The invention discloses an in-vitro semi-
dynamic simulation digestion method and device for aquatic products, and belongs to the technical field of in-vitro
simulation digestion. The method comprisesthe following steps of:
oral cavity simulation, specifically, adding an
oral cavity simulation digestive juice and a CaCl2 (H2O) 2 solution into an
aquatic product, and incubating to obtain an
oral cavity simulation digested product;
stomach simulation, specifically, adding a
stomach simulation digestive juice into an oral cavity simulation digested product, and adding a
hydrochloric acid solutionin batches until the pH value is 2.0-1.2, supplementing 20mL of the
system with water, adding a CaCl2 (H2O)2 solution for incubation to obtain a
stomach simulated digested product; and intestine simulation, specifically, adding intestine simulation digestive juice into the stomach simulation digested product, adjusting the pH value to 6.9-7.4 by adopting
sodium hydroxide, adding water to supplement the
system to 40 mL, and adding a CaCl2 (H2O) 2 solution for incubation to obtain an intestine simulation digested product. The simulated digestive juices are more scientific and comprehensive in formula, the pH value of the
digestion condition is finer, the simulated digestion stage is not unchanged. Therefore, the method and device can better fit the digestion condition of a
human body.