Chilled fresh meat freshness marker and screening and prediction model fitting method and application thereof

A predictive model and freshness technology, applied in character and pattern recognition, food testing, measuring devices, etc., can solve problems such as quality decline and harm to consumers' health

Active Publication Date: 2020-10-27
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on metabolome profiling and freshness biomarkers in chilled fresh chicken storage
During the preservation process of chilled chicken, due to the action of microorganisms, the quality continues to decline, endangering the health of consumers

Method used

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  • Chilled fresh meat freshness marker and screening and prediction model fitting method and application thereof
  • Chilled fresh meat freshness marker and screening and prediction model fitting method and application thereof
  • Chilled fresh meat freshness marker and screening and prediction model fitting method and application thereof

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Embodiment Construction

[0027] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0028] The method for screening and predicting markers in this embodiment includes the following steps:

[0029] 1. Experimental animals

[0030] The experimental animals were 70-day-old Haiyang yellow chicken hens raised by Jiangsu Jinghai Poultry Industry Group Co., Ltd., a total of 32, and the feeding conditions were the same.

[0031] 2. Materials and methods

[0032] 2.1 Instruments and reagents

[0033] Experimental instruments include: ultra-high performance liquid chromatography (Agilent 1290), Q Exactive Orbitrap high-resolution mass spectrometer (Thermo Fisher), high-speed low-temperature centrifuge (Eppendorf), vibrating vortex instrument, 4 ℃ refrigerator, pure water instrument (TKA ).

[0034] Experimental reagents include: acetonitrile (CMW, LCMS grade), ammonium acetate (CMW, LCMS grade), ammonia water (CMW, LCMS grade), meth...

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Abstract

The invention discloses a metabonomics-based chilled fresh meat freshness marker and a screening and prediction model fitting method and application thereof. The marker is prepared from indole-3-formaldehyde, uridine monophosphate, phenylmercaptouric acid, gluconic acid, tyramine and serine-phenylalanine. The prediction model is: Y=3.964+1.97E<-7>X1- 4.22E<-7>X2-3.37E<-7>X3+8.80E<-8>X4+1.26E<-8>X5-5.57E<-7>X6. According to the method, an Agilent 1290 UHPLC is connected with a Q Exactive Orbitrap high-resolution mass spectrum in series, the method has higher resolution, more substances can be detected more accurately, metabolites in the preservation process of the chilled fresh chicken can be illustrated more comprehensively, and the obtained result is more reliable.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to chilled meat freshness markers and screening and prediction model fitting methods and applications thereof. Background technique [0002] Chilled meat is also called chilled meat, sour meat, and chilled meat. It refers to the rapid cooling of the carcass after slaughter, so that the temperature of the carcass (the center of the hind leg meat is the measurement point) is reduced to 0-4°C within 24 hours, and it is always kept at 0-4°C during the subsequent processing, distribution and sales. Fresh meat within 4°C. [0003] Chilled chicken refers to live chickens that have been quarantined. After slaughtering, they are rapidly cooled to keep the central temperature of the chicken carcass at 0-4°C, and then they are divided, trimmed, packaged, and kept in good condition during subsequent storage, transportation and sales. Fresh chicken in the range of 0-4°C. The safety con...

Claims

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Application Information

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IPC IPC(8): G01N30/86G01N33/12G06K9/62G06F17/18
CPCG01N30/8693G01N33/12G06F17/18G06F18/24323
Inventor 张涛张闪闪陈兰谢恺舟张跟喜王金玉戴国俊巩用双
Owner YANGZHOU UNIV
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