagation method for the pears, provided by the invention, culture is carried out in the greenhouse, the operation is simple, the culture period is short, the survival rate is high, the problem that adventitious roots of the pears are difficult in regeneration can be effectively solved, and a technical support is provided for tissue culture regeneration, industrialized seedling rearing and functional genomics research of the pears.

The invention discloses a method for gene gun-mediated genetic transformation of moso bamboos. The method includes the steps: moso bamboo mature embryo acquisition and pre-culture; extraction of plasmid containing target genes; gene gun parameter setting; resistance screening; transformed plant identification; positive plant culture; transplantation. The method further includes a formula of a special culture medium in processes of transformation and induction. The method solves the problem of difficulty in genetic transformation of the moso bamboos, the period of acquiring transgenic bamboos can be effectively shortened (resistant seedlings are acquired after screening is performed for one month, and positive plants can be acquired for two to four months), and the probability of acquiring the transgenic bamboos is increased. The method is good in repeatability, plays an important role in industrial bamboo genetic improvement, bamboo function genomics research and the like, and has a wide application prospect.

The invention specifically relates to a method and medium formula capable of increasing the sprouting ratio of immature wheat embryos, belonging to the field of plant transformation. The invention provides an induction medium MSV4 for the embryonic calluses of the immature wheat embryos. The induction medium MSV4 can improve the induction rate of the embryonic calluses of the immature wheat embryos and is characterized by comprising glycine with a final concentration of 0.027 mM, glutamine with a concentration of 0.1 to 2.57 mM, casein hydrolysate with a concentration of 50 to 500 mg/L and Dicamba with a concentration of 0.5 to 52 mg/L. A high-efficiency wheat embryo cultivation system is established in the invention and is of great significance to subsequent functional genomics research on wheat and molecular breeding of wheat.

The invention provides a long double-stranded RNA (ldsRNA) expression vector for silencing a whole genome or across the genome. The vector is a plant binary expression vector integrated with the following sequences: a terminator-a promoter-an LoxP site sequence-an attP1 site sequence-a ccdB gene-a CmR gene-an attP2 site sequence-the LoxP site sequence-the promoter-the terminator. The vector employs conjugated promoter sequences between T-DNA border sequences, the LoxP site sequence and the attP site sequences to integrate a plant or an insect cDNA library into the vector through an enzyme digestion reaction or a recombination reaction. Plant genes are silenced in a plant by an RNA interference (RNAi) or genes of diseases and insect pests are silenced by feeding, and thus the vector is applied to a plant functional genomic research for discovering new gene functions or screening target genes for host-delivered RNAi, and thereby cultivating broad-spectrum or specific new varieties with disease and pest resistance.

The invention discloses a Tripterygium wilfordii protoplast separation and instantaneous conversion method. Experiments prove that the yield of protoplasts obtained by the separation method is 4.24*10<5>/g FW, and the activity is 94.5%; and exogenous plasmids are successfully converted into the protoplasts extracted by the invention through a PEG 4000 mediated conversion method and are smoothly expressed, thereby successfully establishing a Tripterygium wilfordii suspension cell protoplast instantaneous conversion system. The invention lays a foundation for carrying out functional genomics research of Tripterygium wilfordii in a deep-going way, and simultaneously provides a material basis for research of Tripterygium wilfordii such as gene editing, subcellular localization, gene overexpression and interference and the like.

This application relates to molecular biology, and more specifically to a method which uses the molecular recognition between a target molecule ligand and an aptamer, magnetic separation and MS qualitative and quantitative analysis to enable the association between the detection of multi molecules and information of multiple functional groups, and effectively determine the correlation between molecules and functional groups of the body or tissue. This application can easily purify the target molecules by magnetic separation and can effectively obtain the target molecule group based on the high specificity and affinity of the aptamer. In addition, based on the MS detection, this application can effectively perform the qualification and quantification of the multi molecules, achieving the secondary molecular detection and improving the detection accuracy. The simultaneous qualification and quantification of multi molecules can not only accurately reflect the relationship among molecules, but also reveal the interrelationship among body functions, playing a significant role in the proteomics and genomics research and clinical molecular detection.

The invention discloses an establishing and screening analysis method for a panicum miliaceum ethylmethylsulfone (EMS) mutant library, and belongs to the field of agricultural breeding. According to the method disclosed by the invention, a technology platform of panicum miliaceum EMS mutagenesis is constructed by screening of an optimal concentration of EMS-processed Yixuan red panicum miliaceum seeds; 103 mutant plant lines are found after obtained 2333 M2-generation plant lines are planted, morphological characters include a leaf color, a leaf type, a plant height, fertility, an ear type, anear color and a grain color, and the mutation frequency is 4.4%; and the mutant library with abundant phenotypes is established, and the mutant library can provide abundant mutant resources and novelgermplasm for functional genomic research and breeding of the panicum miliaceum.

The invention discloses a method for inducing haploid callus by utilizing actinidia melanandra anther. The method comprises the following steps: taking actinidia melanandra anther as a material and carrying out in vitro culture; based on an MS culture medium, adding zeatin, auxin, coconut juice or corn stigma juice, sucrose and agar; based on a B5 culture medium, adding naphthalene acetic acid, kinetin, potato juice, the sucrose and the agar as an induction callus medium; successfully obtaining callus of haploid actinidia melanandra after one week. The average induction rate of the method is 72.95 percent and can be up to 88.64 percent which is obviously higher than the haploid occurrence probability of a spontaneous generation pathway; in addition, the method has the advantages of simpleoperation and less time and is a relatively-effective way to obtain actinidia melanandra haploid callus; the method lays a foundation for rapid acquisition of homozygote as well as gene transformation, cell culture and genomic research of the actinidia melanandra.