The invention provides a better and suitable simplified representative unicellular whole-
genome database creating method and application thereof. On one hand, the method can be effectively applied totrace samples or samples with smaller
cell population to perform simplified
genome database creation by optimizing primers and amplifying steps; on the other hand, by means of
enzyme correction treatment, an
enzyme treating step capable of specifically resecting
uridine monophosphate can be applied to a simplified
genome unicellular sample amplifying method, and amplification error rate can be remarkably reduced. Compared with the prior art, the method creatively applies an optimized and improved unicellular
database creating scheme to simplified
genome database creation of the trace samples or the samples with the smaller
cell population; meanwhile, compared with the prior art, the method has the advantages of higher efficiency, simpleness, practicability, accuracy, small loss, low cost,good method
repeatability, low error rate, great suitability for the simplified genome unicellular whole-
genome amplification of the trace samples or the samples with the smaller
cell population and ability in widening sample application ranges; meanwhile, the error rate is reduced, and detection accuracy is improved.