Method for producing uridine diphosphate glucose and special engineering bacteria for method

Abstract

The invention discloses a method for producing uridine diphosphate glucose and special engineering bacteria for the method. The method for producing the uridine diphosphate glucose comprises the following steps of by using uridine monophosphate and sucrose as raw materials, producing the uridine diphosphate glucose under the action of the engineering bacteria, wherein the engineering bacteria arerecombinant bacteria for expressing functional protein and are obtained by introducing a gene for encoding the functional protein into starting bacteria, and the functional protein comprises polyphosphokinase, uridine monophosphate kinase and sucrose synthase. The method is of great significance to industrial production of the uridine diphosphate glucose.

Description

technical field [0001] The invention relates to a method for producing uridine diphosphate glucose and special engineering bacteria thereof. Background technique [0002] Uridine diphosphate glucose, referred to as UDP-glucose or UDPG, is a kind of nucleotide sugar widely distributed in microorganisms, animal and plant cells. In organisms, it is used in the biosynthesis of various glycosides, oligosaccharides and polysaccharides as a glucose donor. In addition, as an important intermediate product in the interconversion of monosaccharides or the formation of furfuric acid, it plays a central role in carbohydrate metabolism. [0003] The synthesis methods of uridine diphosphate glucose are mainly chemical synthesis, fermentation and enzymatic conversion. Chemical synthesis requires the protection and deprotection of the active groups on the sugar groups. The catalyst is expensive, the reaction conditions are harsh, and a large amount of organic solvents are used in the reac...

AI Technical Summary

Problems solved by technology

Chemical synthesis requires the protection and deprotection of the active groups on the sugar group, the catalyst is expensive, the reaction conditions are harsh, and a large amount of organic solvents are used in the reaction process, which makes the total cost of the chemical synthesis method high and the environment is not friendly

Fermentation often takes a long time and yields are low

There are two main methods for enzymatically synthesizing uridine diphosphate glucose. One is to use uridine triphosphate and glucose-1-phosphate as substrates, which are generated under the action of corresponding pyrophosphorylases. For example, Li Xinliang et al. Phosphoric acid and maltodextrin are used as raw materials, Escherichia coli expressing uridine diphosphate glucose pyrophosphorylase and maltodextrin phosphorylase are used as biocatalysts to produce uridine diphosphate glucose through biotransformation, and uridine diphosphate can be produced at most within 38 hours Glucose phosphate is 42.5g, but the reaction is carried out in two steps, and a large amount of squeezed yeast needs to be added, the reaction time is long and the production cost is high

Another production method is to use uridine diphosphate as a substrate, catalyze the synthesis of uridine diphosphate glucose by sucrose synthase, catalyze 84.1mM (39g) uridine diphosphate to generate 40.9g of uridine diphosphate glucose in 10 hours, However, the high cost of the substrate also limits its industrial production and use.

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