Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Artemisia apiacea4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol kinase coding sequence

A technology for encoding cytidine diphosphate and alcohol kinase, which is applied to the coding sequence of Artemisia annua 4--2-C-methyl-D-erythritol kinase and the field of coding sequence, can solve problems that have not been reported yet, and achieve the realization of Large-scale production, the effect of increasing the content of artemisinin

Inactive Publication Date: 2010-03-10
SUZHOU TANGJI BIOTECH CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching the literature of the prior art, it is found that there is no report related to the coding sequence of Artemisia annua 4-(5'-diphosphate cytidine)-2-C-methyl-D-erythritol kinase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] Step 1, cloning of Artemisia annua 4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol kinase coding sequence (CMK)

[0025] (1) RNA extraction (RNA extraction)

[0026] Take the leaf tissue of Artemisia annua, grind it in liquid nitrogen, add it to a 1.5mL Eppendorf (EP) centrifuge tube filled with lysate, shake it fully, and extract total RNA according to the instructions of the TIANGEN kit. The quality of total RNA was identified by formaldehyde denaturing gel electrophoresis, and then the RNA content was determined on a spectrophotometer.

[0027] (2) Full-length cloning of the gene (Cloning of Full-length cDNA)

[0028] According to the conserved sequence of amino acids encoded by related genes in Arabidopsis, using the principle of homologous gene cloning, the RACE (Rapid Amplification of cDNA Ends) method (SMART of Clontech Company) was adopted. TM RACE kit) for full-length cDNA cloning in three stages:

[0029] ①Synthesis of first-strand cDNA

[0030] Take a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an artemisia apiacea4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol kinase coding sequence in the technical field of gene engineering. The sequence is a sequence generatedafter one or a plurality of codons in nucleotide are replaced by degenerate codons of amino acid with same coding, as shown in 15-pisition to 1214-position of SEQ ID NO: 3 or shown in 15-position-1214-position of SEQ ID NO: 3. The method for improving the content of plant artemisinin by using the coding sequence includes the steps of: connecting the coding sequence to a plant expression regulatory sequence and constructing a plant expression carrier containing the artemisia apiacea4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol kinase coding sequence; transferring the expression carrier in step 1 to agrobacterium rhizogenes, then transferring agrobacterium rhizogenes to artemisia apiacea; through antibiotic screening, obtaining a transformation cell containing the artemisia apiacea4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol kinase coding sequence and regenerating a transgenosis plant. The coding sequence can improve the content of artemisinin in artemisia apiacea and the content of isoprene compound of other plants.

Description

technical field [0001] The present invention relates to a coding sequence in the technical field of genetic engineering, in particular to a coding sequence of Artemisia annua 4-(5'-cytidine diphosphate)-2-C-methyl-D-erythritol kinase. Background technique [0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin is a sesquiterpene lactone compound containing a peroxide bridge structure isolated from its aerial part. It is currently recognized as the most effective drug for treating malaria in the world, especially for cerebral malaria and chloroquine-resistant malaria. efficient. Currently, the most effective treatment for malaria recommended by the World Health Organization is artemisinin combination therapy (ACTs). In addition, with the gradual deepening of pharmacological research on artemisinin, scientists have found that artemisinin and its derivatives also have anti-inflammatory, anti-schistosomiasis, anti-tumor and immune regulati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/12C12N15/82C12N15/84
Inventor 唐克轩王玥月翁纯纯杨可
Owner SUZHOU TANGJI BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com