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Cultivation method of novel germplasm of colorized brassica oleracea L.var.capitata

A cultivation method and technology for cabbage, which are applied in the field of plant genetics and breeding, can solve the problems that have not been seen before, and achieve the effects of good bulbing, rich phenotype and sweet taste.

Pending Publication Date: 2019-04-23
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, it is possible to quickly create new germplasm of colorful head cabbage by utilizing the heading property of head cabbage and the characteristic of colored leaves of ornamental cabbage. A new variety of colorful head cabbage has been bred; but there are no related reports at home and abroad

Method used

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  • Cultivation method of novel germplasm of colorized brassica oleracea L.var.capitata
  • Cultivation method of novel germplasm of colorized brassica oleracea L.var.capitata
  • Cultivation method of novel germplasm of colorized brassica oleracea L.var.capitata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] In this example, microspore culture is used to obtain new germplasm of colored head cabbage.

[0092] (1) Parent selection: if figure 1 As shown, female parent: kale red wrinkled leaf type inbred line 07-DH-33, which is a DH pure line cultivated by the inventor's research group from the kale variety Imperial Red (F1, purchased from Nongyou Seedling Company) The selfed offspring of , the previous research shows that it has good ability to induce embryoid bodies in microspore culture; its phenotype characteristics are: red (RR, red) wrinkled leaf (CC, curly) scattered leaf (hh) type inbred line (genotype RRCChh). Male parent: high-generation inbred line 15-10 with good taste quality and heading ability of head cabbage, isolated from Zhonggan 21 (commercial F1 variety, Zhongshu Seed Industry Technology (Beijing) Co., Ltd.), characterized by: green ( GG, green) whole leaf (SS, smooth), inbred line (genotype GGSSHH) with good taste quality and head quality (HH, head).

[...

Embodiment 2

[0121] In this example, microspore culture is used to obtain new germplasm of colored head cabbage.

[0122] (1) Parent selection: if Figure 5 As shown, the female parent: the inbred line 05-10 with good taste quality and heading of cabbage, genotype GGSSHH, isolated from "Zhonggan 21" (Zhongshu Seed Industry Technology (Beijing) Co., Ltd.); male parent : Kale red round leaf type inbred line 05-DH-51, genotype RRSShh, is a DH pure line cultivated by the inventor's research group from the kale variety Red Eagle (F1, purchased from Nongyou Seedling Company) The self-bred progeny of , previous studies have shown that it has a good ability to induce embryoid bodies in microspore culture.

[0123] (2) Obtain F1 generation plants: obtain hybrid F1 generation seeds through artificial assisted pollination; then cultivate the hybrid F1 generation seeds to obtain F1 generation hybrid plants; In a growth environment with a night temperature of 20°C / 10°C. Such as Figure 5 As shown, ...

Embodiment 3

[0140] In this example, traditional breeding methods are used to obtain new germplasm of colored head cabbage.

[0141] (1) parent selection: same as embodiment 1.

[0142] (2) Obtain F1 generation plants: obtain hybrid F1 generation seeds through artificial assisted pollination; and then cultivate F1 generation hybrid plants by cultivating the hybrid F1 generation seeds. During the cultivation process, cabbage is in a growth environment with a day / night temperature of 25° C. / 5° C. during the color development period of the cabbage head. Such as figure 1 As shown, the F1 hybrid plants will exhibit red wavy leaves, semi-headed type.

[0143] (3) Obtain F2 generation plants: select 2-3 strong individual plants for self-pollination at the flowering stage of the above-mentioned F1 generation plants, obtain F2 generation seeds, and cultivate F2 generation plants with sufficient material phenotype traits; The parameters are: in a growth environment with a day / night temperature of...

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Abstract

The invention belongs to the technical field of genetic breeding of plants, and specifically relates to a cultivation method of a novel germplasm of colorized brassica oleracea L.var.capitata. The cultivation method is characterized in that parents of the cultivation method comprise brassica oleracea L.var.capitata and ornamental kale; the male parent is the brassica oleracea L.var.capitata, and the female parent is the ornamental kale; or, the female parent is the brassica oleracea L.var.capitata, and the male parent is the ornamental kale; the ornamental kale is b.oleracea L.var.acephala DC.; and more preferably, the genotype of the male parent is GGSSHH, and the genotype of the female parent is RRCChh; or, the genotype of the male parent is RRSShh, and the genotype of the female parentis GGSSHH. According to the invention, when the breeding germplasm is selected, the b.oleracea L.var.acephala DC. with strong ornamental property and cold-resistant property is selected, and the brassica oleracea L.var.capitata with sweet and soft mouth feel, and good balling property and ball shape and suitable for uncooked eating is selected as a parent material, so that the cultivated colorizedbrassica oleracea L.var.capitata has the characteristics that the color variety is rich; the color is bright; the ornamental property is strong; the cold-resistant property is strong; the eating quality is good; the mouth feel is sweet and crisp; the colorized brassica oleracea L.var.capitata is suitable for uncooked eating; and the balling property is relatively good.

Description

technical field [0001] The invention belongs to the technical field of plant genetics and breeding, in particular to a method for cultivating new germplasm of colored head cabbage. Background technique [0002] Heading cabbage (Brassica oleracea L. var. capitata) is a vegetable crop of the genus Brassica in the family Brassicaceae, with leaf bulbs as edible product organs. Its leaves are oval and entire, and the leaf surface is covered with wax powder. The kind on the international market at present has only the head cabbage of green leaf bulb and the head cabbage of purple-red leaf bulb (trade name: purple cabbage). The latter has purple-red leaves, firm balls, thicker leaves, and harder texture. It has a harder taste when eaten raw or fried, and has no obvious sweetness. The weight of a single ball is about 1.5-2Kg. The suitable growth temperature of the above-mentioned head cabbage is 10-25°C. [0003] Kale (B. oleracea L. var. acephala DC.) and cabbage belong to the B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02C12N5/04
CPCA01H1/02C12N5/04
Inventor 郭宁韩硕刘凡王桂香康俊根宗梅
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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