The invention relates to an injection esomeprazole composition. The injection esomeprazole composition comprises an active component and assisting agents, the active component is esomeprazole or esomeprazole sodium or an esomeprazole crystalline hydrate, the injection esomeprazole composition also comprises slow release microspheres containing the active component, the slow release microspheres comprise 10-30wt% of the active component, 60-80wt% of a biodegradable carrier material and 0.2-10wt% of a stabilizer, and the assisting agents comprise an excipient and a pH adjusting agent; and the injection esomeprazole composition is obtained through lyophilizing the active component, the assisting agents, the slow release microspheres and an aqueous solution having a pH value in a range of 11.0-12.0. The injection esomeprazole composition is a dual-controlled-release proton pump inhibitor releasing drugs two times, increases the treatment indexes of the drugs, and raises the qualities of preparation products.