The invention discloses an osmanthus flower essence containing natural osmanthus flower fragrance and a preparation method thereof. The osmanthus flower essence mainly comprises natural osmanthus flower fragrance extract, leaf alcohol, isoamyl acetate, cis-3-hexenyl acetate, d-limonene, linalool oxide, linalool, phenethyl alcohol, benzyl acetate, ethyl maltol-1, citronellol, ethyl phenylacetate, geraniol, phenethyl acetate, eugenol, alpha-ionone, dihydro-beta-ionone, gamma-decalactone, beta-ionone, delta-dodecalactone, edible alcohol and like components. The above preparation has the following advantages: the osmanthus flower fragrance extract obtained by low-temperature vacuum distillation serves as a key raw material, and the osmanthus flower essence is prepared by means of an analog-matching formulation. The osmanthus flower essence is featured with verisimilar fragrance, rich body fragrance, strong natural feeling and lasting base fragrance, is applied to food and beverage as food additive, and can bring product natural osmanthus flower fragrance and mouth feel; and meanwhile the fragrance quality and fragrance amount of the osmanthus flower essence are higher than like essence products at home.