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Amphipathic Eu (III) complex and application thereof in detection for cytidine triphosphate

A cytosine nucleoside triphosphate and complex technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of narrow application range, low sensitivity, large measurement error, etc., and achieve large Stokes shift, Effects of long fluorescence lifetime and Stokes shift coordination unsaturation

Inactive Publication Date: 2016-01-27
SHAANXI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The traditional identification and detection methods of cytidine triphosphate include enzymatic method, chromatographic separation, chemiluminescence, electrochemical principle, and absorbance photometry. Although the above methods can be used to detect cytidine triphosphate, most methods exist Disadvantages such as high cost, large measurement error, low sensitivity and narrow application range
The fluorescence method is favored by the majority of scientific researchers due to its advantages of simple operation and high sensitivity. A large number of detection methods for cytidine nucleoside triphosphate anions are established by using the change of fluorescence signal, but they can be used for interfacial sensing of cytosine nucleosides. However, it is more challenging to achieve efficient recognition of cytidine triphosphate under physiological conditions, which requires not only differences in the interaction between the sensing unit and different anions, but also the ability to Amplify this differential conversion into easily detectable physicochemical signals

Method used

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  • Amphipathic Eu (III) complex and application thereof in detection for cytidine triphosphate
  • Amphipathic Eu (III) complex and application thereof in detection for cytidine triphosphate
  • Amphipathic Eu (III) complex and application thereof in detection for cytidine triphosphate

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Experimental program
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Effect test

Embodiment 1

[0027] 1. Synthetic Compound 2

[0028] Under nitrogen protection conditions, 1.1789g (1mmol) of compound 1, 0.993g (3.6mmol) of bromoacetamide, 0.366g of potassium iodide (2.2mmol) and 2.5mL (16.8mmol) of triethylamine were dissolved in 50mL of absolute ethanol, Reflux reaction at 90°C for 110 hours. After the reaction, distill off ethanol under reduced pressure, separate by column chromatography (gradient elution with a mixture of chloroform and methanol with a volume ratio of 5:1 to 1:5), and vacuum-dry at 35°C. , to obtain compound 2, its yield is 50.8%, and the reaction equation is as follows:

[0029]

[0030] The structural characterization data of the obtained compound 2 is: 1 H-NMR (400MHz, D 2 O): 3.99 (2H, CH 2 NH), 3.54 (8H, CH 2 CO), 2.76-3.34 (16H, CH 2 Ring), 1.49 (2H, CH 2 ), 1.26 (30H, CH 2 ), 0.84 (3H, CH 3 ).

[0031] 2. Synthesis of amphiphilic Eu(Ⅲ) complexes

[0032] 0.163g (0.25mmol) of compound 2 was added to a flask containing 30mL of dist...

Embodiment 2

[0036] The application of amphiphilic Eu(Ⅲ) complexes in the detection of cytidine triphosphate, the detection method is as follows:

[0037]1. Completely dissolve 1.82 mg (2 μmol) of the amphiphilic Eu(Ⅲ) complex in 20 mL of 10 mmol / L 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH value of 7.0 to obtain 100 μmol / L amphiphilic Eu(Ⅲ) III) complex solution, the solution was kept at 60° C. for 2 hours, cooled naturally to room temperature, and left standing at room temperature for 8 hours to obtain an amphiphilic Eu(III) complex solution forming uniform vesicles. The morphology of the formed vesicles was characterized by dynamic light scattering and transmission electron microscopy. The results are shown in figure 2 , 3 . It can be seen from the figure that the amphiphilic Eu(Ⅲ) complex forms spherical vesicle aggregates with uniform size in the buffer solution, and the average diameter is 70-90nm.

[0038] 2. Add α-sodium naphthalene phosphate to the a...

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Abstract

The invention discloses an amphipathic Eu (III) complex as well as a preparation method and application thereof in detection for cytidine triphosphate. The structural formula of the complex is as shown in the specification. The amphipathic Eu (III) complex is formed by matching a ligand with Eu (III), wherein a compound which takes octadecyl as hydrophobic tail part and takes 1,4,7,10-tetraazacyclododecane as a hydrophobic head part serves as the ligand. The preparation method is simple and the reaction condition is mild. The amphipathic Eu (III) complex provided by the invention has the characteristics of long fluorescent lifetime, large stokes shift and unsaturated coordination; a super-molecule sensing interface is assembled and formed in a water phase; the energy donor alpha-naphthyl phosphate sodium is introduced into the system and the amphipathic Eu (III) complex serves as an energy receptor for receiving energy and emitting Eu (III) characteristic light; when the cytidine triphosphate is contained in a to-be-detected sample, the alpha-naphthyl phosphate sodium on the surface of the self-assembly body is replaced by the cytidine triphosphate, so that the fluorescence is quenched and the selective identification for the cytidine triphosphate is realized.

Description

technical field [0001] The invention belongs to the technical field of supramolecular sensing materials, and in particular relates to an amphiphilic Eu(III) complex and the application of a vesicle-type fluorescent supramolecular sensor assembled by the complex in detecting cytosine nucleoside triphosphate. Background technique [0002] Cytidine triphosphate participates in the biosynthesis process of phospholipids (lecithin, cephalin, serine phospholipid, sphingomyelin, etc.) in the body. Phospholipids are an important component of nerve tissue and a natural fat emulsifier, which can improve brain and nerve tissue, enhance intelligence and memory, and promote the transportation of cholesterol and fat in the body to prevent accumulation. Therefore, it has the effect on cytosine nucleus The identification and detection of glycoside triphosphate is of great significance. [0003] The traditional identification and detection methods of cytidine triphosphate include enzymatic m...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 刘静王波雷海瑞严军林
Owner SHAANXI NORMAL UNIV
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