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Method of preparing cytidine disodium triphosphate and application

A technology of disodium cytidine triphosphate and cytidylic acid, which is applied in the field of biochemical medicine

Inactive Publication Date: 2007-10-24
BEIJING SL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the characteristics of current CTP production requirements, the present invention invents a production process that can realize large-scale preparation to meet the clinical drug requirements

Method used

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Experimental program
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Embodiment 1

[0039] The preparation of embodiment 1, 20030501 batch samples

[0040] 1. Yeast fermentation process

[0041] Put 100L of culture solution into the 300L fermenter. After sterilizing at 121 degrees Celsius for 30 minutes, cool to 30 degrees Celsius and adjust the pH value to 5.5. Put the above-mentioned secondary seed solution into a fermenter according to the inoculum amount of 10%-20%, culture at 30 degrees Celsius, with an aeration rate of 5 L / min, and stir at 100 rpm for 48 hours. During the cultivation process, when the dissolved oxygen in the fermented liquid stops decreasing or shows an upward trend, a certain amount of glucose and yeast powder are pumped in.

[0042] At the end of the fermentation, keep warm at 28-30 degrees Celsius, and adjust the pH value to 7.0. After 1 hour, the yeast begins to foam and precipitate, and the fermentation broth is centrifuged with a continuous flow centrifuge to collect the supernatant.

[0043] 2. Enzymatic conversion

[0044] P...

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Abstract

The invention relates to a manufacturing method of disodium cytidine triphosphate, which comprises the following steps: proceeding enzymatic conversation with CMP material; proceeding ion exchange column chromatography; separating and purifying. The enzymatic conversation reaction principle is that: brewers' yeast gets the needed enzyme liquid of the enzymatic conversation reaction; brewers' yeast produces a plurality of biological enzyme as sugar glycolysis enzyme and phosphorylase with high sugar glycolysis ability; the biological energy produced by glycolysis glucose with sugar glycolysis enzyme is transferred to cytidine monophosphate by ATP; cytidine monophosphate and phosphate radical combines to form cytidine triphosphate with the effect of phosphorylase. The method shapes low cost, simple operation, high production, high product purity by optimization, which comprises the following steps: getting enzyme liquid; proceeding enzymatic conversation by adding the main material cytidine monophosphate, monobasic potassium phosphate of proper quantity and glucose; getting product by deposition, ion exchange column chromatography, segregation, purification, refining and vacuum drying.

Description

Technical field: [0001] The invention relates to the field of biochemical medicine. The core of the invention is to provide a preparation method of cytidine triphosphate disodium and its preparation for the treatment of concussion and its sequelae, sequelae of cerebral hemorrhage, autonomic disorders, neurosis and cardiac insufficiency, progressive myocardial atrophy, Drug use in hepatitis. technical background: [0002] Cytidine 5′-triphosphate (CTP), also known as CTP, is a nucleotide substance present in the human body, an indispensable substance for life activities, and plays an important role in the normal metabolism of the body. In recent years, the medicinal CTP used clinically is cytidine 5′-triphosphate disodium (cytidine 5′-triphosphate disodium), its chemical name is cytidine nucleoside-5′-triphosphate disodium salt, and its molecular formula is C 9 h 14 N 3 Na 2 o 14 P 3 , with a molecular weight of 527.14, is a nucleotide drug for the treatment of cerebro...

Claims

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Application Information

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IPC IPC(8): C12P19/30A61K31/7068A61P9/00A61P1/16A61P25/00C12R1/85
Inventor 徐明波吴彦卓王勇波卢安京杨仲凡李亚军齐燕明赵紫岭邵兵王俊玲
Owner BEIJING SL PHARMA
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