36 results about "Cytidine diphosphate" patented technology

The present disclosure relates to nutritional compositions comprising a neurologic component, wherein, the neurologic component may promote brain and nervous system development and further provide neurological protection and repair. The neurologic component may include phosphatidylethanolamine, sphingomyelin, cytidine diphosphate-choline, ceramide, uridine, at least one ganglioside, and mixtures thereof. The disclosure further relates to methods of promoting brain and nervous system health by providing said nutritional compositions to target subjects, which includes pediatric subjects.

The invention relates to a fluorescent probe, which belongs to the technical field of fine chemical engineering, and discloses a rhodamine fluorescent probe with a pseudo nucleic acid base as a recognition site and preparation thereof and application to a nucleotide image. The ring opening of rhodamine lactone is induced between amino naphthyridine and nucleic acid base through a base analogue complementary hydrogen bond effect, and the selective response of specific nucleotide is realized by using the weakness of electrostatic interaction between imine positive ions generated by a ring opening product and phosphoric acid link negative ions with proper length. The probe shows good selectivity to cytidine diphosphate (CDP) and adenosine triphosphoric acid (ATP). Hela cell copolymerization focal imaging experiments show that the probe can produce a sensitive imaging effect on the nucleotide in the cell, the cell state is good after and before imaging, and the probe has small cell toxicity. Since the probe has good bioavailability and a high-sensitivity imaging effect, the probe is widely applied to medical fluorescent imaging.

The invention discloses an amphipathic Eu (III) complex as well as a preparation method and application thereof in detection for cytidine triphosphate. The structural formula of the complex is as shown in the specification. The amphipathic Eu (III) complex is formed by matching a ligand with Eu (III), wherein a compound which takes octadecyl as hydrophobic tail part and takes 1,4,7,10-tetraazacyclododecane as a hydrophobic head part serves as the ligand. The preparation method is simple and the reaction condition is mild. The amphipathic Eu (III) complex provided by the invention has the characteristics of long fluorescent lifetime, large stokes shift and unsaturated coordination; a super-molecule sensing interface is assembled and formed in a water phase; the energy donor alpha-naphthyl phosphate sodium is introduced into the system and the amphipathic Eu (III) complex serves as an energy receptor for receiving energy and emitting Eu (III) characteristic light; when the cytidine triphosphate is contained in a to-be-detected sample, the alpha-naphthyl phosphate sodium on the surface of the self-assembly body is replaced by the cytidine triphosphate, so that the fluorescence is quenched and the selective identification for the cytidine triphosphate is realized.

The invention discloses a genetic engineering preparation method of cytidine triphosphate. In the method, a culture or a treatment substance of single genetic engineering strain is used as an enzyme source; during catalysis, ammonium chloride, saratin and the like are used for reaction, so that the cytidine triphosphate is generated in a reaction solution and accumulated and is extracted from the reaction solution. The invention has the benefits that: (1) the reaction is easier to control due to the adoption of the microbial catalytic reaction ; (2) the cost of a substrate is lower and the cytidine triphosphate can be produced at low cost; and (3) the reaction is quick and the conversion rate is higher. The genetic engineering preparation method can be widely applied to preparation of the cytidine triphosphat.

The invention belongs to a preparation method of poly i-c and belongs to the technical field of biomedicine. The preparation method includes: utilizing inosine diphosphate to prepare polyinosinic acid, and using cytidine diphosphate to prepare polycytidylic acid; utilizing the prepared polyinosinic acid to react with the polycytidylic acid to obtain a high-purity poly i-c product. Poly i-c is prepared through self-developed high-purity polyinosinic acid and polycytidylic acid, so that prepared poly i-c is high in purity, and treatment difficulty in the process of preparing is lowered.

The invention discloses a preparation method of powdery cytidine triphosphate or a sodium salt of cytidine triphosphate. The preparation method comprises the following steps: mixing and precipitating an acid solution containing cytidine triphosphate or the sodium salt of cytidine triphosphate with methanol of which the volume is 5-20 times of that of the acid solution so as to obtain powdery cytidine triphosphate or the sodium salt of powdery cytidine triphosphate, or the preparation method comprises the following steps: (1) mixing the acid solution containing cytidine triphosphate or the sodium salt of cytidine triphosphate with a first organic solvent of which the volume is 2-20 times of that of the acid solution so as to obtain a first precipitate; (2) mixing the first precipitate with ethanediol or glycerin of which the volume is 2-20 times of that of the first precipitate so as to obtain a dissolved solution; (3) mixing and precipitating the dissolved solution with a second organic solvent of which the volume is 2-20 times of that of the dissolved solution so as to obtain powdery cytidine triphosphate or the sodium salt of powdery cytidine triphosphate.

The invention discloses a PLIspE gene and a coded protein and application thereof. The gene is cloned from Paeonia lactiflora by constructing a cDNA library for the first time, so a gap in separation and cloning of a 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase gene from the traditional Chinese medicine--Paeonia lactiflora is filled in. The PLIspE gene provided by the invention has a nucleotide sequence shown in SEQ ID NO. 1, or a homologous sequence obtained through addition, substitution, insertion or deletion of one or a plurality of nucleotides of the nucleotide sequence shown in SEQ ID NO. 1, or an allele of the gene or a nucleotide sequence derived from the allele. The protein coded by the gene has an amino acid sequence shown in SEQ ID NO. 2 or a homologous sequence obtained through addition, substitution, insertion or deletion of one or a plurality of amino acids of the amino acid sequence shown in SEQ ID NO. 2. The PLIspE gene can increase the content of metabolites of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol in Paeonia lactiflora through biotechnology and has good application prospects in aspects like quality improvement of the medicinal material Paeonia lactiflora and synthetic biology of active components of Paeonia lactiflora.

The invention discloses a method for synthesizing cytidine diphosphate, and belongs to the field of nucleoside synthesis in organic chemistry. The method comprises the following reaction steps that cytidine monophosphate and dichlorophosphoryl morpholine serve as raw materials, condensation is conducted under the base catalysis effect, then a crude product cytidine diphosphate is obtained through acyl chloride hydrolysis, recrystallization is conducted through an alcohol/water mixed system, and a finished cytidine diphosphate product with the HPLC purity larger than 95% is obtained. The whole reaction only comprises two steps, reaction operation is simple, and the total yield reaches 70% or above.

The invention discloses a PLIspF gene and a coded protein and application thereof. The gene is cloned from Paeonia lactiflora by constructing a cDNA library for the first time, so a gap in separation and cloning of a 2-C-methyl-D-erythritol-4-cyclodiphaophate synthase gene from the traditional Chinese medicine--Paeonia lactiflora is filled in. The PLIspF gene provided by the invention has a nucleotide sequence shown in SEQ ID NO. 1, or a homologous sequence obtained through addition, substitution, insertion or deletion of one or a plurality of nucleotides of the nucleotide sequence shown in SEQ ID NO. 1, or an allele of the gene or a nucleotide sequence derived from the allele. The protein coded by the gene has an amino acid sequence shown in SEQ ID NO. 2 or a homologous sequence obtained through addition, substitution, insertion or deletion of one or a plurality of amino acids of the amino acid sequence shown in SEQ ID NO. 2. The PLIspF gene can increase the content of metabolites of 2-phospho-4-(cytidine 5'-phospho)-2-C-methyl-D-erythritol in Paeonia lactiflora through biotechnology and has good application prospects in aspects like quality improvement of the medicinal material Paeonia lactiflora and synthetic biology of active components of Paeonia lactiflora.