30 results about "Uridine diphosphate glucose" patented technology

The invention discloses a genetically engineered bacteria used for producing stevia glycosyltransferase UGT76G1; a UGT76G1 coding gene is inserted between the restriction enzyme cutting sites EcoRI and XhoI of a PYes2 carrier, so as to construct a recombinant plasmid, and then the recombinant plasmid is introduced to expression host Saccharomyces cerevisiae YPH499 to obtain the engineered bacteria; and the coding gene of the UGT76G1 is GenBank, No. GenBank: AY345974.1, and the gene sequence of the coding gene is named as UGT (Udp Glucuronyl Transferase). The invention also discloses a construction method of the genetically engineered bacteria and the application of the bacteria to the production of rebaudioside A. According to the invention, under the condition that expensive UDPG (Uridine Diphosphate Glucose) is not added, cheap carbon source glucose is used as a substrate, the metabolic pathway of UDPG in the yeast is regulated, and then the rebaudioside A is produced from St glycosides through whole cell catalysis.

The invention discloses a method for generating rebaudioside M by catalyzing rebaudioside A via immobilized glycosyltransferases, which comprises the steps of: (1) crosslinking glycosyltransferase UGT1 and glycosyltransferase UGT2 on activated chitosan beads to obtain immobilized glycosyltransferases; (2) adding rebaudioside A into a container, adding uridine diphosphate glucose, and adding the immobilized glycosyltransferases, and catalyzing obtain rebaudioside M. The immobilized glycosyltransferases according to the invention are easy to separate, can be repeatedly used, have high thermal stability and high operation stability, and can, when compared with free enzymes, directly yield a higher amount of rebaudioside M by catalyzing rebaudioside A via the immobilized glycosyltransferases;the free enzymes can only be used once, while the immobilized glycosyltransferases can be reused; after the immobilized glycosyltransferases are reused 10 times, the activity of the immobilized glycosyltransferases is still more than 50% of that of the immobilized glycosyltransferase used for the first time. The method is simple and easy to implement. The process steps are reduced, the cost is reduced, and the production efficiency is improved.

The invention provides a preparation method of tea containing salidroside. The method comprises the following steps: spraying p-hydroxyphenylethyl alcohol to growing camellia sinensis leaves, and picking to obtain the tea containing salidroside. The method provided by the invention is simple in step; a synthetic precursor, namely p-hydroxyphenylethyl alcohol, of the salidroside is directly supplied from the external world; and uridine diphosphate glucose and glucosyltransferase contained in the tea are taken as raw materials, and the salidroside is synthesized in a tea body. The prepared tea containing the salidroside and application of the tea are further protected in the invention.

The invention discloses optically active (P)-bicyclol-9-O-beta-D-glucoside and (M)-bicyclol-9-O-beta-D-glucoside applicable to medicines and a preparation method and application thereof. According tothe invention, bicyclol and uridine diphosphate glucose disodium salt are catalyzed by glycosyltransferase to synthesize optically active (P)-bicyclol-9-O-beta-D-glucoside and (M)-bicyclol-9-O-beta-D-glucoside in one step. The bicyclol-9-O-beta-D-glucoside prepared according to the invention can be used for preventing and treating liver diseases.

The invention discloses a method for producing uridine diphosphate glucose and special engineering bacteria for the method. The method for producing the uridine diphosphate glucose comprises the following steps of by using uridine monophosphate and sucrose as raw materials, producing the uridine diphosphate glucose under the action of the engineering bacteria, wherein the engineering bacteria arerecombinant bacteria for expressing functional protein and are obtained by introducing a gene for encoding the functional protein into starting bacteria, and the functional protein comprises polyphosphokinase, uridine monophosphate kinase and sucrose synthase. The method is of great significance to industrial production of the uridine diphosphate glucose.

The invention provides grifola frondosa UDP (User Datagram Protocol) glucosyltransferase as well as a coding gene and application thereof, and belongs to the field of edible mushroom molecular biotechnology and genetic engineering. According to the invention, UDP (User Datagram Protocol) glucosyltransferase UGT88A1 is obtained by cloning from Grifola frondosa and is named as GUFGT88A1, and the amino acid sequence of the UDP glucosyltransferase UGT88A1 is shown as SEQ ID NO. 2; the UDP glucosyltransferase is glucosyltransferase on which uridine diphosphate glucose (UDP-glucose) depends; the UDP glucosyltransferase can be used for catalyzing polysaccharide synthesis.

A recombinant microorganism for producing 2,3-butanediol consisting of selecting at least three groups from uridine diphosphate glucose phosphate uroglycan transferase gene (galU), acetyl alcohol dehydrogenase gene (acoA), acetyl phosphate transferase gene (pta), adenosine glucosylphosphate transferase gene (glgC), lactose dehydrogenase gene (ldhA), and phosphodiesterase gene (pdeC) which were modified.

The invention relates to a method for preparing rebaudioside D by fermentation catalysis of bacillus subtilis, which is characterized by comprising the following steps: (1) inoculating glycosyl transferase UGT76G1 and AtSUSY genes to a PBR322 plasmid vector, guiding into the bacillus subtilis, inoculating into an LB culture medium, and culturing for 20-24 hours at the temperature of 30-37 DEG C and the speed of 100-250 rpm; (2) inoculating into a seed tank according to the inoculation amount of 1%-10%, and culturing for 20-24 hours at the temperature of 30-37 DEG C at the speed of 200-400 rpm and the ventilation ratio of 0.1-1 V/V.min; (3) inoculating into a fermentation tank according to the volume ratio of 1%-10%, and culturing for 48-72 hours under the conditions that the speed is 200-1000 rpm, the ventilation ratio is 0.1-2 V/V.min, the temperature is 30-37 DEG C and the pH value is 6-8; (4) carrying out ultrafiltration on the fermentation liquor by adopting a ceramic membrane, resuspending bacteria, carrying out high-pressure homogeneous crushing, and carrying out ultrafiltration on the crushed liquor by adopting the ceramic membrane to obtain crude enzyme liquor; and (5) mixing stevioside, uridine diphosphate glucose, a Tris-HCl buffer solution and the crude enzyme solution according to a mass ratio of (5-10): (1-5): (40-60): (5-20), and reacting at 25-40 DEG C for 12-48 hours. The method has the advantages that two crude enzymes are obtained through one-time fermentation, the cost is low, and the conversion rate of rebaudioside D reaches 88-90 g/L.