71 results about "Cytidine monophosphate" patented technology

The invention discloses a method for preparing citicoline. The method comprises the following steps of taking choline chloride, phosphate ions and cytidine monophosphate or a precursor thereof as substrates, taking glucose, fructose, sucrose or maltose as an energy donor, adding a small-molecule chemical effect substance and utilizing the yeast-cell whole cells with permeability to catalyze and prepare the citicoline. By building a metabolic network model and metabolic flow analysis, adopting the small-molecule chemical effect substance to regulate and control metabolic flow so as to improve the efficiency of energy self-coupling and choline phosphorylation and utilizing the yeast cells with permeability to efficiently prepare the citicoline, the method has the advantages of greatly increasing product concentration and raising the utilization ratio of the substrates.

The present invention relates to a novel N-acetylglucosamine-2- epimerase and a method for preparing CMP-N-acetylneuraminic acid, more specifically, relates to a N-acetylglucosamine-2-epimerase derived from Bacteroides fragilis NCTC 9343, and a method for preparing CMP-N- acetylneuraminic acid using said N-acetylglucosamine-2-epimerase. According to the present invention, CMP-N-acetylneuraminic acid can be produced economically in a large amount through a one-step reaction using cytidine monophosphate and N-acetyl-D-glucosamine which are inexpensive substrates.

The invention discloses a method for preparing citicoline sodium. The method comprises the following steps: adding oxalic acid into a chlorination calcium water solution, removing the precipitate and water, reacting with cytidine monophosphate under the action of triphosgene, and subsequently purifying the citicoline sodium by using a recrystallization method.

The invention discloses citicoline and a synthetic method thereof.The synthetic method includes subjecting cheap and easily available cytidine, serving as a raw material, and dichlorophosphoryl morpholine to condensation at a 5' position highly selectively so as to obtain 5'-phosphorylmorpholinylcytidine; subjecting the 5'-phosphorylmorpholinylcytidine and phosphocholine chloride calcium to condensation in solvents so as to obtain the citicoline.Total yield is up to 58% only by the two steps.The synthetic method is cheap in and easily available to raw materials, expensive cytidine monophosphate is unused, operation steps are simplified, and reaction scale can be expanded to synthesize 500 g of citicoline.The synthetic method has a potential application prospect by providing a novel citicoline synthetic route.

The invention discloses a method for synthesizing citicoline and citicoline without using calcium phosphorylcholine chloride. Acylmorpholine is used as raw material, reacts with ethylene glycol to obtain ethylene glycol ester phosphoromorpholine, and then directly condenses with cytidylic acid to obtain ethylene glycol ester phosphoryl cytidylic acid, and finally tributylamine to ethylene glycol ester The ethylene glycol ester of phosphoromorpholine is ring-opened to give citicoline. There are three steps in the reaction, and the total yield is 78%. The method has cheap and easy-to-obtain raw materials, avoids the use of expensive and toxic DCC and calcium phosphorylcholine chloride, and the yield does not decrease significantly when the reaction scale is expanded to a scale of 500 g. The application of the present invention provides a new synthesis route for the synthesis of citicoline, and has potential application prospects.

The invention discloses a broiler chicken feedstuff additive. The feedstuff additive comprises the following components in parts by weight: 12-33 parts of adenylate, 5-24 parts of guanylic acid, 8-27 parts of uridylic acid, 14-39 parts of cytidine monophosphate, 5-22 parts of glutamine and 6-14 parts of arginine. The invention also discloses a preparation method and application of the broiler chicken feedstuff additive. According to the feedstuff additive disclosed by the invention, the appetite of animals can be boosted, the animals can be promoted to take food actively, the nutrient demand of young chickens in a best immune state is satisfied, the disease resistance and the disease prevention capability of the young chickens are enhanced, and the survival rate of the young chickens obviously increases, so that the feedstuff additive has a positive significance to a livestock culture industry.

The invention discloses a cigarette filter stick additive capable of reducing harm, as well as an application method of the cigarette filter stick additive. The preparation method comprises the following steps: taking one or more of 5'-cytidine monophosphate, 5'-sodium cytidylate, 5'-potassium cytidylate and poly 5'- cytidine monophosphate as active ingredients; taking silica gel, activated carbon, aluminum oxide and sodium alginate as carriers; loading the active ingredients on surfaces of the carriers by a surface chemical reaction to obtain loaded type materials; adding the prepared loaded type materials into a cigarette filter stick to prepare a composite filter stick; and rolling up the cigarette. The analysis shows that the cigarette with the loaded type materials can effectively reduce content of HCN (hydrogen cyanide) in the smoke by 8-29%. The cigarette filter stick additive capable of reducing harm is safe, stable, simple in preparation method and convenient to industrially produce at large scale.

The invention discloses a preparation method of citicoline sodium, and belongs to the technical field of biopharmacy. The preparation method comprises the steps that phosphorylcholine chloride calcium salt serving as a raw material and benzene are subjected to cbinary azeotrope to remove crystallization water in phosphorylcholine chloride calcium salt; phosphorylcholine chloride calcium salt reacts with sodium carbonate to generate a calcium carbonate precipitate, and filtering is conducted to obtain viscous materials; the viscous materials react with acetylchloride, a product reacts with cytidine monophosphate, and reduced pressure distillation is conducted to obtain residues; crystallization and recrystallizzation are conducted through sodium hydroxide and ethyl alcohol to obtain the product. According to the preparation method, operation is easy, acetylchloride is easy to remove by adding water, all the solvents can be recycled, better environmental friendliness and higher economic benefits are achieved, the purity of the product is high and reaches 98%, and the product is suitable for pharmaceutical purposes.

The invention discloses a production method of a flos traditional Chinese medicine. The production method is characterized in that nutrients are used during the growth of the flos traditional Chinese medicine, wherein the nutrients include the following components in percentage by weight: 30 to 70% of nucleotide salt and 30 to 70% of medium trace element, and exclude glucose or / and amino acids; the nucleotide salt is the sylvite or sodium salt of four 5'-mixed nucleotides and obtained by hydrolyzing ribonucleic acid alkaline, namely, wherein the four 5'-mixed nucleotides are namely 5'-adenylate dipotassium or sodium, 5'-guanylic dipotassium or sodium, 5'-cytidine monophosphate dipotassium or sodium, 5'-uridylic acid dipotassium or sodium; and the medium trace element includes Mn, B and Fe, namely manganese sulfate, borax and ferrous sulfate. The production method of the flos traditional Chinese medicine has the effect reaching and even exceeding the effect of a patent for invention with publication patent number of CN102079666B and patent number of ZL201010514231.6. The method is economic and simple. The obtained flos traditional Chinese medicine is large and uniform in flower, the uniformity is up to 70 to 90%, the output is 20 to 40% higher than that of flos traditional Chinese medicine produced by the traditional method, the main components account for 10 to 25%, the obtained traditional Chinese medicines can be prepared into quality decoction pieces, or active ingredients can be extracted, and the yield is up to 10 to 22%.

The present invention provides methods for generating CMP-sialic acid in a non-human host which lacks endogenous CMP-Sialic by providing the host with enzymes involved in CMP-sialic acid synthesis from a bacterial, mammalian or hybrid CMP-sialic acid biosynthetic pathway. Novel fungal hosts expressing a CMP-sialic acid biosynthetic pathway for the production of sialylated glycoproteins are also provided.