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Establishing and screening analysis method for panicum miliaceum EMS mutant library

An analysis method and mutant technology, which are applied in the fields of botanical equipment and methods, application, and plant gene improvement, etc., can solve the problems of late start of gene discovery and functional identification of millet, construction of millet mutant library, and report of functional genome identification.

Inactive Publication Date: 2018-11-30
SHANXI AGRI UNIV
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AI Technical Summary

Problems solved by technology

However, the excavation and functional identification of broomcorn millet genes started late in China, and there are no reports on the construction, identification and functional genome of broomcorn broomcorn mutant library

Method used

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  • Establishing and screening analysis method for panicum miliaceum EMS mutant library
  • Establishing and screening analysis method for panicum miliaceum EMS mutant library
  • Establishing and screening analysis method for panicum miliaceum EMS mutant library

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Test materials

[0029] Yi Xuan Big Red Rice

[0030] 2. Experimental design

[0031] 6 concentration gradients were set in the experiment, and 3 replicates were set for each concentration. Each replicate was treated with 300 seeds of Yixuan Dahongsi, and 1 clean water control.

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Abstract

The invention discloses an establishing and screening analysis method for a panicum miliaceum ethylmethylsulfone (EMS) mutant library, and belongs to the field of agricultural breeding. According to the method disclosed by the invention, a technology platform of panicum miliaceum EMS mutagenesis is constructed by screening of an optimal concentration of EMS-processed Yixuan red panicum miliaceum seeds; 103 mutant plant lines are found after obtained 2333 M2-generation plant lines are planted, morphological characters include a leaf color, a leaf type, a plant height, fertility, an ear type, anear color and a grain color, and the mutation frequency is 4.4%; and the mutant library with abundant phenotypes is established, and the mutant library can provide abundant mutant resources and novelgermplasm for functional genomic research and breeding of the panicum miliaceum.

Description

technical field [0001] The invention belongs to the field of agricultural breeding and relates to the construction of a broomcorn mutant library, in particular to a method for establishing a mutant library by EMS induction for broomcorn broomcorn. Background technique [0002] Millet (Panicum miliaceum L.) belongs to the annual Poaceae Panicum, an allotetraploid whose chromosome number is 2n=4x=36. , the characteristic miscellaneous grain crops mainly cultivated by people in semi-arid areas and ideal multiple crops. After the millet is shelled, it is called yellow rice. It is rich in various proteins, starch, fat, etc. needed by the human body. It has high nutritional and health value, and can be processed into a variety of foods to prevent and treat allergies, obesity and other related diseases; in addition, Its stalks and grains are also high-quality livestock forages. Therefore, millet plays an important role in meeting the diversified needs of public food consumption an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/06
CPCA01H1/04A01H1/06
Inventor 张彬王俊杰王喆陈利青韩渊怀李红英禾璐连卜颍雷梦林任元
Owner SHANXI AGRI UNIV
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