A kind of preparation method of polymyocyte
A technology of polyinosin and polyinosinic acid is applied in the field of polyinosinic preparation, which can solve the problems of inability to guarantee the quality of the final product, incomplete production process, and incapability of large-scale production, so as to improve product purity and effective ingredients. content, the effect of reducing the difficulty of production
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Embodiment 1
[0031] A preparation method of polymyocytes, comprising the following steps:
[0032] 1. Preparation of cytidine diphosphate CDP solution
[0033] 1) Phosphorylation, put 300 parts by weight of yeast into the phosphorylation reaction tank and stir, heat in a water bath to 31 ° C, stop heating, put in 5 parts by weight of magnesium chloride, 30 parts by weight of potassium dihydrogen phosphate, and 20 parts by weight of white sugar, and control the temperature at 31 °C. react at ℃ for 20 minutes, then add 30 parts by weight of cytidylic acid, and react for 3 hours. When the content of cytidine triphosphate is greater than 85%, the reaction is completed. Immediately adjust the pH to 2 to terminate the reaction, and at the same time, cool with refrigerated water;
[0034] 2) Chromatographic purification, the phosphorylated product after cooling in step 1) is subjected to plate and frame filtration, chromatography, adsorption, washing, elution and decolorization sodium filtration;...
Embodiment 2
[0057] A preparation method of polymyocytes, comprising the following steps:
[0058] 1. Preparation of cytidine diphosphate CDP solution
[0059] 1) Phosphorylation, put 200 parts by weight of yeast into the phosphorylation reaction tank and stir, heat in a water bath to 30°C, stop heating, put in 2 parts by weight of magnesium chloride, 20 parts by weight of potassium dihydrogen phosphate, and 20 parts by weight of white sugar, and control the temperature at 31 React at ℃ for 10 minutes, then add 30 parts by weight of cytidylic acid, and react for 2 hours. When the content of cytidine triphosphate is greater than 85%, the reaction is completed. Immediately adjust the pH to 2 to terminate the reaction, and at the same time, cool with refrigerated water;
[0060] 2) Chromatographic purification, the phosphorylated product after cooling in step 1) is subjected to plate and frame filtration, chromatography, adsorption, washing, elution and decolorization sodium filtration;
[0...
Embodiment 3
[0083] A preparation method of polymyocytes, comprising the following steps:
[0084] 1. Preparation of cytidine diphosphate CDP solution
[0085] 1) Phosphorylation, put 500 parts by weight of yeast into the phosphorylation reaction tank and stir, heat in a water bath to 31 ° C, stop heating, put in 10 parts by weight of magnesium chloride, 50 parts by weight of potassium dihydrogen phosphate, and 50 parts by weight of white sugar, and control the temperature at 33 React at ℃ for 20 minutes, then add 30 parts by weight of cytidylic acid, and react for 5 hours. When the content of cytidine triphosphate is greater than 85%, the reaction is completed. Immediately adjust the pH to 2 to terminate the reaction, and at the same time, cool with refrigerated water;
[0086] 2) Chromatographic purification, the phosphorylated product after cooling in step 1) is subjected to plate and frame filtration, chromatography, adsorption, washing, elution and decolorization sodium filtration;
...
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