Kit for detecting 15 bile acids in serum and application of kit

Abstract

The invention discloses a kit for detecting 15 bile acids in serum and an application of the kit, and belongs to the technical field of blood detection. The 15 bile acids comprise a cholic acid, a glycocholic acid, a taurocholic acid, a lithocholic acid, a glycolithocholic acid, a taurolithocholic acid, a deoxycholic acid, a glycodeoxycholic acid, a taurodeoxycholic acid, a chenodeoxycholic acid,a glycochenodeoxycholic acid, a taurochenodeoxycholic acid, an ursodeoxycholic acid, a glycoursodeoxycholic acid and a tauroursodeoxycholic acid. The kit comprises an eluent, a calibration product solution, a mixed internal standard working solution, a protein precipitant and a quality control product. When the kit is used for detecting the bile acids in the serum, a to-be-detected sample does notneed derivatization treatment, pretreatment is simple, a sample dosage is small, sensitivity is high, specificity is strong, more types can be detected, the 15 types of bile acid can be simultaneously detected within 6.5 minutes, and the kit can be used for clinical diagnosis and health assessment of serum bile acids.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a kit for detecting 15 kinds of bile acids in serum by using ultra-high performance liquid chromatography tandem mass spectrometry technology and an application thereof. Background technique [0002] Bile acid is the main component of bile. It is a general term for a class of 24-carbon cholic acid hydroxyl derivatives. It is the final product of cholesterol metabolized by liver tissue. When the liver circulation is destroyed, bile acid cannot be reused. Bile acids can be divided into free bile acids and conjugated bile acids according to their structure. Free bile acids in the human body can be divided into primary and secondary bile acids according to their production sites and structures. Primary bile acids are converted from intrahepatic cholesterol, and through a series of reactions, finally generate cholic acid (CA) and chenodeoxycholic acid (Chenodeoxyc...

AI Technical Summary

Problems solved by technology

CN 106841492 A discloses a method for detecting five kinds of free bile acids in serum by high performance liquid chromatography tandem mass spectrometry, but this method has certain defects, such as only detecting five kinds of free bile acids, which has limited significance for disease diagnosis and clinical , after the pretreatment protein precipitation, the supernatant needs to be reconstituted after being lyophilized by a centrifugal concentration and freezing system. The pretreatment method is complicated, and the detection time on the machine is as long as 12.5 minutes, which is difficult to apply in clinical detection

CN 107356694 A discloses a method for efficiently detecting 15 kinds of bile acids in blood. Although the method can detect 15 kinds of bile acids at the same time, it also has certain defects. Filter and air-dry for more than 3 hours, punch a sample on the obtained dried blood film, and then extract it through liquid-liquid extraction and nitrogen blowing concentration. This method uses a relatively large sample size, and the pretreatment time is too long and the method is complicated. Relatively limited

And it takes up to 10 minutes to test on the machine, which is difficult to apply in clinical testing

CN 110596295 A discloses a method for detecting bile acids. Although the method detects as many as 38 kinds of bile acids, it is mentioned that fatty acids in serum, feces, and liver samples can be detected, but the serum and liver samples come from mice and yellow catkins. Fish, the number of species detected in serum, feces, and liver matrix is ​​different, and the biological reference range of each bile acid in different matrix is ​​not specified, and the clinical reference significance is unknown; and human samples are fecal samples, which are not as common as blood samples , not easy to obtain; the sample pretreatment method is relatively complicated, and its clinical application is relatively limited

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