Cultivation method and application of a plant containing salidroside

A salidroside and cultivation method technology, applied in the field of biotransformation, can solve the problems of many by-products, high raw material loss, high cost, etc., and achieve the effects of reduced usage, convenient operation, and easy operation

Inactive Publication Date: 2017-09-29
HUNAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main function of β-glucosidase in organisms is to catalyze the hydrolysis of glycosidic bonds, and the synthesis of salidroside uses its reverse reaction, which is limited by thermodynamic control and reaction balance, and the yield is low
[0007] Salidroside can also be synthesized by chemical method, but because the chemical synthesis process needs to go through multi-step protection and deprotection measures, the process is complex and costly, there are many by-products and difficult to separate, and it is more harmful to the environment, so no major mass production
[0008] In summary, the current synthetic methods of salidroside have not been widely used in industry due to reasons such as high production cost, low yield, and complicated synthetic steps.
Although there are reports in the prior art about adding specific raw materials to the water body or soil where plants grow, and generating salidroside through biotransformation, this addition method requires real-time monitoring and continuous replenishment of substances in the water body or soil, and the operation It is complex, and a large amount of added raw materials is lost, most of which cannot be absorbed, the conversion efficiency is not high, and it has a certain toxic effect on the plant root system, affecting root growth

Method used

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  • Cultivation method and application of a plant containing salidroside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Motherwort Containing Salidroside

[0039] 1. Cultivation method: Spray 0.5% p-hydroxyphenylethanol aqueous solution to the leaves of the motherwort grown in hydroponics, and harvest the motherwort leaves on the third day.

[0040] A control group is set at the same time, the difference between the control group and the present embodiment is only that p-hydroxyphenethyl alcohol is not contained in the solvent sprayed to the blades.

[0041] 2, the detection of salidroside: the motherwort leaves obtained in this embodiment and the control group were cleaned and chopped, as two groups of samples to be tested; using high performance liquid chromatography, 5g of samples to be tested were respectively taken, and 25ml of 50% methanol was added , weighed, heated and refluxed for 60 minutes, allowed to cool, made up for lost weight, filtered, and used as two groups of test solution. Take another salidroside standard substance, add 50% methanol to make a solution con...

Embodiment 2

[0043] Example 2: Motherwort Containing Salidroside

[0044] 1. Cultivation method: Spray 10% p-hydroxyphenylethanol aqueous solution to the leaves of the motherwort grown in hydroponics, and harvest the motherwort leaves on the 15th day.

[0045] A control group is set at the same time, the difference between the control group and the present embodiment is only that p-hydroxyphenethyl alcohol is not contained in the solvent sprayed to the blades.

[0046] 2. Detection of salidroside: the detection method is the same as in Example 1.

[0047] It can be seen from the test results that the salidroside content (fresh weight) in the motherwort leaves obtained in this example is 0.028%, and no salidroside can be detected in the control group.

Embodiment 3

[0048] Example 3: Motherwort Containing Salidroside

[0049] 1. Cultivation method: Spray 5% p-hydroxyphenylethanol aqueous solution to the leaves of the motherwort grown in hydroponics, and harvest the motherwort leaves on the 7th day.

[0050] A control group is set at the same time, the difference between the control group and the present embodiment is only that p-hydroxyphenethyl alcohol is not contained in the solvent sprayed to the blades.

[0051] 2. Detection of salidroside: the detection method is the same as in Example 1.

[0052] It can be seen from the test results that the salidroside content (fresh weight) in the motherwort leaves obtained in this example is 0.032%, and no salidroside can be detected in the control group.

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Abstract

The invention provides a method for cultivating plants containing salidroside. The method comprises the following steps: spraying p-hydroxyphenylethanol on growing plant leaves, and harvesting to obtain plants containing salidroside. The method provided by the invention has simple steps. The synthetic precursor of salidroside, p-hydroxyphenylethanol, is directly supplied from the outside, and the uridine diphosphate glucose and glucosyltransferase contained in the plant itself are used as raw materials to synthesize the salidroside in the plant. Tianglycoside. The present invention further protects the application of the salidroside-containing plants obtained through cultivation.

Description

technical field [0001] The invention relates to the technical field of biotransformation, in particular to a method for cultivating plants containing salidroside. Background technique [0002] Salidroside (salidroside) is the most important medicinal ingredient of Crassulaceae (Crassulaceae) Rhodiola (Rhodiola L.) plant, which has anti-fatigue, enhanced immunity, cardiovascular protection, anti-hypoxia, anti-aging, Protect nerve cells, protect the liver, anti-oxidation, anti-tumor, anti-radiation and other specific effects. It has extremely important application value in military, aerospace, sports and health care medicine. In recent years, there are more and more types of products such as pharmaceuticals, beverages, food and cosmetics produced with it as the main raw material, and people's demand for salidroside is increasing. [0003] Rhodiola plants are the main source of extracting salidroside, but wild resources are scarce, and the content of salidroside is very low, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G7/06A01N31/08A01P21/00
Inventor 谭朝阳于静何华妙彭菲崔培梧徐德宏鲁耀邦林艳
Owner HUNAN UNIV OF CHINESE MEDICINE
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