The invention relates to a preparation method of a toilet deodorization and disinfection cleaning solution. The toilet deodorization and disinfection cleaning solution is prepared by the following components by the weight percent: 18-25 of orthoboric acid, 8-16 of liquid soap, 15-19 of borax anhydrous, 12-14 of sodium dichlorisocyanurate, 6-10 of sodium tripoly-phosphate, 5-10 of sodium sulfate anhydrous, 0.5-1 of carboxymenthyl cellulose, 0.5-1 of ammonium dodecylbenzenesulphonate, 6-9 of ammonium carbonate, 8-13 of hydrogen peroxide, 0.5-1 of tasteless perfume and 0.5-1 of spice; and the orthoboric acid, the liquid soap, the borax anhydrous, the sodium dichlorisocyanurate, the sodium tripoly-phosphate, the sodium sulfate anhydrous, the carboxymenthyl cellulose, the ammonium dodecylbenzenesulphonate, the ammonium carbonate and the hydrogen peroxide are all combined together according to the weight percent and put in a stirring machine to be for 60 minutes, the tasteless perfume and the spice are added and stirred to the stirred material for 45 minutes, then the new material is taken out, molded and tableted through a molding and tabletting machine with loading quantity of 100 g and then the molded and tableted material is packaged with a plastic bag or plastic box to obtain the qualified cleaning solution.