The invention discloses a method for purifying sodium glycochenodeoxycholate, comprising the following steps: S1, dissolving crude sodium glycochenodeoxycholate in an inorganic acid solution, filtering, washing with water, and drying to obtain glycochenodeoxycholate Crude cholic acid; S2, dissolving the crude glycochenodeoxycholic acid obtained in S1 in an eluent to obtain a crude glycochenodeoxycholic acid solution. Composed of potassium hydrogen buffer solution and acetonitrile; S3, put the crude glycochenodeoxycholic acid solution obtained in S2 on the chromatography column, elute, collect the glycochenodeoxycholic acid eluent with a purity >98% detected by HPLC, concentrate, Washing with water and drying; S4, dissolving the dried product of S3 in a solvent, adding sodium hydroxide solution, and concentrating to obtain glycochenodeoxycholic acid sodium salt with a purity >98% as detected by HPLC. The purification method of the present invention is simple in operation, stable and reliable, and can prepare sodium glycochenodeoxycholate with a purity greater than 98% in large quantities.