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97 results about "Rebaudioside M" patented technology

Non-caloric sweeteners and methods for synthesizing

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Owner:CONAGEN INC

Non-caloric sweeteners and methods for synthesizing

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Owner:CONAGEN INC

Rebaudioside M purification method

The invention relates to a rebaudioside M purification method with the stages of crude extraction and subsequent refining treatment. The crude extraction stage comprises the following steps: a feed solution flows through eight macroporous absorption resin columns connected in series, and is absorbed sequentially; staged ethanol desorption is carried out, and analysis is carried out; an eluent is subjected to reduced-pressure concentration under high temperature; and an obtained solid is subjected to vacuum drying, such that crude rebaudioside M is obtained, wherein a crude rebaudioside M mass content reaches 25-35%. The subsequent refining treatment comprises the following steps: a mixed solvent is heated; the crude rebaudioside M is dissolved into the mixed solvent, such that a solution is formed; the temperature is reduced to normal temperature; standing and intermittent stirring is carried out; the product is allowed to stand for several hours, and solid-liquid separation is carried out; and the obtained solid is dried, such that refined rebaudioside M is obtained. The refined rebaudioside M content can be higher than 95%. The sweetness of the rebaudioside M is more than 300 times that of cane sugar. The sweetness is more long-lasting than that of cane sugar, and causes no bitter after-taste. The mouthfeel is closer to white sugar, and mouthfeel and taste quality are good. Rebaudioside M is an ideal natural sweet additive.
Owner:QINGDAO RUNDE BIOTECH

Glycosyl transferase mutant and method for catalytically synthesizing rebaudioside M by using glycosyl transferase mutant

The invention discloses a glycosyl transferase mutant and a method for catalytically synthesizing rebaudioside M by using the glycosyl transferase mutant. The mutant is obtained by performing mutation on the basis of a glycosyl transferase amino acid sequence shown as SEQ ID NO: 1, performing induced expression on a mutant strain to obtain a mutant enzyme, and catalyzing 20g/L RebE to synthesize 12.8 g/L RebM by using the mutant enzyme as a catalyst and the enzymic method. The kinetic parameters of the mutant S195Q on rebaudioside E and rebaudioside D and the Michaelis constant of the mutant are 56.34 +/-2.02 mu M and 214.48 +/-14.54 mu M respectively, and are 1/3 and 2/5 of those of a wild type. The glycosyl transferase mutant is coupled with sucrose synthase to realize efficient catalytic synthesis of rebaudioside M. According to the present invention, the recombinant strain of the glycosyl transferase UGT76G1 or the mutant thereof and the sucrose synthase is constructed so as to achieve the efficient catalytic synthesis of the rebaudioside M; the method has the optimal yield in the current enzymatic catalytic synthesis experiment of rebaudioside M, and is green, environment-friendly and pollution-free.
Owner:XINGHUA GL STEVIA CO LTD

Synthesis method of rebaudioside M, intermediate product of rebaudioside M and synthesis method of intermediate product of rebaudioside M

The invention belongs to the field of food chemical industry, relates to a synthesis method of rebaudioside M, an intermediate product of the rebaudioside M and a synthesis method of the intermediate product of the rebaudioside M, and particularly relates to a process method which uses ready-made natural products and non-toxic reagents and is applicable to industrial production. The provided synthesis method of the rebaudioside M is bran-new. The synthesis method provided by the invention comprises the following routes: (1) protecting hydroxyl groups of a compound A, so as to obtain a compound B; (2) enabling the compound B and a compound C to react in the presence of a phase transition reagent and an inorganic base, so as to obtain a compound D; (3) removing protecting groups of R1 and R2 from the compound D, thereby obtaining the rebaudioside M. The method has the advantages that the preparation method is simple and is easy in operation, the purity of a product is high, and the conversion ratio is high.
Owner:成都华高瑞甜科技有限公司
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