36 results about "Cytidine diphosphate" patented technology

The present disclosure relates to nutritional compositions comprising a neurologic component, wherein, the neurologic component may promote brain and nervous system development and further provide neurological protection and repair. The neurologic component may include phosphatidylethanolamine, sphingomyelin, cytidine diphosphate-choline, ceramide, uridine, at least one ganglioside, and mixtures thereof. The disclosure further relates to methods of promoting brain and nervous system health by providing said nutritional compositions to target subjects, which includes pediatric subjects.

The invention discloses a preparation method of a gene DNA (Deoxyribose Nucleic Acid) sequence capture probe. According to the technical scheme, the method comprises the following steps: 1) designing two partially complementary primers and carrying out annealing treatment so as to form a partially double-chain structured split joint, and carrying out a coupled reaction between the split joint and an ordinary PCR (Polymerase Chain Reaction) amplification product (A1) of a target gene so as to obtain a target gene (A2) two ends of which are provided with joints; 2) designing corresponding primers according to the sequences of the joints and amplifying the target gene (A2) so as to obtain an amplification product (A3) with a T7 promoter; and 3) quantifying the product (A3), and adding NTP (Nucleotide Triphosphate) with biotin labels (wherein biotins are labeled on the NTP is labeled with biotins but ATP (Adenosine Triphosphate), CTP (Cytidine Triphosphate) and DTP (Deoxyadenosine Triphosphate) are not labeled with biotins) into the product (A3) under a certain condition so as to carry out an in-vitro transcription reaction, thereby finally obtaining an RNA (Ribose Nucleic Acid) probe with biotin labels (gene DNA sequence capture probe).

The invention relates to an artemisia apiacea4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol kinase coding sequence in the technical field of gene engineering. The sequence is a sequence generated after one or a plurality of codons in nucleotide are replaced by degenerate codons of amino acid with same coding, as shown in 15-pisition to 1214-position of SEQ ID NO: 3 or shown in 15-position-1214-position of SEQ ID NO: 3. The method for improving the content of plant artemisinin by using the coding sequence includes the steps of: connecting the coding sequence to a plant expression regulatory sequence and constructing a plant expression carrier containing the artemisia apiacea4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol kinase coding sequence; transferring the expression carrier in step 1 to agrobacterium rhizogenes, then transferring agrobacterium rhizogenes to artemisia apiacea; through antibiotic screening, obtaining a transformation cell containing the artemisia apiacea4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol kinase coding sequence and regenerating a transgenosis plant. The coding sequence can improve the content of artemisinin in artemisia apiacea and the content of isoprene compound of other plants.

The invention relates to a manufacturing method of disodium cytidine triphosphate, which comprises the following steps: proceeding enzymatic conversation with CMP material; proceeding ion exchange column chromatography; separating and purifying. The enzymatic conversation reaction principle is that: brewers' yeast gets the needed enzyme liquid of the enzymatic conversation reaction; brewers' yeast produces a plurality of biological enzyme as sugar glycolysis enzyme and phosphorylase with high sugar glycolysis ability; the biological energy produced by glycolysis glucose with sugar glycolysis enzyme is transferred to cytidine monophosphate by ATP; cytidine monophosphate and phosphate radical combines to form cytidine triphosphate with the effect of phosphorylase. The method shapes low cost, simple operation, high production, high product purity by optimization, which comprises the following steps: getting enzyme liquid; proceeding enzymatic conversation by adding the main material cytidine monophosphate, monobasic potassium phosphate of proper quantity and glucose; getting product by deposition, ion exchange column chromatography, segregation, purification, refining and vacuum drying.

The invention discloses a PLIspE gene and a coded protein and application thereof. The gene is cloned from Paeonia lactiflora by constructing a cDNA library for the first time, so a gap in separation and cloning of a 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase gene from the traditional Chinese medicine--Paeonia lactiflora is filled in. The PLIspE gene provided by the invention has a nucleotide sequence shown in SEQ ID NO. 1, or a homologous sequence obtained through addition, substitution, insertion or deletion of one or a plurality of nucleotides of the nucleotide sequence shown in SEQ ID NO. 1, or an allele of the gene or a nucleotide sequence derived from the allele. The protein coded by the gene has an amino acid sequence shown in SEQ ID NO. 2 or a homologous sequence obtained through addition, substitution, insertion or deletion of one or a plurality of amino acids of the amino acid sequence shown in SEQ ID NO. 2. The PLIspE gene can increase the content of metabolites of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol in Paeonia lactiflora through biotechnology and has good application prospects in aspects like quality improvement of the medicinal material Paeonia lactiflora and synthetic biology of active components of Paeonia lactiflora.

The invention belongs to a preparation method of poly i-c and belongs to the technical field of biomedicine. The preparation method includes: utilizing inosine diphosphate to prepare polyinosinic acid, and using cytidine diphosphate to prepare polycytidylic acid; utilizing the prepared polyinosinic acid to react with the polycytidylic acid to obtain a high-purity poly i-c product. Poly i-c is prepared through self-developed high-purity polyinosinic acid and polycytidylic acid, so that prepared poly i-c is high in purity, and treatment difficulty in the process of preparing is lowered.

The invention discloses a genetic engineering preparation method of cytidine triphosphate. In the method, a culture or a treatment substance of single genetic engineering strain is used as an enzyme source; during catalysis, ammonium chloride, saratin and the like are used for reaction, so that the cytidine triphosphate is generated in a reaction solution and accumulated and is extracted from the reaction solution. The invention has the benefits that: (1) the reaction is easier to control due to the adoption of the microbial catalytic reaction ; (2) the cost of a substrate is lower and the cytidine triphosphate can be produced at low cost; and (3) the reaction is quick and the conversion rate is higher. The genetic engineering preparation method can be widely applied to preparation of the cytidine triphosphat.

The invention relates to an artemisia apiacea4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol synthase coding sequence in the technical field of gene engineering. The sequence is a sequence generated after one or a plurality of codons in nucleotide are replaced by degenerate codons of amino acid with same coding, as shown in 18-position to 926-position of SEQ ID NO: 3 or shown in 18-position to 926-position of SEQ ID NO: 3. The method for improving the content of plant artemisinin by using the coding sequence includes the steps of: connecting the coding sequence to a plant expression regulatory sequence and constructing a plant expression carrier containing the coding sequence; transferring the expression carrier in step 1 to agrobacterium rhizogenes, then transferring agrobacterium rhizogenes to artemisia apiacea; through antibiotic screening, obtaining a transformation cell containing the coding sequence and regenerating a transgenosis plant. The invention relates to a polypeptide, and the sequence is shown in SEQ ID NO: 4. The coding sequence can improve the content of artemisinin in artemisia apiacea and the content of isoprene compound of other plants.