Enzymatic synthesis process for 5'-cytidine monophosphate

Abstract

The invention discloses an enzymatic synthesis process for 5'-cytidine monophosphate. The enzymatic synthesis process for 5'-cytidine monophosphate comprises the following steps: synthesis of cytidine: synthesizing the cytidine by using cytosine and D-ribose under the catalytic action of synthetase I, and filtering a solution obtained from reaction to obtain cytidine filtrate; preparation of 5'-cytidine monophosphate mother liquor: synthetizing 5'-cytidine monophosphate from cytidine filtrate and sodium dihydrogen phosphate under catalytic action of synthetase II, stirring obtained reaction liquid for a period of time under the condition of insulating and filtering to obtain 5'-cytidine monophosphate mother liquid; and refined extraction of the 5'-cytidine monophosphate: regulating pH of the 5'-cytidine monophosphate mother liquid, then filtering to obtain a 5'-cytidine monophosphate crude product, and refining the 5'-cytidine monophosphate crude product to obtain the product 5'-cytidine monophosphate. The enzymatic synthesis process for 5'-cytidine monophosphate has the advantages that the production cost of the 5'-cytidine monophosphate can be reduced; the process period is short, and the preparation efficiency of the 5'-cytidine monophosphate can be improved; the total yield of the 5'-cytidine monophosphate can be 90% or above, and the content is greater than 99%; and the 5'-cytidine monophosphate is harmless to the environment, equipment and operators.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to an enzyme-catalyzed synthesis process of 5'-cytidylic acid. Background technique [0002] 5'-cytidylic acid is an important biochemical substance in biological cells and participates in various physiological and biochemical reactions. In industry, 5'-cytidylic acid is mainly used in the manufacture of citicoline, cytidine triphosphate, cytarabine, polyinosin and other drugs. In recent years, it can also be added to milk powder in combination with other nucleotides to enhance the baby's immunity. [0003] There are two main production methods for 5'-cytidylic acid, 1) degraded from RNA (ribonucleic acid), and then separated; 2) starting from cytidine, chemically attaching a phosphate group to its 5' position to form 5'-cytidine '-cytidylic acid. Although the first method can obtain four products at one time, except for cytidylic acid, the other three become by-products, and ...

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Problems solved by technology

Although the first method can obtain four products at one time, except for cytidylic acid, the other three become by-products, and the source of RNA is more restricted, and the separation operation in the production process is complicated and the yield is low; At present, chemical methods are mainly used to synthesize 5'-cytidylic acid in industry. The main disadvantages of this method are high cost of raw materials, many wastes, corrosion of equipment, and it is not conducive to the health of personnel and industrial production.